LITERATURE


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110% Analysis of Fatty Acids in Butter Using GC x GC-MS
Analysis of Fatty Acids in Butter Using GC x GC-MS
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104% Qualitative Analysis of Triglycerides in Cooking Oil Using GC/MS
The following examples illustrate the use of the combined data from EI and CI for the triglyceride analysis of a butter sample by GC/MS.
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104% Analysis of Low Concentration BPA in Consumer Products by GC/MS without Derivatization
The following poster discusses the analysis of low concentrations of Bisphenol A in conumser products by GC/MS without derivatization.
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97% GC/MS Forensic Toxicological Database
This Application News introduces the usefulness of the forensic substance library in this GC/MS Forensic Toxicological Database.
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94% Applications of Comprehensive GCxGCMS Using a Quadrupole Mass Spectrometer With Ultra-high Scan Speed
A new quadrupole GCMS system, capable of scanning 20,000amu/sec and 100Hz has been developed that meets the data acquisition requirements of the comprehensive GCxGCMS technique. In this study, complex naturally-occurring products will be analyzed that demonstrate the utility of this new GCMS.
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94% Application Book, Fast GC/GCMS, Volume 1
Application Book, Fast GC/GCMS, Volume 1, GCMS in drug screening and clinical chemistry, TLC/MS offline coupling, Higher speed through Fast-GCMS, Determination of oil in water according to DIN H53
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87% Application Book, Fast GC/GCMS, Volume 2
Application Book, Fast GC/GCMS, Volume 2. Fast GC and GC/MS using narrow-bore columns: Principles and Applications, Basics of separation in fast GC
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87% Analysis of Detergent in Diesel Fuel by Gas Chromatography - Mass Spectrometry (GCMS)
Despite initial concerns regarding volatilization of the detergent, high-temperature GCMS chromatographic analysis is a valid technique for determining the concentration of detergent in diesel fuel. The goal of a lower quantitation limit of 125 ppm was exceeded by demonstrating linearity down to 50 ppm.
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87% Analysis of Gasoline Utilizing a GC × GC-MS System
When petrochemical products such as gasoline are analyzed using a GC-MS, a number of paraffin hydrocarbons and aromatic hydrocarbons are detected. Since their peaks overlap, qualitative and quantitative analysis of the respective components can be difficult. Analysis of such samples using a GC × GC-MS system, on the other hand, results in comprehensive 2-dimensional separation, simplifying qualitative and quantitative analysis.
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87% Analysis of Opioids Using Isotope Dilution with GCMS-TQ8030 GC/MS/MS
This application note presents instrument configuration, operating parameters, and analytical results for analysis of a common narcotic, hydrocodone, using the isotope dilution technique paired with the specificity of the MRM analysis mode of the Shimadzu GCMS-TQ8030 triple quadrupole GC/MS/MS. Internal standard calibration of codeine and oxycodone was also included in the study.
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86% Evaluation of Sample Pretreatment Procedures for the Determination of Phthalate Esters in Child Care Products and Children’s Toys by Gas Chromatography / Mass Spectrometry (GCMS)
The Consumer Product Safety Improvement Act of 2008 (CPSIA-USA) requires testing of child care products and toys in the US for specific phthalate esters by GCMS. The CPSC test method specifies solvent extraction and subsequent GCMS analysis for specific phthalate esters. In this study, analysis by pyrolysis GCMS was investigated as a technique to simplify sample preparation and improve analytical precision. The analyses were conducted on a Shimadzu GCMS-QP2010 Ultra equipped with a Frontier PY-2020is pyrolyzer, shown in the figure below.
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83% Sugar and sterol analysis in human body fluids on a true dual column GCMS system
Being flexible is one of the most important services in an analytical supporting laboratories. This escpacially the case in hospitals where fast accurate respons is essential.
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83% Analysis of Detergent Residues on Clothing Using DMI-GC/MS
This datasheet investigates the effectiveness of using GC/MS to analyze non-volatile matrices, by using DMI-GC/MS to analyze detergent residues on clothing.
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83% Analysis of Amino Acids Contained in Vegetable Juice
Amino acids contained in vegetable juice were treated with EZ:faastTM (Phenomenex, Inc.), which enables easy pretreatment, and then analyzed with a GC-MS system.
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83% Analysis of Amino Acids Contained in Miso Soup
Amino acids contained in miso soup were treated with EZ:faastTM (Phenomenex, Inc.), which enables easy pretreatment, and then analyzed with a GC-MS system.
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83% Analysis of Amino Acids Contained in Green Tea
Amino acids contained in green tea were treated with EZ:faastTM (Phenomenex, Inc.), which enables easy pretreatment, and then analyzed by GC-MS.
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83% Analysis of Amino Acids Contained in a Sports Drink
Amino acids contained in a sports drink were treated with EZ:faastTM (Phenomenex, Inc.), which enables easy pretreatment, and then analyzed by GC-MS.
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83% Analysis of Amino Acids Contained in Soy Sauce
Amino acids contained in soy sauce were treated with EZ:faastTM (Phenomenex, Inc.), which enables easy pretreatment, and then analyzed by GC-MS.
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83% Analysis of Adipose Fatty Acids in Human Serum
Adipose extracted from human serum was converted to fatty acid methyl ester, and then analyzed with a GC-MS system. This analysis was performed in conjunction with representatives from Shimane University.
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83% Analysis of Amino Acids Contained in a Grain of Rice
Amino acids contained in a grain of rice were treated with EZ:faastTM (Phenomenex, Inc.), which enables easy pretreatment, and then analyzed with a GC-MS system.
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83% Analysis of Amino Acids Contained in Dashi (Japanese Soup Stock)
Amino acids contained in dashi were treated with EZ:faastTM (Phenomenex, Inc.), which enables easy pretreatment, and then analyzed with a GC-MS system.
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83% Analysis of Amino Acids Contained in Alcohol
Amino acids contained in alcohol were treated with EZ:faastTM (Phenomenex, Inc.), which enables easy pretreatment, and then analyzed with a GC-MS system.
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83% Analysis of Inorganic Gases and Hydrocarbons by GC-MS
This shows an example of analyzing inorganic and hydrocarbons gases using a PLOT (porous layer open tubular) column.
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82% Tetrahydrocannabinol (THC) in Saliva
The National Institute on Drugs of Abuse [NIDA] and The Substance Abuse and Mental Health Services Administration [SAMSHA] determine appropriate detection levels for GCMS confirmation
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81% Improvement of ASTM D-5769 by GCMS High Concentration Tuning and Automated Data Processing
Method ASTM D-5769 is a standard GCMS method to determine aromatics in finished motor gasoline or ethanol blends. However, the intrinsic high sensitivity of mass spectrometry usually gives rise to a limited linear range that makes it difficult to meet method requirements. GCMS-QP2010 Ultra, a new single transmission quadruple GCMS, allows for tuning the instrument in a “high concentration” mode that is suitable for ASTM D-5769 type of analysis without making any hardware compromises. In addition, newly improved Shimadzu aromatics report software is now capable of conducting automated data acquisition, processing and reporting. These new features have greatly enhanced method robustness and efficiency, providing a total solution of running ASTM D-5769.
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79% Profiling of Japanese Green Tea Metabolites by GC-MS
Metabolites from nine varieties of high-grade Japanese green tea leaves which had been ranked in a tea competition were extracted and analyzed by GC-MS. Multivariate data analysis was applied to the GC/MS results; the compounds characterizing the rankings could be verified using a score plot and loading plot. Moreover, a model for predicting rankings was created using PLS regression analysis.
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79% Introduction of a New Fast Scanning, Highly Sensitive Single Quadrupole GCMS: A Platform for Modern Chromatography Techniques
A quadrupole GCMS system, capable of scanning 20,000amu/sec and 100Hz, has been developed that meets the data acquisition requirements of the comprehensive GCxGCMSand Fast-GC techniques. In this study, complex natural product extracts will be analyzed that demonstrate the utility of this new GCMS.
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79% Analysis of Brominated Flame Retardants and Phthalate Esters Under the Same Conditions Using a Pyrolysis GCMS System - TBBPA and BPBPE
This Application Data Sheet shows the results from analyzing TBBPA and BPBPE in polymers under the same analytical conditions as those in Application Data Sheet 47 using the EGA/PY-3030D Multi-Shot Pyrolyzer and GCMS-QP2020 Ultra systems
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79% Analysis of Brominated Flame Retardants and Phthalate Esters Under the Same Conditions Using a Pyrolysis GCMS System (3) - HBCDD
This Application Data Sheet shows the results from analyzing HBCDD in polymers under the same analytical conditions as those in Application Data Sheet 47 using the EGA/PY-3030D Multi-Shot Pyrolyzer and GCMS-QP2020 Ultra.
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79% Analysis of Potential Genotoxic Impurities in Activie Pharmaceutical Ingredients (2)
This Application Datasheet introduces evaluation results with respect to quantification in the GC/MS analysis of sulfonate esters, so-called PGI (potential genotoxic impurities), in active pharmaceutical ingredients (API). Please refer to GC-MS Application Datasheet No. 18, “Analysis of Potential Genotoxic Impurities in Active Pharmaceutical Ingredients,” for the analysis conditions and the total ion current chromatogram for the sulfonate esters.
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79% Determination of Volatile Organic Compounds in Water-based Paints and Varnishes Using GC-MS
A general GC-MS method was developed, using the Shimadzu GCMS-QP2010 Ultra, for determination of 35 VOCs commonly found in water-based paints and varnishes. A wide range of calibration was established with good correlative coefficient, as well as good repeatability. The results even demonstrated the capability of using this instrument for the determination of compounds which were not calibrated, in preparation to fulfil the requirements of any possible future regulations.
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79% Analysis of Di(2-ethylhexyl)phthalate by GC-MS Using Hydrogen Carrier Gas
This data sheet presents the application of hydrogen carrier gas to the analysis of di(2-ethylhexyl)phthalate using the GCMS-QP2010 Ultra, which features a Dual Inlet Turbo Molecular Pump.
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79% Analysis of Organophosphorus Pesticides in Baby Foods Using a Triple-Quadrupole GC/MS/MS System
A Shimadzu GCMS-TQ8030 system operated in the MRM mode was shown to be a rapid, sensitive, and selective technique for analysis of organophosphorus pesticides in baby foods. Reliable, precise measurements were obtained at 1 ng/mL for all 24 OP pesticides. The Shimadzu AART function and GC/MS/MS Pesticide Database simplified development of the MRM method.
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79% Simultaneous Analysis of 66 Pesticides by GC-MS Using Hydrogen Carrier Gas
This data sheet presents the application of hydrogen carrier gas to the simultaneous analysis of a standard sample of 66 pesticides using the GCMS-QP2010 Ultra, which features a Dual Inlet Turbo Molecular Pump.
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79% Analysis of a Benzodiazepine-Based Drug Using GC-MS
Benzodiazepine drugs are commonly used in sleeping aids and tranquilizers, and sometimes in crimes or suicide. Therefore, these chemical substances are often analyzed by forensic laboratories for criminal or academic investigations. This datasheet shows the results from using GC-MS to measure 9 types of benzodiazepine drugs.
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79% Analysis of Gasoline Using a GC-MS
Regular gasoline was analyzed utilizing a GC-MS. To minimize gasoline fuel consumption, the average boiling point is adjusted to about 100oC. In addition, as adjustment to the optimal boiling point depends on the air temperature, the composition will differ between summer and winter, and between cold areas like Hokkaido and hot areas like Okinawa.
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79% Analysis of Soft Drinks Utilizing Headspace GC-MS
With the headspace method, samples are inserted into vials, and the headspace gas is introduced into a GC column, enabling simple measurement of fragrant components. This article introduces the results of the measurement of various soft drinks using Headspace GC-MS.
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79% Analysis of Brominated Flame Retardants Using a Pyrolysis GC-MS System
This datasheet introduces a sample measurement of Decabromodiphenyl ether (Deca BDE), a brominated flame retardant, using the EGA/PY-3030D Multi-Shot Pyrolyzer and the GCMS-QP2010 Ultra.
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79% Analysis of Adipose Fatty Acids in Blood Utilizing a GC × GC-MS System
This application datasheet investigates the separation of the C18 fatty acids: oleic acid and gammalinolenic acid. These substances could not be separated using only a 1-dimensional column, but could be separated 2-dimensionally using a GC × GC-MS system.
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79% Analysis of Impurities in Tests for Residual Solvents in Pharmaceuticals Using GC-MS
This Data Sheet presents an example of the analysis of unknown peaks in a test for residual solvents in pharmaceuticals, using analysis columns that complies with USP467 Procedure A for residual solvents, utilizing the Shimadzu HS-20 Trap headspace sampler, which features a trapping function, and the GCMS-QP2010 Ultra.
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79% Analysis of Inorganic Gas by GC-MS
Currently, inorganic gases are typically analyzed using a gas chromatograph – thermal conductivity detector system. This method usually involves using a packed column as the separation column. If a PLOT (porous layer open tubular) column with packing particles fixed to the column interior wall is used instead, however, similar results can be achieved using a gas chromatograph – mass spectrometer (GC-MS) system.
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73% Tank up in the rapeseed fields - Determination of glycerine, methanol, mono-, di- and triacylglycerides
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70% GCMS-TQ8040 Application Solutions brochure
A general interest brochure that explains the product package solutions available for different application areas such as Food, Environment, Life Science, and Forensics.
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70% Analysis of 2-Alkylcyclobutanones by GC/MS: Detection of Irradiated Foods
In this Application News, we introduce an example of fat can be extracted (beef, pork, chicken, salmon, and Camembert cheese , etc . )
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70% Analysis of Compounds Related to Musty Odor Regulated in Drinking Water Using Headspace - GC/MS
This Application News pertains to the measurement of substances (2-methylisoborneol and geosmin) responsible for the musty (mold-related) odor in tap water.
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69% EPA Method 8270D Analysis Using Narrow-bore GC Columns and Fast Data Acquisition with a Quadrupole GCMS System
The goal of this study was to demonstrate the capabilities of a new, sensitive, fast-scanning GCMS system to optimize productivity for EPA Method 8270D, while maintaining the strict QC measures of the method, optimizing sensitivity, and expanding the dynamic range. A new, sensitive, fast-scanning quadrupole GCMS system was used to significantly reduce the run time for a mixture of 81 analytes. Excellent sensitivity and chromatographic resolution were demonstrated with a significantly reduced run/cycle time.
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69% GC-MS High Sensitivity Technology Using Overdrive Lens
The effects of increased signal intensity and improved signal-to-noise ratio (S/N) were investigated by eliminating noise with the installation of an overdrive lens in the GCMS-QP2010 Ultra, and verifying the effectiveness using simulation and experimentation by GC-MS. The results confirmed that S/N improved through application of the optimum voltage on the overdrive lens. In addition, it was verified from the results of analysis of 5 ppb chlorpyrifos-methyl by the full-scan method that high-sensitivity analysis is possible using the GCMS-QP2010 Ultra equipped with this technology.
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69% Analysis of Pesticides in Citrus Oil Using a Shimadzu GCMS-TQ 8030
Detection of pesticides was demonstrated at low ng/mL (ppb) levels in orange oil using a Shimadzu GCMS-TQ8030 GC-MS/MS. Calibration was conducted using the matrix-matched internal standard procedure. Nine calibration standards were prepared and analyzed in diluted organic orange oil over the range of 5-5000 ng/mL (ppb), although some compounds could not be accurately quantitated at the low concentrations due to matrix interference. Precision and accuracy were demonstrated by replicate analyses of matrix spiked aliquots at 5, 10 and 50 ng/mL.
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69% Analysis of Food Preservatives by GC/MS
Food preservatives are used to prevent adverse changes and rotting of foods by suppressing the proliferation of microorganisms; they are used in accordance with the properties of the food to which they will be added. Many of the analytical methods used for analyzing food preservatives employ extraction of the compounds of interest by the steam distillation technique, followed by quantitative analysis using high performance liquid chromatography (HPLC). Gas chromatography can also be used for measurement of many compounds used as food preservatives, including benzoic acid, sorbic acid, methyl p-hydroxybenzoate (PHBA methyl), ethyl p-hydroxybenzoate (PHBA ethyl), propyl p-hydroxybenzoate (PHBA propyl), and butyl p-hydroxybenzoate (PHBA butyl), and therefore, gas chromatograph / mass spectrometer (GC/MS) is an effective analysis technique for verifying the detection results obtained by HPLC. This Application News introduces an example of GC/MS analysis of six types of food preservatives.
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69% Methods 8260C and 8270D on a Single GCMS without Changing Columns
The Twin Line MS Kit allows the user to install two chromatographic columns in the same mass spectrometer, providing greater flexibility for those laboratories wishing to perform volatile and semi-volatile analyses on a single GCMS unit without breaking the MS vacuum. This configuration incorporates two injection ports and two chromatographic columns simultaneously connected to a single MS. With this configuration, the user can easily switch between volatile and semi-volatile applications without venting the MS vacuum system to change the analytical column.
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69% Analysis of Residual Pesticides in Foods Using Twin Line GC-MS
To obtain highly reliable data from analyzing residual pesticides in foods by GC requires confirmation tests using columns with different liquid phases. In contrast, with GC-MS, confirmation is possible by using not only retention times, but also m/z values. Therefore, normally only one type of column is used for tests. However, due to the increased number of pesticides and the diversification in substances subject to inspection, users are demanding higher reliability by using columns with different liquid phases, even for GC-MS analysis. Therefore, a Twin Line MS Kit, which enables the installation of two types of columns with different liquid phases in one MS, without the use of splitters or flow restrictors, was used to analyze residual pesticides in food.
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69% Paint Thinner Analysis Utilizing Headspace GC-MS
Paint thinners, which are utilized to lessen the viscosity of paints, consist primarily of toluene, and contain other substances such as ethyl acetates and alcohols. As these compounds have anesthetic and excitatory properties, “paint sniffing”, or abuse by intentional inhalation, has become a social problem, leading to regulations targeting the inhalation of paint thinner for such purposes [1, 2]. Since GC-MS systems are often utilized in place of GC in scientific crime detection, this experiment investigated the ability of Headspace GC-MS to measure ethyl acetate, toluene, and methanol.
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69% Measurement of Ethanol in Blood Using Headspace GC-MS
Ethanol in blood is analyzed in the context of scientific crime detection with respect to traffic accidents caused by drinking, and violence, injuries, and homicides associated with alcohol[1,2]. The concentration of ethanol in blood is an important factor in understanding the extent of intoxication. Under the Road Traffic Act and the Order for Enforcement of the Road Traffic Act, an individual is considered to be under the influence of alcohol if the blood ethanol level is 0.3 mg/mL or more. While packed column GC-FID and capillary GCFID are recognized measurement methods, a GC-MS is often used instead. This datasheet investigates the ability of Headspace GC-MS to measure ethanol in blood.
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69% Analysis of Pesticides in Baby Foods Using a GCMS-TQ8030 GC/MS/MS, Part II
This application note presents instrument configuration, operating parameters, and analytical results for analysis of 36 pesticides from various chemical classes in a QuEChERS extract of baby food using the Shimadzu GCMS-TQ8030 triple quadrupole GC/MS/MS. Results were evaluated for calibration linearity, analytical precision, and accuracy in a baby food matrix. Selectivity as a function of variable mass spectral resolution settings on the two sets of quadrupoles, Q1 and Q3, is also discussed.
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69% Analysis of Food Additives – Food Antioxidants
This Application News introduces the results of analysis of the antioxidants BHA, BHT and TBHQ using a gas chromatograph mass spectrometer (GC/MS). The antioxidants were added to a butter sample, and then extracted using a simple pretreatment process. In this analysis, the backflush technique was employed to prevent high-boiling point substances in the actual sample from being introduced into the detector.
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69% Application of Metabolomics Techniques using LC/MS and GC/MS Profiling Analysis of Green Tea Leaves
This Application Note presents the profiling of green tea leaves as an example of one application of the metabolome analysis technique using LC/MS and GC/MS. A search of the substances believed to play roles in product quality was conducted, and a quality prediction model was constructed based on the results.
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69% Determination of Drugs of Abuse in Oral Fluids
The methods in this booklet are a compilation of work collected from working forensics laboratories. Each of these labs is a Shimadzu customer and all of the data was generated using the GCMS-QP2010 series of instruments. Analytes include: Opiates, Cocaine, BZE, Phencyclidine, THC, Methadone
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69% Analysis of Aroma Components in Peach Juice by GCMS Using the OPTIC-4 Multimode Inlet and Mono Trap
The OPTIC-4 is a multimode injection system with a thermal desorption function that enables performing thermal desorption by placing an adsorbent in the injection port liner cup. In combination with the new MonoTrap adsorbent, the system enables analyzing aroma components with high sensitivity.
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69% Analysis of Metabolites in Rat Urine Using Scan/MRM via GC-MS/MS (1)
This Application Datasheet introduces results from the measurement of metabolites extracted from rat urine, utilizing Scan/MRM mode with 5 component MRM measurements added to a Scan measurement method from the GC/MS Metabolites Spectral Database.
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69% Analysis of Phthalate Esters in Polyvinyl Chloride Toys by GC/MS
This Application News introduces an example of GC/MS analysis of 6 phthalate esters in toys. For an example of GC-FID analysis of the same sample, refer to Shimadzu Application News No. G275 "Analysis of 6 Phthalate Esters in Polyvinyl Chloride Toys by GC-FID."
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69% Analysis of Psychotropic Drugs in Whole Blood Utilizing Simultaneous Scan/MRM Measurements (1)
When analyzing medicinal toxicants using GC-MS, the presence of fatty acids and cholesterol, which exist in large quantities in whole blood, can interfere with detection. In fact, profiles for triazolam and etizolam, benzodiazepine psychotropic drugs, overlap with the cholesterol chromatogram, making data analysis difficult with a single GC-MS system.
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69% Analysis of Automobile Cabin Air by Thermal Desorption GCMS Using the OPTIC-4 Multimode Inlet
This datasheet shows the results where volatile organic compounds in automobile cabin air were adsorbed using Tenax TA (2,6-diphenyl-p-phenylene oxide), a sorbent material commonly used for atmospheric analysis, and analyzed by TD-GC-MS.
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69% Analysis of Metabolic Components Contained in Dog Cerebrospinal Fluid
Dog cerebrospinal fluid was analyzed with a GC-MS system after pretreatment and trimethylsilylation (TMS). This analysis was performed in cooperation with Dr. Shigeo Takenaka from the Graduate School of Life and Environmental Sciences, Osaka Prefecture University.
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69% Analysis of Potential Genotoxic Impurities in Active Pharmaceutical Ingredients
Methanesulfonic acid (mesylate), benzenesulfonic acid (besilate), and p-toluenesulfonic acid (tosylate), chemicals used in the process of synthesizing active pharmaceutical ingredients, are likely to generate sulfonic acid ester (Fig. 1) as a reaction byproduct. These compounds are known as potential genotoxic impurities (PGI) and are a significant cause for concern among pharmaceutical manufacturers. This article introduces the analysis of sulfonic acid esters utilizing the GCMS-QP2010 Ultra.
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69% Analysis of Light Oil Using a GC x GC-MS System
GC x GC, the latest in chromatographic technology, achieves high-level separation through direct connection of 2 different types of columns. The following shows an example of light oil analysis using a GC x GC system.
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69% Analysis of Metabolic Components Contained in Puffer Fish Blood Serum
Puffer fish blood serum was analyzed with a GC-MS after pretreatment and trimethylsilylation (TMS). This analysis was performed in cooperation with Dr. Shigeo Takenaka from the Graduate School of Life and Environmental Science, Osaka Prefecture University.
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69% Application of Metabolomics Techniques using LCMS and GCMS Profiling Analysis of Green Tea Leaves
This Application Note presents the profiling of green tea leaves as an example of one application of the metabolome analysis technique using LC/MS and GC/MS. A search of the substances believed to play roles in product quality was conducted, and a quality prediction model was constructed based on the results.
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69% Analysis of Fatty Acids Using PCI-GCMS/ MS
This application data sheet introduces the results of an investigation of sensitivity based on the EI-SIM, PCI-SIM, EI-MRM, and PCI-MRM methods. In addition, in Application Data Sheet No. 86, we introduce the results of an investigation of separation from impurities in the analysis of fatty acids in foods.
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69% GC/MS Quantitative Analysis of Allergens in Cosmetics Using OPTIC-4
Because an estimated 1 to 2 percent of the population has allergies, in 2003 the EU began regulating the use of perfumes and flavour materials that can cause allergic reactions. These substances are restricted to 0.01 % for cosmetic products that are rinsed away (such as shampoo) and 0.001 % for products that are left applied (such as hand creams). Due to these regulations, manufacturers now must quantitatively analyze the concentration of such allergens.
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64% Polymer Analysis Using TGA-GC/MS with CI
In mass analysis, a sample is ionized, the mass numbers are measured and qualitative analysis is performed on the basis of the obtained mass spectra.
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64% GCxG Handbook: Fundamental Principles of Comprehensive 2D GC
GCxG Handbook: Fundamental Principles of Comprehensive 2D GC
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63% Analysis Using UV/Py -GC/MS System
Methods used for weather-induced degradation testing of polymeric materials include outdoor exposure methods and weather meters, however, measurement using such methods can take weeks or months to complete. Using a system consisting of a combination of a micro-UV irradiator (UV-1047Xe) and a GC/MS makes it possible to observe degradation products generated due to photo/thermal-oxidative degradation processes in a short period of time. Chemical changes in polymeric materials following these degradation processes can also be determined.
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63% Quantitative Analysis of Stable Isotopes of Glucose in Blood Plasma Using Quadrupole GC-MS
In order to investigate the dynamics of glucose in living organisms, a stable isotope of glucose is administered, and its concentration in blood and tissues is measured with quadrupole GC-MS. In this study, we conducted a comparative investigation of the influence of the derivatization method on the results obtained in the quantitative analysis of D-glucose-13C6 using quadrupole GC-MS. As a result, it was discovered that aldonitrile-pentaacetylation gives a single derivatization product, and in mass separation performed with MS, it yielded good quantitative results with little influence by the natural isotopes of glucose that are present at high concentrations in living organisms.
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63% Reduction of Emissions and Operating Costs of GCMS Usage with a New Ecology Mode
In this work, daily electrical power consumption was measured and compared to that of typical GCMS operation. Measured power correlated to predicted energy savings and emission reductions very closely. A new feature in GCMS software called “Eco-mode” automatically performs the power- and gas-saving functions at the end of an analytical batch. Eco-mode is used to reduce gas flow and turn off unnecessary heated zones, projecting an estimated 35% in energy savings as well as reduction of carbon emissions by up to 1.1 tons per year.
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63% Analysis of Potential Genotoxic Impurities in Active Pharmaceutical Ingredients (4) - Analysis of Haloalcohols and Glycidol, Part 2
This Application Data Sheet reports on results with respect to a method for quantitating haloalcohols (2-chloroethanol, 2-bromoethanol, and 2-iodoethanol) and glycidol in an active pharmaceutical ingredient (API) utilizing the GCMS system. For the analysis conditions as well as the total ion current chromatogram and mass spectra for the haloalcohols and glycidol, refer to GCMS Application Data Sheet No. 41, "Analysis of Potential Genotoxic Impurities in Active Pharmaceutical Ingredients (3), Analysis of Haloalcohols and Glycidol Part 1."
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63% Analysis of Aroma Compounds in Cheese
Volatile compounds, including aroma compounds, in Parmesan and Blue cheese were analyzed using adsorption and thermal desorption GC/MS (TD-GC/MS). MonoTrap was used as adsorbant. It is a state-of-theart silica monolithic and hybrid adsorbent having a large surface area and properties based on silica, activated carbon (graphite carbon for Mono Trap TD) and the octadecyl functional group. GC-MS equipped with the OPTIC-4 multi-purpose injector was used for thermal desorption. The OPTIC-4 allows direct introduction of desorbed gas into a capillary column without re-adsorption.
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63% Utility of a System Consisting of EGA/PY-3030D and GCMS-QP2010 Ultra: Analysis of Eyeliner and Pyrolysis of a Mural Paint at 1000°C
The analysis of eyeliner and museum quality paint are used to demonstrate the capabilities of a system consisting of an EGA/PY-3030D pyrolyzer and a GCMS-QP2010 Ultra. The eyeliner was first analyzed using evolved gas analysis (EGA-MS). The EGA thermogram shows that the eyeliner contains compounds with a wide range of properties – from volatile organic solvents to nonvolatile polymers. The compositional analysis of each temperature zone on the EGA thermogram was determined using a high-resolution capillary column interfaced to the GC-MS. The high temperature pyrolysis of an ancient mural paint illustrates of the value of pyrolysis-GC-MS to characterize a material of interest.
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63% Automatic Identification and Semi-quantitative Analysis of Psychotropic Drugs in Serum Using “GC/MS Forensic Toxicological Database”
A sample consisting of serum extract from a patient administered with psychotropic drugs was analyzed by GC/MS; identification and semiquantitation of the substances were conducted using the “GC/MS Forensic Toxicological Database”. All three administered substances were identified, and their semi-quantitative values were calculated. The results indicated that it is possible to qualitatively and semi-quantitatively determine drugs using this database.
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63% Aroclors in Human Blood Serum by Triple Quadrupole GC/MS/MS
The Shimadzu GCMS-TQ8030 triple quadrupole GC/MS/MS simplifies detection and quantitation of PCBs at sub-ppb concentrations in complex human blood serum with virtually no interference from the background matrix. Ability to independently optimize each instrument parameter, such as MRM transition, collision energy, and quadrupole resolution, produces the level of sensitivity, selectivity, and accuracy required for TEQ measurements and related programs. The method can be used to screen complex biological samples for individual PCB congeners or Aroclor mixtures, and to estimate total PCB concentrations.
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63% Evaluation of Hydrogen as a Carrier Gas for Gas Chromatography / Mass Spectrometry
The Shimadzu GCMS-QP2010 SE, with its inert, low-nickel source, was used for analysis of a wide range of compound classes using hydrogen as the carrier gas without requiring any changes to the instrument hardware. Chromatography with hydrogen carrier gas was excellent, and retention times were easily reproduced using the constant linear velocity feature of the GCMSsolutions software. MS tuning was essentially equivalent, and passed all acceptance criteria with both hydrogen and helium.
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63% Analysis of Light Oil Utilizing a GC x GC-MS System
GC x GC systems use the latest chromatographic technology to achieve high-level separation through direct connection of 2 different types of columns. This method is optimal for the separation of target substances from complicated matrices that are problematic for ordinary GC or GC-MS systems, and for analysis by type from 2- dimensional chromatogram patterns. It can be used for analysis in a variety of fields, including natural products, foodstuffs, fragrances, environmental science, and petro chemistry.
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63% Analysis of Metabolites Extracted from Human Embryonic Stem Cells Using GC-MS
Metabolome analysis, a comprehensive analysis of the various metabolites generated as biological functions are maintained, is widely used in disease biomarker searches and other investigations. To conduct these investigations, it is necessary to identify the metabolites contained in biological samples. This application data sheet introduces the results of identifying metabolites extracted from human embryonic stem (ES) cells utilizing the GC/MS Metabolite Database Ver. 2, which contains metabolites detected in biological samples, such as serum, urine, and cells.
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63% Analysis of Metabolites in Serum Using GC-MS/MS
Single quadrupole GC-MS provides excellent chromatographic resolution and enables stable measurements, and is therefore widely utilized for metabolome analyses involving the comprehensive analysis of in vivo metabolites. However, biological samples contain many metabolites and various matrices, so separation with single quadrupole GC-MS can be difficult. With triple quadrupole GC-MS/MS MRM, MS separation is performed twice, with Q1 and Q3. This helps remove the impact of overlapping peaks due to interfering components in comparison with scan mode, in which MS separation is performed with a single quadrupole, and thus enables the acquisition of accurate quantitative results with high-sensitivity detection.
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63% Analysis of metabolites in human serum using GC-MS
Metabolome analysis, a comprehensive analysis of the various metabolites generated as biological functions are maintained, is widely used in disease biomarker searches and other investigations. To conduct these investigations, it is necessary to identify the metabolites contained in biological samples. This application data sheet introduces the results of identifying metabolites in human serum utilizing the GC/MS Metabolite Database Ver. 2, which contains metabolites detected in biological samples, such as serum, urine, and cells.
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63% Easy Screening for Residual Pesticides in Foods using GC-MS
In recent years, with increases in the number of pesticides and the diversification of substances under investigation, there have been calls for quick and high-accuracy screening for residual pesticides in foods using GC-MS. The Quick-DB database contains a variety of information on 478 pesticide components (mass spectra, retention indices, and calibration curves). It can be used to quickly calculate semi-quantitative values without using standard samples. A pesticide surrogate is used as the internal standard for the calibration curves. Favorable quantitative accuracy is achieved through quantitative analysis in accordance with the physical properties of each pesticide. In this Data Sheet, residual pesticides in soya beans are analyzed using Quick-DB.
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63% GC-MS Application for Artificial Photosynthesis Research
A variety of analytical methods are utilized to measure reaction products in research into artificial photosynthesis, in which high-energy substances are manufactured using photo catalysts and solar energy. These methods include GC, HPLC, and absorption photometry. Experiments utilizing isotope-labeled samples are an effective way of confirming and clarifying reaction mechanisms. Mass spectrometers demonstrate their worth in such measurements. This Data Sheet presents an example of the use of GC-MS to measure carbon monoxide, methanol, formaldehyde, formic acid, and other substances generated in carbon dioxide photo catalytic reduction under a methanol environment.
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61% GC-MS Analysis of Catalytic Reaction Products of Glucose in Biomass Research
This report presents results obtained by adding a solid acid catalyst to an aqueous glucose solution, heating the solution, and then directly analyzing the resulting reaction solution using GC-MS without pretreatment. At present, in the utilization of woody biomass, the cellulose that comprises approximately 60 % to 70 % of the material can be converted to glucose. The analysis of the catalytic reaction presented in this report revealed that primarily hydroxymehylfurfural is further generated from glucose used as a raw material. Hydroxymehylfurfural is a compound with considerable added value as a raw material for pharmaceuticals and chemicals. The analysis also revealed the existence of lactic acid and other low-grade organic acid compounds. In addition, utilizing the GCMS-QP2010 Ultra, which features a differential vacuum system, enabled acquisition of favorable results even when directly injecting the sample aqueous solution into the GC-MS.
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61% GC-MS Analysis of Catalytic Degradation Products of Lignin Model Compounds in Biomass Research
This report presents an example of degrading a lignin model compound with a solid acid catalyst in an aqueous solution and directly analyzing the resulting solution using GC-MS without pretreatment. Lignin accounts for 20 % to 30 % of woody biomass. However, it is only weakly reactive because of its strong macromolecular structure, and therefore difficult to degrade. Since anisole, phenol, guaiacol, and other lignin degradation products are useful materials, though, research is progressing into degradation methods that provide a high conversion yield. The analysis of the catalytic degradation reaction presented in this report revealed that guaiacol is mainly produced from lignin model compounds. In addition, utilizing the GCMS-QP2010 Ultra, which features a differential vacuum system, enabled the acquisition of favorable results even when directly injecting the sample aqueous solution into the GC-MS.
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59% Agreement between GC and GCMS Chromatograms (Chromatograms obtained at same linear velocity using AFC)
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59% Rapid Screening for Drugs of Abuse Using Higher Column Flow Rates in Gas Chromatography Mass Spectrometry
The need to run more samples in the same amount of time with the same data quality has increased; this poster addresses how certain modifications can be made to increase throughput while retaining high-quality data
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59% Analysis of Potential Genotoxic Impurities in Active Pharmaceutical Ingredients (5): Analysis of Alkyl Halides
Alkyl halides are used as an alkylating agent for raw ingredients in the synthesis of pharmaceuticals or are generated as a byproduct of drug synthesis. They have been identified as potential carcinogens or genotoxins. This Application Data Sheet shows an example of analyzing 18 alkyl halides using headspace-GC-MS.
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59% Analysis of Chelate Compounds Through Direct Sample Introduction
Chelate compounds are generally refractory compounds composed of ligands and metal ions. Therefore, they are difficult to measure with gas chromatographs. To obtain an electron ionization mass spectrum of refractory compounds requires a pure sample that should be introduced by direct introduction (DI). In this Application Datasheet, a chelate with ring-shaped phthalocynanine ligands, which are cross linked to four phthalic imides by nitrogen atoms, is measured using the DI method.
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57% GC/MS Forensic Toxicological Database
In recent years, abuse of stimulants and other illegal drugs, particularly hallucinogens, shows no signs of abating; crime and poisoning events due to medicines, agricultural chemicals, and psychotropic drugs are on the increase, presenting a growing and serious social problem. In crime laboratories and forensic research laboratories, these chemicals are analyzed using a gas chromatograph mass spectrometer (GC-MS). The detection of these harmful drugs by GC-MS requires investigation to determine the optimum analytical conditions and data processing conditions specific to each of the drugs. To eliminate the need to conduct these cumbersome operations, Shimadzu has developed its own proprietary "GC/MS Forensic Toxicological Database," which contains critical information for more than 1000 drugs*, including psychotropic drugs, narcotics, stimulants, and pesticides. With emphasis on harmful drugs of abuse, difficult-to-obtain standard samples of these drugs and their metabolites are registered in the library, making the library ideal for forensic toxicological analysis. In addition, the database contains a library of mass spectra, method files containing the names, quantitation and reference ions, standard mass spectra, and retention indices specific to each of these drugs of abuse. Here we introduce quick and easy techniques for detecting and conducting semi-quantitation of harmful drugs using method files.
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55% An Improved Method of Updating Retention Times for GCMS
A new technique has been developed to update retention times automatically in the data processing software. The technique is based on the use of retention indices, referenced to a hydrocarbon standard (series of n-alkanes). This is a multipoint correction method, so target analytes will always be bracketed by the retention index marker compounds to ensure accuracy of updated retention times for target compounds. Examples illustrate the accuracy of this technique using a subset of US EPA Method 8270D analytes.
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55% Analysis of Phthalate Esters in Children's Toys Using GC-MS
As of February 2009, the US Consumer Product Safety Improvement Act, Section 108, mandated that all manufacturers of children’s toys analyze their products for Phthalate Esters. The method to be used is in accordance with the US Consumer Product Safety Commission (CPSC). In this study, six phthalate esters in children's toys were measured using the GCMS-QP2010 Ultra. The simultaneous Scan/SIM data acquisition technique was utilized, with the SIM data used for quantitation. The data showed excellent calibration curve linearity and repeatability. In two of the toy samples, unregulated plasticizers were identified by scan data, illustrating the efficiency of simultaneous Scan/SIM measurement.
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55% Analysis of Potential Genotoxic Impurities in Active Pharmaceutical Ingredients (3) - Analysis of Haloalcohols and Glycidol, Part 1
Haloalcohols are used as synthetic materials in pharmaceuticals, and are considered potential genotoxic impurities (PGI). In addition, glycidol has been identified as a cancer-causing agent, and has been assigned to Group 2A (probably carcinogenic to humans) in terms of carcinogenic risk by the International Agency for Research on Cancer (IARC). This Application Data Sheet introduces analysis of haloalcohols and glycidol in an active pharmaceutical ingredient (API) using the GC-MS.
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55% Analysis of Brominated Flame Retardants and Phthalate Esters In Polymers Under the Same Conditions Using a Pyrolysis GC-MS System (1): PBBs and PBDEs
In recent years, an analysis method is required to determine not only polybrominated biphenyls (PBBs) and polybrominated diphenyl ethers (PBDEs), which are regulated under the RoHS Directive, but also phthalate esters and other brominated flame retardants not governed by the directive (such as tetrabromobisphenol A, hexabromocyclododecane, and bis(pentabromophenyl)ethane). The method was developed to analyze those compounds under the same analytical conditions using EGA/PY-3030D Multi-Shot Pyrolyzer and GCMSQP2020 Ultra systems. This Application Data Sheet shows the results from analyzing the brominated flame retardants PBB and PBDE using the developed method.
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55% High-Sensitivity Analysis of 2,4,6-Trichloroanisole in Wine Using Headspace-Trap GC/MS
2,4,6-trichloroanisole (TCA) emitted from wine corks can taint wine and cause an objectionable odor. Due to the low threshold value for sensing the odor, highly sensitive measurements are required for monitoring. Conventionally, TCA was measured using methods such as purge and trap, which is very effective in concentrating samples, or thermal desorption. The HS-20 headspace sampler includes a trap function that is able to concentrate headspace gases. This Data Sheet describes an example of high-sensitivity measurement of TCA in wine using an HS-trap GC/MS system.
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55% Analysis of Polycyclic Aromatic Hydrocarbons Using GC-MS
Polycyclic aromatic hydrocarbons (PAHs) are byproducts of burning fuels, such as mineral oils, and are known to be carcinogenic, mutagenic, and teratogenic. These compounds are regulated in many countries as harmful pollutants in environmental water and the atmosphere. In Japan, the "Measuring method manual for toxic air pollutants" and "Measuring method manual for polycyclic aromatic hydrocarbons (PAHs) in exhaust gas emissions" were revised in March 2011. In addition, environmental standards, including fine particle regulations (PM2.5) and fine particles, can include PAHs. Consequently, there is an increasing need to measure PAHs. This application datasheet introduces an example of measuring 36 PAHs.
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55% Flavor/Fragrance Analysis by Fast-GC/MS
Fast-GC/MS is an effective way to improve laboratory productivity by shortening analysis cycle times. Fast-GC/MS methods require a system with a high-performance AFC controller that is compatible with narrow bore capillary columns and high-speed data acquisition technology. Shimadzu GCMS systems offer the high-performance levels necessary to satisfy these requirements. This Application Datasheet shows results from analyzing lavender oil using conventional methods and Fast-GC/MS methods.
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55% Analysis of Specific Aromatic Amines Formed From Azo Dyes and Pigments
Reductive cleavage of azo radicals from azo dyes and azo pigments comprising azo radicals (-N=N-) can form aromatic amines that are suspected carcinogens. Of these aromatic amines, 22 are designated as specific aromatic amines in Europe (EN 14362:2003) and 24 in China (GB/T 17592-2006), and the use of azo dyes and pigments that form these specific aromatic amines are regulated. The total ion current chromatogram (TIC) obtained from using GC-MS to measure a standard sample of the specific aromatic amines is shown here.
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55% Analysis of Gas Generated from Lithium Ion Rechargeable Battery Cells
Electrolytic solutions in lithium ion rechargeable batteries consist of organic solvents (mainly composed of the carbonate series), electrolytes, and additives. GC-MS systems are effective for the analysis of the denatured components of electrolytic solutions resulting from the charging and discharging of batteries. This article introduces a sample analysis of gas produced from a lithium ion rechargeable battery cell stored for 5 days at 80oC.
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55% Analysis of Least Volatile Compounds through Direct Sample Introduction
Direct sample introduction (DI) is a method of directly introducing a sample to an ion source without passing it through a gas chromatograph (GC). DI is an effective method for mass spectral measurements of synthesized materials. The Shimadzu GC-MS can use direct sample introduction without having to remove the column. This application datasheet introduces the results of measurements of vitamin C and reserpine, which are usually difficult to analyze with a GC.
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55% Analysis of Fatty Acids in Food Using PCIGC- MS/MS
While some fatty acids, such as the n-3 fatty acids EPA and DHA, are beneficial to human health because they lower the amount of blood-borne neutral fat, too much intake of saturated fatty acids raises the risk of some diseases. For this reason, there is a need for the batch analysis of these fatty acids in the life sciences and food engineering sectors. Despite requiring methylation, GC-MS has gained attention because of its suitability for multicomponent batch analyses.
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55% Determination of Fatty-acid Composition of Lipids in Human Embryonic Stem Cells Using GC-MS
The cellular lipids were extracted from frozen pellets of human ES (embryonic stem) cells, and the constituent fatty acids were esterified to form fatty acid methyl esters. GC/MS analysis of the methylated fatty acids resulted in identification of 19 types of fatty acids. This report introduces the pretreatment protocol used to extract the lipids from the cells, and the fatty acids that can be analyzed by this process.
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55% Easy Screening for Residual Pesticides in Processed Foods Using GC-MS/MS
This data sheet reports on the results of applying Quick-DB and the Twin Line MS system to the analysis of residual pesticides in curry. Please also refer to Application Data Sheets No. 91 and No. 92. Application Data Sheet No. 91 introduced an example of easy screening for residual pesticides in foods using GCMS, while No. 92 introduced an example of using two columns with different separation patterns for easy screening of residual pesticides in foods.
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55% Analysis of Blood Alcohol by Headspace with Simultaneous GC-FID and MS Detection
Determination of Blood Alcohol Content (BAC) has been a standard analytical method in criminal labs for many years. The typical instrument configuration consists of a static headspace instrument for sample introduction, followed by gas chromatography (GC) with two dissimilar capillary columns for separation, and two Flame Ionization Detectors (FIDs) for detection and quantitation. Two sets of data are obtained simultaneously, and the quantitative results from the two FIDs are compared for confirmation of the reported BAC levels.
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55% Analysis of Brominated Flame Retardants and Phthalate Esters In Polymers Under the Same Conditions Using a Pyrolysis GC-MS System (2): Phthalate Esters
In recent years, an analysis method is required to determine not only polybrominated biphenyls (PBBs) and polybrominated diphenyl ethers (PBDEs), which are regulated under the RoHS Directive, but also phthalate esters and other brominated flame retardants not governed by the directive (such as tetrabromobisphenol A, hexabromocyclododecane, and bis(pentabromophenyl)ethane). Diisobutyl phthalate (DIBP), di-n-butyl phthalate (DBP), benzyl butyl phthalate (BBP), and bis(2-ehtylhexyl) phthalate (DEHP) are specified in the REACH SVHC (Substance of Very High Concern) list. This Application Data Sheet shows the results from analyzing seven phthalate esters in polymers under the same analytical conditions as those in Application Data Sheet 47 using EGA/PY-3030D Multi-Shot Pyrolyzer and GCMS-QP2020 Ultra systems.
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54% Analysis of Dietary Supplements
Analytical Instrumentation & Software for the Evaluation, Identification and QA/QC of Dietary Supplements
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54% Polymer Analysis Using TGA-GC/MS with CI
In mass analysis, a sample is ionized, the mass numbers are measured and qualitative analysis is performed on the basis of the obtained mass spectra. The ionization methods include electronic impact ionization (EI) and chemical ionization (CI).
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54% GCxGC Handbook: Application Compendium of Comprehensive 2D GC Vol. 1-5 (Japan)
GCxGC Handbook: Application Compendium of Comprehensive 2D GC Vol. 1-5 (Japan)
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54% Simultaneous Analysis of Residual Pesticides in Foods via the QuEChERS Method Utilizing GC-MS/MS
This application demonstrates the simultaneous analysis of residual pesticides in foods using the QuEChERS method.
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54% Scan/MRM Analysis of Residual Pesticides in Foods Using GC-MS/MS (3)
This application presents an investigation of residual pesticides in food using the Scan/MRM mode
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49% Development of Green Technologies in GCMS-QP2010 Ultra
There has been an increasing interest in reducing running costs and environmental stress in many analytical laboratories using gas chromatograph-mass spectrometers in the fields of food, forensic, life science, environment, and the like. To respond such interest, we have developed two technologies for lower power and carrier gas consumption: “Advanced Scanning Speed Protocol” (ASSPTM) necessary for the Fast-GC/MS method and “Ecology Mode” for saving energy in analysis standby mode. The analysis of organic acids in urine for evaluation showed that the use of the Fast-GC/MS with ASSPTM reduced helium gas by 90 percent and power by 66 percent, compared with our previous model, while retaining performance for analysis. An evaluation test in analysis standby mode confirmed that the instrument with the Ecology Mode reduced carrier gas and power consumption, by 60 percent and 36.5 percent, respectively. These results demonstrate that our GCMS-QP2010 Ultra has environmentally friendly features and ensures lower running costs.
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49% Characterization of Paint from Art Work Using Automatic Reactive Pyrolysis in the OPTIC-4 Inlet
Pyrolysis GC-MS provides an extremely useful method for art research, because it allows solid samples to be directly analyzed and requires only small sample quantities. However, pyrolysis of natural polar substances can generate polar compounds that cannot be measured by GC. To overcome this limitation, the thermally assisted hydrolysis and methylation-gas chromatography (THM-GC) method can be used. This method converts polar macromolecules into weakly polar hydrolyzed and methylated degradation compounds. The information obtained from THM-GC is useful for identifying the composition of paints, varnishes, coatings, or binders (natural) in art work. However, performing THM-GC methods using a Curie-point or filament type pyrolyzer can result in problems with repeatability or quantitative analysis. In contract, the OPTIC-4 enables performing all THM reactions within the inlet. Therefore, by optimizing the TMH conditions, it enables obtaining good repeatability. This datasheet presents the results from applying the THM-GC/MS method to characterize complicated samples characteristic to paint from air work.
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49% Effectiveness of Fast Scanning Measurement Using GC×GC-MS
A GC×GC-MS system incorporates 2 chromatographic columns with different separation characteristics, non-polar and polar columns, permitting acquisition of comprehensive 2-dimensional chromatograms by orthogonal separation according to boiling point and polarity. Excellent separation is obtained with an extremely narrow chromatographic peak width on the order of hundreds of milliseconds. Even compounds that are difficult to separate by conventional GC-MS can now be separated using GC×GC-MS. It is an extremely effective system for qualitative analysis, which requires the separation of target substances in complex matrices, common in natural substances, as well as impurity analysis. However, due to the requirement for MS capable of very fast scan speed, quadrupole MS systems have been considered to be unsuitable for GC×GC-MS. The GCMS-QP2010 Ultra resolves this problem with a scan speed as high as 20,000 u/sec.
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49% Screening of residual pesticides in food with two different columns
In recent years, with increases in the number of pesticides and the diversification of substances under investigation, there have been calls for quick and high-accuracy screening for residual pesticides in foods using GC-MS. The Quick-DB database includes mass spectra, retention times, and calibration curves for 478 pesticide components. It can be used to quickly calculate semi-quantitative values without requiring analytical standards. If a pesticide peak is detected, it is essential to check for potential interference from co-eluting contaminants to insure highly accurate screening. One of the ways for checking is to analyze the samples with two columns with different types of stationary phase. For this purpose, the Twin Line MS system is very useful because it enables the installation of two types of columns to one MS. The Quick-DB contains information compatible with two different columns, so that it can also be applied to the Twin Line MS system. In this Data Sheet, residual pesticides in foods were analyzed by applying Quick-DB and the Twin Line MS system.
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49% Effectiveness of Metabolomics Research Using Gas Chromatograph / Quadrupole Mass Spectrometer with High-Sensitivity and High-Speed Scanning
Gas Chromatography/Mass Spectrometry (GC/MS) has been recognized as a core technology for metabolomics research for the comprehensive analysis of low-molecular weight compounds in living organisms, and is widely used for biomarker discovery and quality assessment. To fulfill the demand for a high-throughput, high-sensitivity analytical system for GC/MS metabolomics research, we developed a high-speed quadrupole mass spectrometer (Q/MS), and conducted studies to determine the effectiveness of this instrument. In an experiment using a standard sample consisting of 10 amino acids, acquisition was possible over a wide quantitation range of 3.5–4.5 orders of magnitude. In analysis of the plasma from a mouse with acute inflammation, identification and quantitation of 168 compounds were possible, and using common inflammation markers, we were able to characterize the convergence process of the acute inflammatory response, which previously was not possible from the perspective of the metabolome. With the advent of this GC-Q/MS, further development in the study of metabolomics can be expected.
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47% Analysis of PCBs and Organochlorinated Pesticides in River Water Using Simultaneous Scan/MRM Measurement in GC/MS/MS (1)
This Application Data Sheet reports on MRM sensitivity and analytical repeatability when using simultaneous scan/MRM measurements with respect to PCBs and chlorinated pesticides. Application Data Sheet No. 79 presents an example of the quantification of PCBs and chlorinated pesticides by using the MRM data acquired in simultaneous scan/MRM measurements of a river water sample. In addition, Application Data Sheet No. 80 features an example of screening for environmental pollutants not suited to MRM measurement by applying Compound Composer Database Software to the scan data acquired in simultaneous scan/MRM measurements.
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47% Analysis of PCBs and Organochlorinated Pesticides in River Water Using Simultaneous Scan/MRM Measurement in GC/MS/MS (2)
This data sheet introduces an example of the accurate quantification of PCBs and chlorinated pesticides in river water using the analytical conditions for simultaneous scan/MRM measurements introduced in Application Data Sheet No. 78. It presents sensitivity and repeatability results for PCBs and chlorinated pesticides in MRM mode, acquired with simultaneous scan/MRM measurements, in Application Data Sheet No. 78. In addition, Application Data Sheet No. 80 introduces an example of the screening for environmental pollutants not suited to MRM measurement by applying Compound Composer Database Software to the scan data acquired with simultaneous scan/MRM measurements.
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47% Analysis of PCBs and Organochlorinated Pesticides in River Water Using Simultaneous Scan/MRM Measurement in GC/MS/MS (3)
This Application Data Sheet introduces an example of screening for environmental pollutants other than PCBs and chlorinated pesticides, which are targeted for MRM measurements, by applying Compound Composer Database Software to the scan data acquired in simultaneous scan/MRM measurements. Also introduced is an accurate trace quantitation analysis of PCBs and chlorinated pesticides with the MRM data acquired in simultaneous scan/MRM measurements in Application Data Sheets No. 78 and No. 79.
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47% Quantitative Determination of Volatile Organic Compounds in Drinking Water by EPA Method with Headspace Trap GC-MS
This application shows that the HS-20 Trap mode is a viable alternative method in analysis of VOCs following EPA 524.3. The correlation coefficient, recovery and RSD obtained in this study complies with EPA Method 524.3. This application shows that the HS-20 Trap mode is a viable alternative method in analysis of VOCs following EPA 524.3. The correlation coefficient, recovery and RSD obtained in this study complies with EPA Method 524.3.
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47% Analysis of Metabolites in Rat Urine Using MRM via GC-MS/MS
GC#27;MS provides excellent chromatographic separation, and is capable of highly sensitive and stable measurements. Consequently, GC#27;MS is widely utilized when measuring low#27;molecular#27;weight metabolites in living organisms and performing metabolomicanalysis to assess their fluctuations. When analyzing biological samples featuring a large number of matrix components, however, separation from the matrix can be difficult using GC#27;MS. An alternative is GC#27;MS/MS, which provides excellent separation performance via MS. In this Application Datasheet, an analysis of metabolites in rat urine is performed using GC#27;MS/MS. Results comparing this data with GC#27;MS data are also introduced.
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47% Analysis of PBDEs in Sediment Using GC-MS/MS
Polybrominated diphenyl ethers (PBDEs) are brominated flame retardants (BFRs) widely used in plastics. Due to their toxicity and persistence, their industrial production is to be eliminated under the Stockholm Convention. Contamination from PBDEs is widespread in the environment, including in sediments. In order to determine PBDEs in sediments, they need to be separated from a large amount of interferences co-extracted during sample preparation. GC-MS and GC-MS/MS were evaluated for this purpose.
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47% Analysis of Residual Pesticides in Processed Foods Using GC-MS/MS
This application datasheet presents a study of an analysis of residual pesticides in retort-pouched curry (curry packaged in a sealed pouch) using QuEChERS and GC-MS/MS. QuEChERS method is commonly used for extraction and cleanup of food products prior to GC/MS/MS analysis, due to its speed and simplicity, while GCMS/MS is able to selectively separate residual pesticides from a large quantity of co-extracted substances.
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47% GC-MS/MS analysis of residual pesticides in spinach using Scan/MRM (2)
The triple-quad GCMS-TQ8030 is equipped with a scan/MRM mode that allows simultaneous full-scan and MRM data measurements. With this mode, target compounds are selectively determined from a MRM data and unknown compounds are identified from a full-scan data, respectively. This Application Data Sheet demonstrates the effectiveness of the scan/MRM mode when analyzing residual pesticides in a vegetable after QuEChERS extraction.
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47% GC-MS/MS analysis of residual pesticides in spinach using Scan/MRM
The triple-quad GCMS-TQ8030 is equipped with a scan/MRM mode that allows simultaneous full-scan and MRM data measurements. With this mode, target compounds are selectively determined from a MRM data and unknown compounds are identified from a full-scan data, respectively. This Application Data Sheet demonstrates the effectiveness of the scan/MRM mode when analyzing residual pesticides in a vegetable after QuEChERS extraction.
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47% GC-MS/MS Analysis of Pesticides in Drinking Water
According to Japan’s list of drinking water quality control substances, pesticides are included as supplemental items subject to analysis. Designed to complement the standards, the Ministry of Health, Labour and Welfare encourages water utilities to monitor pesticide levels and achieve specified targets. Among the 102 listed pesticides, 84 are simultaneously analyzed using solid-phase extraction and GC-MS. In this datasheet, those pesticides were analyzed using GC-MS/MS and Multiple Reaction Monitoring (MRM) mode.
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47% Analysis of Metabolites in Rat Urine Using Scan/MRM via GC-MS/MS (2)
The GCMS-TQ8030 is a GC-MS/MS system equipped with Scan/MRM mode to allow simultaneous Scan and MRM data measurements. Using Scan/MRM mode, it is possible to perform high-sensitivity analysis through high selectivity via MRM, while simultaneously performing qualitative analysis utilizing the mass spectrum via Scan.
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47% Analysis of Dibutyltin Compounds in Polyvinyl Chloride Products by GC/MS
Organotin compounds are used widely throughout the world in pesticides, fungicides, wood preservatives, and adhesives. Since organotin compounds are also added to plastics such as polyvinyl chloride (PVC) for thermal stabilization and stabilization against catalytic activity, dibutyltin (DBT) compounds in PVC are now subject to a standard limit established in Japan's Food Sanitation Act. In the material testing1) of this material in its food packaging form, the DBT peak elution time and area value in the test solution and standard solution are compared, and the peak area in the test solution must be smaller than that in the standard solution (concentration of 50 μg/g or less in sample; corresponding to a concentration of 1 μg/mL standard solution). In this Application News, we introduce an example of analysis of DBT compounds in a PVC product.
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47% Search of Polymer Additives Using Mass Spectral Libraries
Commercial MS libraries can be used to identify compounds detected using GC/MS. Such MS libraries include the NIST spectral library (spectra for 160,000 compounds) and the Wiley spectral library (310,000 spectra). In addition, a specific MS library (ADD-MS06 Library: Frontier Laboratories Ltd.) has recently been developed for polymer additives. This library consists of the mass spectra and retention indices (RI) of the main peaks and pyrograms observed following Pyrolysis (Py)-GC/MS (pyrolysis at 600°C) of the 329 main commercial organic additives. This Application News introduces an investigation using the abovementioned three libraries to identify the additives contained in various polymers.
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47% Higher Sensitivity Analysis of 2-Methoxy-3-Isobutylpyrazine (MIBP) in Wine Using GC-MS/MS(PCI)
2-Methoxy-3- isobutylpyrazine (MIBP) is an aromatic substance with the fragrance of bell peppers. It is found in sauvignon blanc (a type of grape used for white wine) and cabernet sauvignon (a type of grape used for red wine), and gives the wines a favorable aroma. MIBP, which has a significant impact on the flavor of wine, has an extremely low threshold value in sensory tests, on the order of a few ng/L. Since wine contains many components, concentration and selective separation & detection are essential to analysis.
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45% GC-MS/MS Analysis of PAH and PCB in environmental samples
The polychlorinated biphenyls (PCB) and polyaromatic hydrocarbons (PAH) are among the most commonly analysed compound classes in environmental analysis. This article demonstrates how hydrogen carrier gas can be used in place of helium for GC-MS/MS analysis of these compounds.
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45% Analysis of Metabolites in Rat Urine Using Scan/MRM via GC-MS/MS (2)
This Application Datasheet introduces the results of Scan/MRM measurements of metabolites extracted from rat urine. Five target components are measured with MRM, and unknown peaks are identified through a library search of the mass spectrum obtained with Scan.
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45% Analysis of VOCs in Water Using Headspace-GC/MS
Volatile Organic Compounds (VOCs) in water are regulated by environmental water quality laws or standards because of health hazard factors. Purge and trap methods and Headspace methods are commonly used for analysis of VOCs because of their sensitivity and ease of use. This application presents an example of analysis of VOCs in drinking water using the Shimadzu HS-20 headspace sampler.
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45% Analysis of Rubber by Pyrolysis-GC/MS -Introduction of Detector Splitting System-
Here we introduce an example of analysis focusing on sulfur compounds in rubber by pyrolysis-GC/MS using a system in which a mass spectrometer (MS) is connected as detector 1, and the high sensitivity sulfur detector, FPD (S-mode), is connected as detector 2.
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45% Ultra-Fast Analysis of Volatile Organic Compounds in Water By Headspace-GC/MS/MS
This study presents analysis conditions for 25 VOCs covering a range of volatility, using static headspace as the sample introduction technique, with GC/MS/MS operated in the MRM mode for detection and quantitation. Use of a short, narrow-bore capillary column permitted analysis of 8 samples per hour, or approximately one sample every 7 minutes.
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37% New Applications for Multidimensional Gas Chromatography
Multidimensional chromatography is the utilization of multiple columns to enhance difficult separations. For a complex mixture there may not be a single column that resolves all the peaks of interest. A better separation may be possible using two columns of different polarity. In this poster, analysis examples using the MDGC/GCMS system, which utilizes a multi-Deans switch, are presented.
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37% Proposal to Reinforce Forensic Laboratories
In scientific criminal search and study, miscellaneous techniques are used for ultra high
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36% Selective Detection Using Neutral Loss Scan in GC-MS/MS
Neutral loss scan is a measuring mode of GC-MS/MS and is used to study a class of compounds showing a common neutral molecule loss during CID (Collision-Induced Dissociation). Both Q1 and Q3 are scanned with a fixed m/z difference corresponding to the neutral fragment during the CID. This application datasheet presents the results from measuring PCBs in transformer oil by neutral loss scanning.
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36% Analysis of Psychotropic Drugs in Whole Blood Utilizing Simultaneous Scan/MRM Measurements (2)
This Application Data Sheet introduces the application of scan data from simultaneous scan/MRM measurement to the Forensic Toxicological Database, leading to comprehensive screening for medicinal toxicants in a whole blood sample. Please refer to this in conjunction with Application Datasheet No. 74, which introduces an example of the mass separation of cholesterol from triazolam and etizolam in whole blood, utilizing MRM data from simultaneous Scan/MRM analysis.
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36% Analysis of Pesticides in Food Matrix using QuEChERS by Triple Quadrupole GC/MS/MS and LC/MS/MS
Inspection results for 138 pesticides using GC/MS/MS and LC/MS/MS have been reported by the EURL (European Union Reference Laboratory). Of the 138 substances, it was recommended that 66 of those substances be analyzed using the triple quadrupole GC/MS/MS, and that the remaining 72 substances be analyzed by the triple quadrupole LC/MS/MS. Thus, the pesticides were analyzed using the GCMSTQ8030 and LCMS-8040 as recommended. The combined use of GC/MS/MS and LC/MS/MS permits high-sensitivity comprehensive analysis of residual pesticides in foods.
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36% Analysis of MOSH and MOAH using SPE prior to GC×GC-MS analysis
The present work is focused on the development/optimization of a comprehensive two-dimensional gas chromatography method, with dual detection [flame ionization (FID) and mass spectrometric], for the simultaneous identification and quantification of mineral-oil contaminants in a variety of food products. The two main classes of contaminants, namely saturated and aromatic hydrocarbons, were previously fractionated on a manually-packed silver silica solid-phase extraction (SPE) cartridge. The presence of a series of unknown compounds was investigated using the mass spectrometric data, and were tentatively-identified as esterified fatty acids, most probably derived from vegetable oil based ink.
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36% Systematic screening for basic and acidic drugs in urine using Ultrafast-GCMSMS in MRM-scan mode – Part 1
We report a GC-EI-MSMS method using MRMs for the detection of targeted compounds, the unequivocal confirmation of structure using EI-MS and a background full scan that is useful in detecting the presence of unexpected substances and changes to the normal sample matrix. The method is enabled by the use of a UFMS capable GCMSMS system that is capable of supporting simultaneous MRM-scan-PIS.
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35% Analysis of a Gas Emitted from Polystyrene Using the Thermal Desorption Method
Generally, in analyzing gases emitted from a sample, the sample is first loaded into a chamber and Tedlar bag, where it is sealed and then heated. Afterwards, the gas emitted is concentrated in a collection tube, and then analyzed using the thermal desorption method. With the TD-20 thermal desorption system, the sample is loaded directly into a glass tube exclusively designed for the TD-20 (P/N: 223-57119), and the gas emitted when the sample is heated can be analyzed without utilizing a chamber. This not only reduces the amount of work required when sampling, but also reduces the loss of high boiling-point substances because the flow line has been significantly shortened. In this experiment, 0.5 mg of polystyrene was loaded into a glass tube for the TD-20. Both ends of the tube were fastened with 5 mg of quartz wool. The gas emitted when the sample was heated at 250oC for 30 minutes was then analyzed, and the total ion current chromatogram (TIC) obtained is shown below. It can be confirmed that, in addition to the polystyrene monomer styrene, dimer, trimers, and tretramers were produced when the sample was heated at 250 ℃.
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32% Analysis of Residual Solvents – Class 1, Class 2A, Class 2B – in Pharmaceuticals Using Headspace-GC/MS
Residual solvents in pharmaceuticals are defined as organic volatile chemicals that are used or produced in the manufacture of drug substances or excipients, or in the preparation of drug products. Residual solvents are classified and managed as Class 1 to Class 3 substances, depending on the risk they pose to human health. According to the USP, "General Chapter <467> Residual Solvents" method, analysis of these residual solvents is to be conducted by the headspace GC-FID method (HS-GC). However in this application, we investigated using headspace-GC/MS (HS-GC/MS) according to Procedure A. Using the HS-GC method, measurement is to be performed as three separate analyses, which are required to achieve chromatographic separation within the three different classes of compounds, Class 1, Class 2A, and Class 2B. However, using HS-GC/MS, complete chromatographic separation is not necessary, so all the compounds can be analyzed in a single run. In addition, compound confirmation and qualitative information of unknown peaks can also be obtained.
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26% Understanding RoHS What it Means to U.S. Manufacturers
With the Restriction of Hazardous Substances (RoHS) regulations scheduled to take effect in July 2006, there is ongoing concern regarding the readiness of American companies to meet new European standards. Initiated by the European Union, RoHS is a direct response to growing calls for the regulation of materials deemed hazardous (including lead, cadmium, and mercury) in consumer electronic equipment.
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