LITERATURE


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75% All roads lead to Rome - Fast LC -How
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74% Fast LC Analysis of Soft Drinks
This application note details the separation and detection of five beverage compounds with fast LC gradient conditions.
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63% LC-10AS Pump Recommended Spare Parts List (LC-014)
The following is a list of recommended spare parts for the LC-10AS Pump. This list encompasses those consumable items which are most frequently replaced.
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63% LC-10AD Pump Recommended Spare Parts List (LC-015)
The following is a list of recommended spare parts for the LC-10AD Pump. This list encompasses those consumable items which are most frequently replaced.
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63% High-Speed, High-Resolution Analytical Applications (Part 9) Simultaneous Determination of Tryptophan Metabolites
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63% Application of High-Speed, High-Resolution Analysis (Part 5) Analysis of Artificial Colorings
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63% Application of High-Speed, High-Resolution Analysis (Part 4) Analysis of Cephem Antibiotics
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63% Application of High-Speed, High-Resolution Analysis (Part 3) Analysis of Isothiocyanates and Sinignin
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63% Analysis of Natamycin in Cheese
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63% Analysis of α-Lipoic Acid in Dietary Supplement
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63% Analysis of N-Methylcarbamate Pesticides in Foods
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63% High Speed, High Resolution Analytical Applications (Part 2) Analysis of Isoflavones in Soy
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63% Determination of Cyanidin-3-Glucoside in Black Soybeans
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63% Determination of Polyether Antibiotics in Animal Feeds (Part 2) Analysis using Fluorometric Detection
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63% Determination of Quinoxaline-2-Carboxylic Acid in Pork
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63% GPC Cleanup Methods in Pesticides Residue Analysis of Food(Part2)
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63% Analysis of Capsaicinoids in Spices
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63% Analysis of Hexavalent Chromium by Post-column Derivatization Method
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63% Analysis of Impurities in Drugs by Prominence Series UV-VIS Detectors (Part 2)
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63% Analysis of Sudan Dyes in Foods
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63% Analysis of Canthaxanthin and Astaxanthin
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63% Analysis of Malachite Green in Cultured Fish (Part 2) Simultaneous Analysis with Leucomalachite Green
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63% Determination of Coenzyme Q10 in Food
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63% Determination of Drugs in Blood Plasma by “Co-Sense for BA” (Part 7)
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63% Analysis of Impurities in Drugs by Prominence Series UV-VIS Detectors
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63% Analysis of Bromate by Newly Developed Shim-pack IC-Bromate Column
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63% Analysis of Malachite Green in Cultured Fish
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63% Analysis of Amino Acids in Vinegar
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63% Analysis of Hyaluronic Acid
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63% Analysis of Lycopene and β-Carotene in Tomato
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63% Determination of Benzoyl Peroxide in Foods
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63% Analysis of Tricyclic Antidepressants
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63% Determination of Drugs in Blood Plasma by “Co-Sense for BA” (Part 6)
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63% Analysis of Enrofloxacin in Broiled Eels
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63% Analysis of Glyphosate in Drinking Water by HPLC
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63% Analysis of Iprodione, Asulam, Thiophanate-methyl, and Siduron in Drinking Water by HPLC
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63% Analysis of Diquat in Drinking Water by HPLC
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63% Analysis of NO2-N in Drinking Water by Ion Chromatography
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63% Analysis of Chlorogenic Acid in Coffee
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63% Determination of Catechins in Tea
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63% Analysis of Anionic Surfactants by HPLC in Accordance with the Revised Water Quality Testing Method
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63% Application of Evaporative Light Scattering Detector ELSD-LT (Part 3) Analysis of Surfactants
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63% Determination of Sucralose in Soft Drinks
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63% Analysis of Pesticides Residues by HPLC
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63% Analysis of Voglibose by Post-column Fluorometric Detection Method
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63% Analysis of Potassium Bromate in Bread
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63% Analysis of Bromate Ions in Drinking Water
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63% Analysis of Animal Drug (2)
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63% Shim-pack HT-ODS ・New High Throughput HPLC Column
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63% Analysis of Sulfurous Acid Ion
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63% Study of Analytical Conditions for Aldehydes in Ambient Air
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63% Analysis of Bisphenol A using High-Performance Liquid Chromatography
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63% Determination of Fumonisins in Corn
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63% Determination of Formaldehyde and Acetaldehyde in Environmental Air
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63% New Standard ODS Column “Shim-pack VP-ODS” Supports
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63% Determination of Microcystins in Blue-green Algae in Lake Water
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63% Analysis of Chlorophyll (a) in Seawater
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63% Advanced Method Development, overnight
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63% Tested and approved!
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63% Good day, sunshine
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63% Full Automation with Integrated LC
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56% LC-8A Consumable Parts List
This file contains descriptions, part numbers and comments.
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56% Fractionation by Preparative LC-MS and Fraction Verification byUltra Fast LC-MS
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56% PUMPS: Pulse-Free, Unsurpassed in Reliability, Minimal Maintenance, Proven Precision
Shimadzu’s LC-10AS, LC-10AT, and LC-10AD are pulse-free, unsurpassed in reliability, require minimal maintenance, have proven precision and are suitable for your most sensitive applications.
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56% LC-10A Series Detectors Recommended Spare Parts List (LC-018)
The following is a list of recommended spare parts for the SPD-10A/V UV/Vis Absorbance Detector and the RF-10A Fluorescence Detector. This list encompasses those consumable items which are most frequently replaced.
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56% LC-10AT Pump Recommended Spare Parts List
The following is a list of recommended spare parts for the LC-10AT Pump. This list encompasses those consumable items which are most frequently replaced.
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56% LC-10Ai Pump Recommended Spare Parts List
The following is a list of recommended spare parts for the LC-10Ai Pump. This list encompasses those consumable items which are most frequently replaced.
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56% Reducing Analysis Limitations by Using Multiple Detectors with Ultra Fast LC
Reducing Analysis Limitations by Using Multiple Detectors with Ultra Fast LC
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55% HPLC Detection of Polynuclear Aromatic Hydrocarbons
The polynuclear aromatic hydrocarbons are a ubiquitous class of highly lipophilic, potentially carcinogenic substances consisting of a large number of individual compounds containing aromatic rings.
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55% Analysis of Biofuel (Part 2) Determination of Formic Acid, Acetic Acid, and Propionic Acid in Biodiesel - Diesel Fuel Blend
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55% Analysis of Biofuel (Part 1) Determination of Fatty Acid Methyl Esters and Triglycerides in Biodiesel Blended Fuel
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55% High Speed, High Resolution Analytical Applications (Part 1)Introducing Newly-Developed “Shim-pack XR-ODS” Column
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55% Determination of Polyether Antibiotics in Animal Feeds (Part 1) Analysis by Post-column VIS Photometric Derivatization Method
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55% Analysis of ClO2 -, ClO3 -, and ClO2 in Tap Water by Ion Chromatography
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55% Direct Injection of Blood Plasma for the Determination of Drugs using “Co-Sense for BA” (Part 5)
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55% Direct Injection of Blood Plasma for the Determination of Drugs using “Co-Sense for BA” (Part 4)
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55% Napoleon did not die from arsenic poisoning - ADAIS- Automated poison and drug analysis
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55% LC Separations under 100% Aqueous Conditions using Pathfinder Columns
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55% Analysis of Barbiturates by UFLC
This application note details the separation and detection of seven barbiturates with ultra-fast LC (UFLC) conditions.
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55% Evaluation of the Current Capabilities of Ultra High Pressure LC and High Temperature LC
UHPLC is becoming an increasingly popular technique for sample analysis due to the ability to reduce analysis times or improve resolution. However, the use of ultrahigh pressures can reduce column lifetime and increase the maintenance requirements of the LC system. Another less commonly used technique to reduce analysis times is the use of high-temperature LC. High-temperature LC has the advantage of reducing system pressure by lowering solvent viscosity and may provide the ability to reduce the organic content of the mobile phase. The use of high-temperature LC has been limited by the number of available columns for this technique. A number of applications will be presented up to 19,000 psi and 150 degrees C for the technique.
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53% Reduced Solvent Consumption and Operational Costs with Existing LC Systems
Although the introduction of ultra-high speed LC is an effective way to conserve solvent, there is a very significant issue as to how this can be achieved with the existing LC systems in use. This report introduces detailed suggestions on how to most effectively reduce solvent consumption with existing LC systems.
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52% LC8A Pump Provides Analytical-to-Prep Scale-Up
The LC8A Pump Provides Analytical-to-Prep Scale-Up, is versatile and easy to use, and offers single point control from system controller or CLASS-VP software.
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52% Determination of Stavudine Utilizing Semi-Microbore Electrospray LC/MS
It is generally accepted that sensitivity of electrospray ionization LC/MS (ESI) isconcentration dependant, i.e., overall method sensitivity can be enhanced by scaling down the column diameter,flow rate and amount of sample injected on-column.
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50% LCMS-2020 - Reduces Limitations of HPLC Analysis and UFLC Analysis
As explained above, building an LC/MS system that employs an MS as an LC detector can effectively reduce the limitations of LC analysis. In addition to this, reducing those limitations to the greatest extent possible requires an MS that can handle ultra high speed analysis. The new “LCMS-2020” single quadrupole ultra-fast LC/MS, boldly addresses these requirements.
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49% On-Column Detection Limit for Testosterone by APCI LC/MS
This brief investigation demonstrates the ability of the Shimadzu LCMS QP-8000 for the rapid development of a high sensitivity method for testosterone.
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49% Fraction Collection Chromatography Application: Handy Scaled-Up Method using LC-8A
Recently, preparative chromatography has been widely used to purify synthetic reagents and extracts, and for structural analysis. The column volumes required for preparative chromatography, however, range from 10 to 100 times those of conventional LC columns. The absolute injection amounts for preparative chromatography can range from 10(3) to 10(6) times those of conventional LC analysis. This sometimes makes preparative chromatography difficult to directly implement without changing the original analysis conditions. A simple upgrade, using the Shimadzu LC8A, circumvents this implementation difficulty.
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49% Analysis of Bromate by Post-column Ion Chromatography
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49% Analysis of Cyanide Ion and Cyanogen Chloride by Post-column Ion Chromatography
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49% Determination of Alliin in Garlic
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49% Determination of Theanine in Tea
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49% Application of Evaporative Light Scattering Detector ELSD-LT (Part 2) Analysis of Oligosaccharides in Beer
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49% Direct Injection of Blood Plasma for the Determination of Drugs using “Co-Sense for BA” (Part3)
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49% Analysis of Amino Acids in Fermented Food and Drinks
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49% Application of Evaporative Light-Scattering Detector “ELSD-LT”(Part1) Analysis of Triglycerides in Cooking Oil
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49% Determination of Water-Soluble Vitamins in “Foods with Nutrient Function Claims”
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49% Determination of Hydrocarbon Types in Diesel Fuel According to the JPI* Method *(Japan Petroleum Institute)
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49% Direct Injection of Blood Plasma for the Determination of Drugs using “Co-Sense for BA” (Part 2)
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49% Direct Injection of Blood Plasma for the Determination of Drugs using “Co-Sense for BA” (Part 1)
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49% HPLC Analysis of Isocyanuric Acid and LAS Anionic Surfactant by the JWWA* Method (* Japan Water Works Association)
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49% HPLC Analysis of Carbofuran, Methomyl, and Carbaryl by the JWWA* Method (* Japan Water Works Association)
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49% Analysis of Phenolic Antioxidants in Food
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49% Rapid and Sensitive Analysis of Aluminum Ions using On-column Derivatization (Part 1)
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49% Analysis of Polyether Antibiotics in Animal Feed
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49% Analysis of antidepressants - Pathfinder HPLC column technology
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49% Analysis of antidepressants - Pathfinder HPLC column technology
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49% High-Speed Analysis of Ginsenosides
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49% High-Speed Analysis of Cortisone and Cortisol
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49% High-Speed Analysis of Aldehydes and Ketones
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49% High-Speed Analysis of Lycopene
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49% High-Speed Analysis of Furfural
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49% High Speed Analysis of Vitamin D
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49% High Speed Analysis of Phenolic Antioxidants
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49% High Speed Analysis of Organic Acids
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49% High Speed Analysis of Sennosides
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49% High Speed Analysis of Glycyrrhetinic Acid
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49% High Speed Analysis of Sulfa Drugs
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49% High Speed Analysis of Sudan Dyes
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49% High Speed Analysis of Synthetic Sweeteners
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49% High Speed Analysis of Hesperidin
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49% High Speed Analysis of Food Preservatives
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49% High Speed Analysis of Scopolamine and Hyoscyamine
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49% High Speed Analysis of Chlorfluazurons
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49% High Speed Analysis of Chlorhexidine
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49% AXIMA-TOF2 LC-MALDI Software Guide
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49% Prominence UFLC Next Generation High Throughput-HPLC
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49% Selecting Detectors for Compounds with No Optical Absorbance
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49% Prominence nano System and Applications
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47% High Speed, High Resolution Analysis (Part 15) Analysis of Ginsenosides in Ginseng
This Application News introduces an example of the analysis of ginsenosides in ginseng using the ultra fast LC system “Prominence UFLC” with the Phenomenex Synergi 2.5 μm Polar-RP high-speed, high-resolution column.
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47% Analysis of Halogens in Polyphenylene Sulfide Resin Using a Combustion-Ion Chromatography System
Here we introduce an example of analysis of halogen in a commercially available polyphenylene sulfide (PPS) resin using a combustion-ion chromatography system.
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47% SIL-10A Autosampler Recommended Spare Parts List (LC-016)
The following is a list of recommended spare parts for the SIL-10A Autosampler. This list encompasses those consumable items which are most frequently replaced.
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47% SIL-6B/9A Autosampler Recommended Spare Parts List (LC-017)
The following is a list of recommended spare parts for the SIL-6B/9A Autosampler. This list encompasses those consumable items which are most frequently replaced.
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47% High Speed Analysis of Low Level Estrogens and Their Metabolites from Biological Samples
Introducing a novel multicolumn approach that uses an ultra-high pressure LC system for Cumulative Multicolumn Sequential Chromatography with different column chemistries for the required resolution, significantly reducing analysis time.
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47% Identification of Proteins by LC-MALDI System
This application note introduces that protein identification by MS/MS ion search using an LCMALDI system which combines a nano-LC system (Prominence nano-AccuSpot-AXIMA® Performance).
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47% Analysis of Melamine and Its Related Substances in Fertilizers
This article introduces an example of the analysis of melamine and related substances in fertilizers using an HPLC system, with reference to the fertilizer test methods (2012) under the supervision of the Japan‘s Food and Agricultural Materials Inspection Center (FAMIC).
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47% Evaluation of Kinetex Column Peak Capacity Using the Shimadzu Nexera UHPLC System
Peak capacities of 200 could be obtained with a 24 second gradient runtime for the compounds that were tested. All compounds were eluted from the column in less than 15 seconds. These results demonstrate the combined benefits of high column efficiency and system performance.
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47% High Speed, High Resolution Analysis (Part 34) Analysis of Preservatives in Cosmetics by the Nexera UHPLC System
High-speed analysis of preservatives in cosmetics was previously introduced in Application News No. L375, but we have updated this application as an example of ultra-high-speed analysis with the Nexera LC system in conjunction with the Shim-pack XR-ODS III high-speed separation column (particle size 1.6 μm).
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47% High Speed, High Resolution Analysis (Part 36) Analysis of Quinolones by the Nexera UHPLC System
Many types of quinolone synthetic antibacterials are widely used for the prevention and treatment of infectious diseases in domestic animals, domestic fowl, and cultured fish. Here, we introduce an example of simultaneous analysis of a mixture of synthetic quinolone antibacterials using the Nexera ultra high performance LC system with the RF-20Axs high-sensitivity fluorescence detector.
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47% Simultaneous Execution of 3 Separations With a Single LC-10A System
The Shimadzu LC-10A System is a versatile HPLC platform that provides ease of automation and flexibility. In response to a request from a customer performing high throughput clinical applications, we were asked to perform three separate analyses with one automated system. To accomplish this task we configured a system with two automated column selection valves, three pumps and an autosampler with cooler.
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47% Separation Of Nucleotides and Xanthines using The LC-10Ai Bioinert HPLC (LC-024)
Recently, the use of bioinert high performance liquid chromatography has increased concomitantly and most likely as a result of research burgeoning in the areas of protein and peptide chemistry. The utility of these systems stems from methodology designed to preserve biological activity and molecular conformation in biological samples.
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47% Reproducible Gradients at Ultra-Low Flow Rates
A growing number of analysts are using techniques such as microbore HPLC and LC-MS which require flow rates anywhere from 10 uL/Min to 200 uL/min. Several factors must be considered when working in this range: appropriate back pressure for reliable pump performance, efficient mixing in a very limited volume, and reduction of dead volume to minimize run time and bandspreading.
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43% Measuring Samples Per USP <643> Using Shimadzu TOC-LC*H
Shimadzu’s TOC-LC*H analyzer is perfectly adapted to meet the requirements of USP Method <643>. The TOC-LC*H analyzer utilizes 680°C heat and platinum catalyst to provide a lower maintenance instrument with a detection limit of 0.0040 mg per liter.
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43% Determination of Chlorhexidine, Benzethonium, and Benzalkonium in Disinfectants
Here we introduce examples of HPLC analysis of 3 active ingredients (chlorhexidine, benzethonium, and benzalkonium) found in commercially available disinfectants.
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43% Analysis of Electrolytic Solvents for Lithium-Ion Rechargeable Battery
In this application news we introduce an example of analysis of electrolytic solvents used in lithium-ion rechargeable batteries by HPLC.
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43% Rapid and Sensitive Analysis of Aluminum Ions using On-column Derivatization (Part 2) - Application to the Analysis of luminum Ions in Total Parenteral Nutrition Solutions
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43% High-Speed Analysis of Malachite Green and Metabolites
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43% High-Speed Analysis of Pesticides in Water Quality Regulation
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43% High-Speed Analysis of Fat-Soluble Vitamins
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43% High Speed Analysis of Polynuclear Aromatic Hydrocarbons (PDA Detection)
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43% High Speed Analysis of Vitamin B12
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43% High Speed Analysis of Amino Acids by Pre-Column Derivatization (2)
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43% High Speed Analysis of Amino Acids by Pre-Column Derivatization (1)
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43% High Speed Analysis of α-Lipoic Acid
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43% High Speed Analysis of Coenzyme Q10
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43% Analysis of Water Treatment Samples
Analysis of Water Treatment Samples with Shodex Columns.
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43% Analysis of Water Treatment Samples - Ammonium
Analysis of Water Treatment Samples with Ammonium using Shodex Columns.
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43% Benzodiazepine Analysis by UFLC
This application note details the separation and detection of four benzodiazepines with ultra-fast LC (UFLC) conditions.
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43% AXIMA-TOF 2-Protein Identification of Complex Mixtures using LC-MALDI on AXIMA-TOF2
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43% AXIMA-TOF 2 Simultaneous Protein Identification and Relative Quantification of Complex Mixtures using iTRAQ. methodology, LC-MALDI and AXIMA-TOF2.
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43% AXIMA-QIT - Characterization of Complex Protein Gel Bands by Offline LC-MALDI QIT TOF MS
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43% Principles and practical applications of Shimadzu's ELSD-LTII evaporative light scattering detector
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43% Migration points to fast analysis; Optimization of the system and analysis conditions
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43% Applications of Ultra Fast HPLC
This application note focuses on the high-speed analysis of xanthine derivatives and triarylmethane ink dyes with Ultra Fast HPLC.
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43% Analysis of Steviol Glycosides in Stevia Extract
We introduce an example of analysis of the 9 major steviol glycosides in the stevia extract by reversed phase chromatography.
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42% Achieving Ultra-High-Speed Analysis with LC/MS/MS
This report addresses the problems associated with conventional LC/MS/MS systems in achieving ultra-high-speed analysis, and introduces a resolution to these problems.
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42% Determination of Serum Extracts Plus Reserpine Spikes Utilizing SPE and ESI on QP-8000
This application note describes the determination of reserpine in serumutilizing the Solid Phase Extraction (SPE) and electrospray (ESI) in LC/MS.
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42% On-Column Detection Limit for Testosterone by APLC LC/MS
Atmospheric pressure chemical ionization (APCI) LC/MS offers users a highly-sensitive and specific means for the determination of non-polar and semi-polar analytes, including steroids such as testosterone
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42% LC/MS/MS Analysis of Impurities in Active Pharmaceutical Ingredients Using the Co-Sense for Impurities System
Detection of impurities in active pharmaceutical ingredients (APIs) is often conducted using an HPLC-UV method. However, qualitative and quantitative analysis of impurities requires not only the separation of the impurities from the major component, but also separation among impurities themselves. The time and effort required to establish effective analytical conditions for this type of analysis are significant. Furthermore, the source of the impurity, whether it be the sample itself or some external factor associated with a particular lot, must also be determined. Here we demonstrate analysis of an impurity in an API using the 2-dimensional LC/MS/MS separation feature of the Co-Sense for Impurities System.
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42% Analysis of Compounds Containing a Phosphate Group Using the New MastroTM High Pressure-Resistance Stainless Steel- Free LC Column
Here, we examined the effects of different flow line materials on the peak shape of coordination compounds. Also, using a formic acid mobile phase, which is commonly used in LC/MS analysis, we examined the inhibiting effect on metal adsorption of coordination compounds that is provided with MastroTM high pressure-resistance stainless steel-free analytical column.
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41% Evaluation of MS Scanning Speeds with UHPLC Peak Widths
The trend toward high-speed LC analysis with reduced column particle sizes continues to increase in popularity. The narrow peak widths of high-speed LC have generally been handled adequately with UV detectors by increasing data acquisition rates. However, use of a MS will reduce the risk of misidentifying peaks when transferring methods from conventional to high-speed LC. Most MS instruments have had difficulty keeping up with these sharp peaks, which may have peak widths of 200 milliseconds or less. Accurate mass analysis of sharp chromatographic peaks obtained by high-speed LC requires ultra-fast MS detection capabilities. A number of high-speed separations will be evaluated using MS scanning speeds of up to 15,000 u/sec with polarity switching speeds of 15 msec.
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40% Simultaneous Analysis of Irganox Polymer Additive using LC-MS
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40% Analysis of Phenoxyproprionic Type Herbicides using LC-MS
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40% Analysis of Bisphenol A Diglycidyl Ether Using LC-MS
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40% Analysis of Microcystins Using LC-MS
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40% Drug analysis using Co-Sense for BA LC-MS system (1)
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40% Analysis of fullerenes using LC-MS
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40% Analysis of alkyl phenyl polyoxyethylene sulfonate using LC-MS
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40% Analysis of benzalkonium and polyoxyethylene lauryl ether using LC-MS
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40% Analysis of fatty acid amidopropylbetaine using LC-MS
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40% Analysis of pesticides in foods using LC-MS
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40% Analysis of alkylphenols using LC-MS
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40% Analysis of Vitamin K in food using LC-MS
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40% Analysis of a drug in plasma using ON-LINE pretreatment method to remove proteins for LC-MS
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40% Analysis of penicillin analogue using LC-MS
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40% Analysis of Bisphenol A and Nonylphenol using LC-MS
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40% Analysis of DNPH-aldehydes using LC-MS
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40% Analysis of antioxidants in drug and food using LC-MS
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40% Analysis of Proteins and Peptides using LC-MS
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40% Analysis of Sudan Dyes and Para Red using LC-MS
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40% Analysis of Steroidal Anti-Inflammatory Drugs Using LC/MS
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40% Drug Analysis Using “Co-Sense for BA” LC/MS System (2)
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40% LC-MSAnalysis of Compounds Related to Nucleic Acids
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40% Simultaneous Analysis of Pesticides in Environment Water using LC-MS
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40% Simultaneous Analysis of Pesticides using LC-MS
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40% Simultaneous Analysis of Carbamate Pesticides with LC-MS
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40% Analysis of N-nitroso-fenfluramine using LC-MS
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40% High-Speed Analysis of Glycyrrhizinate
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40% High-Speed Analysis of Berberine
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40% High-Speed Analysis of Catechins
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40% High-Speed Analysis of Curcumin
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40% High-Speed Analysis of Artificial Colorant
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40% High-Speed Drug Analysis
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40% Application using Analytical / Preparative LC-MS System (Part 1)
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40% Analysis of Perfluorochemicals (PFOA, PFOS) using LC-MS
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40% Analysis of Pesticides in Food Matrix using QuEChERS by Triple Quadrupole GC/MS/MS and LC/MS/MS
Inspection results for 138 pesticides using GC/MS/MS and LC/MS/MS have been reported by the EURL (European Union Reference Laboratory). Of the 138 substances, it was recommended that 66 of those substances be analyzed using the triple quadrupole GC/MS/MS, and that the remaining 72 substances be analyzed by the triple quadrupole LC/MS/MS. Thus, the pesticides were analyzed using the GCMSTQ8030 and LCMS-8040 as recommended. The combined use of GC/MS/MS and LC/MS/MS permits high-sensitivity comprehensive analysis of residual pesticides in foods.
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39% High Speed Analysis of Ginkgolic Acids
Ginkgo leaves contain ginkgolic acid of alkylphenols that can potentially cause allergies. Here we present an example of high speed analysis using the absorbance of ginkgolic acid and fluorescence detection. Generally, fluorescence detection has the advantages of high selectivity and less likelihood that the actual sample will be influenced by impurities.
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39% High Speed Analysis of Enrofloxacin
Enrofloxacin is a synthetic new quinolone antimicrobial agent. It is used for the treatment and prevention of pneumonia and Escherichia coli diarrhea symptoms in cows and pigs. Here we present an example of high speed analysis of enrofloxacin using fluorescence detection.
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39% High Speed, High Resolution Analysis (Part 17) Analysis of Paeoniflorin in Peony Root
This Application News focuses on paeoniflorin as one of the principle active substances in peony root, and introduces an example of analysis of paeoniflorin using the ultra high-speed LC system “Prominence UFLC” with the SPD-M20A photodiode array detector.
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39% The UHPLC with the Ultimate in Resolution
Nexera supports the use of long analytical columns thereby maximizing separation efficiency thanks to a high pressure limit of up to 130MPa. In this example, two 150 mm columns with 1.8 µm particle size were connected sequentially thereby increasing Nexera’s theoretical plate number to about 70,000.
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39% Ultra-High-Speed and Ultra-High-Resolution Analysis of Drug Analogs
As analogs in drug products often have similar structures, high resolution is demanded for their analysis. A long, sub-2μm column is effective in such cases but this requires a UHPLC system with high pressure tolerance, such as the Shimadzu Nexera (130MPapressure tolerance). This Application Data Sheet introduces the ultra-high-speed and ultra-high-resolution analysis of ketoprofen and its analogs.
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39% Ultra-High-Speed Analysis of Preservatives in Cosmetics
Parabens and other preservatives are added to cosmetics to maintain safety and quality. The simultaneous analysis of these preservatives generally takes a long time, due to the many types of preservative used. This Application Data Sheet introduces the ultra-high-speed analysis of preservatives in cosmetics using the high resolution of the Shim-pack XR-ODS III column (1.6 µm particle size) and the high pressure tolerance (130 MPa) of the Nexera.
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39% Ultrafast Analysis of Aldehydes and Ketones
This document introduces an example of ultrafast analysis performed on 13 aldehyde/ketone DNPH derivatives by gradient elution using Nexera and a Phenomenex Kinetex C18 column (particle size 2.6 µm, a core-shell column where a 0.35 µm porous membrane is combined with a 1.9 µm solid core).
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39% Ultrahigh Separation Analysis of Nucleotides
For high‐separation analysis of these components, a long column with a particulate packing material can be used. However, both the UHPLC unit and column need to have a high pressure‐resistance. This document introduces an example of ultrahigh separation analysis performed on nucleotides using Nexera, which is resistant to 130MPa, and a Shim‐pack XR‐ODS III column (particle size 2.2µm), resistant to 100MPa.
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39% Analysis of Hexafluorophosphate Ion in Electrolytic Solution for Lithium-Ion Rechargeable Batteries by Ion Chromatography
Lithium hexafluorophosphate is a common electrolyte used in rechargeable batteries. Here, we introduce an example of the analysis of hexafluorophosphate ion in the electrolytic solution of a lithium-ion rechargeable battery with the Shimadzu Prominence HIC-SP suppressor-type ion chromatograph.
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39% Analysis of the Decomposition Products of Lithium Hexafluorophosphate in the Electrolytic Solution of Lithium-Ion Rechargeable Batteries by Column-Switching Ion Chromatography
Here, we introduce an analysis of the decomposition products of lithium hexafluorophosphate used as the electrolytic solution in lithium-ion rechargeable batteries, in which we constructed a column-switching system using the Shimadzu Prominence HIC-SP ion chromatography system.
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39% Separation of Tryptic Digest of Cytochrome C Using LC-7A Liquid Chromatograph
The chromatographic analysis of the tryptic digest of cytochrome C, along with many biological separations, pose special problems for standard HPLC systems. Typically mobile phases containing high salt concentrations (e.g. 1 M NaCl), or other corrosive agents such as trifluoroacetic acid, are used which will degrade the stainless steel in HPLC pumps unless the system is carefully passivated and rigorously maintained.
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39% The Analysis of Analgesics by UV Detection
In the pharmaceutical industry, different chromatographic techniques are used in method development and drug analysis. HPLC is the most common analytical technique used in the analysis of many compounds including Analgesics, Antiarrhythmics, Antibacterials, Cardiac drugs, Cholesterol-Reducing drugs, and Vitamins. The Shimadzu LC-10A Series HPLC System provides the sensitivity, reliability and precision required for pharmaceutical analyses
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39% High Sensitivity Porphyrin Analysis by Fluorescence Detection using R928 PMT
The analysis and quantitation of porphyrins for diagnosis has been, until recently, very complex and time consuming. HPLC has become a very effective analytical method, since it can separate and quantitate individual porphyrins very rapidly. The Shimadzu LC-10A System with the RF-10A Fluorescence Detector offers both high sensitivity and high selectivity in the analysis of porphyrins. The sensitivity is enhanced by using the optional Photomultiplier Tube R928-08.
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39% Analysis of Clean In Place 150 Residues on Process Equipment Using UV Detection
The pharmaceutical industry has recently become concerned with the efficacy of cleaning procedures not only to remove active drug, but also to remove the products used to clean the process equipment. At Schein Pharmaceutical in Carmel, New York, the robotics department has developed a comprehensive program to validate the efficacy of current cleaning protocols. This program incorporates the use of a Shimadzu LC-10A Series HPLC System to detect trace amounts of cleaning agents
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39% SIL-10AXL Autosampler Semi-Micro Kit (LC-031)
The utility and demand for semi microbore HPLC has dramatically increased for a variety of reasons: semi-microbore applications offer increased sensitivity, reduced sample consumption and a substantial reduction in liquid waste. Shimadzu now offers the option of implementing a semi-micro kit to easily and economically convert the SIL-10AXL analytical autosampler to an autosampler capable of semi microbore applications.
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39% Analysis of Additives in Polymers Using Fraction Collection LC-FTIR
Infrared spectroscopy is an excellent method for identification of compounds. However, when the absorbance of components overlap, it is more difficult to identify each component. In this type of situation, it is more necessary to combine this method with a separation method such as gas chromatography (GC) or liquid chromatography (LC).
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39% Evaluation of UHPLC System Performance Under High Throughput Conditions
The use of UHPLC technology has continued to increase in LC applications as a way to reduce sample analysis turnaround times. A variety of applications in pharmaceutical and environmental areas with small particle columns will be presented that will demonstrate the throughput and performance that can be obtained with current UHPLC technology.
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39% Analysis of Minor Components in Synthetic Polymers Using SEC-AccuSpot-AXIMA System (1)
Shimadzu has developed the AccuSpot automated spotter to mix the eluate from the LC with the various reagents and directly load them onto the MALDI sample plate. As a result, an automated analytical SEC-MALDI analysis system was constructed that can rapidly simplify all the processing from LC separation to MALDI-TOFMS measurement.
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39% Open Solution: Open Access Environment by Web Browser
As described above, there are great advantages to be realized in an open access system comprising a closed network in a laboratory that is linked to an open network in a separate office environment. The introduction of Open Solution provides an open access environment which can be utilized to its maximum to achieve great efficiencies in LC and LC/MS analysis in the analytical laboratory.
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39% Evaluation of MS Scanning Speeds with Ultra Fast Liquid Chromatography
Accurate mass analysis of sharp chromatographic peaks obtained by high-speed LC requires ultra-fast MS detection capabilities. In this poster, a number of high-speed separations are evaluated with MS scanning speeds of up to 15,000 u/sec with polarity switching speeds of 15 msec.
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39% High Speed, High Resolution Analysis (Part 39) Analysis of Aflatoxins in Food by Nexer
Here we introduce an example of ultra-high-speed analysis of aflatoxins in food using a combination of the Prominence RF-20Axs high-sensitivity fluorescence detector and the Nexera Ultra High Performance LC system, in which the aflatoxinsB1, B2, G1 and G2 are analyzed using direct high-sensitivity fluorescence detection without conducting TFA derivatization.
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39% High Speed, High Resolution Analysis (Part 41) Performance Evaluation of Carryover Using Reserpine in Human Plasma by the Nexera UHPLC System
Here, we introduce the results of the carryover evaluation of the Nexera SIL-30AC autosampler using the Nexera UHPLC (Ultra High Performance Liquid Chromatography) System and LC/MS/MS.
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39% Ultra-Fast, High-Sensitivity Analysis of Degradation Products in Valsartan
The need for reliable separation and quantification of minute quantities of pharmaceutical impurities suspected of genetic toxicity continues to grow. Using the SPD-M30A high-sensitivity photodiode array detector permits high-sensitivity detection of extremely trace amounts of components. Introduced here is an example of ultra-fast, high-sensitivity simultaneous analysis of valsartan degradation products.
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39% High-Speed and High-Sensitivity Analysis of Cefazolin Using SPD-M30A
Impurities present in drugs that are suspected of genotoxicity must be subjected to separation and quantitation at trace levels lower than typically required. With the SPD-M30A high-sensitivity photodiode array detector, even extremely trace level components can be detected and quantitated. Here we introduce an example of ultra-high-speed, high-sensitivity analysis of the degradation products of cefazolin.
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37% Impurity Analysis by High-sensitivity UV Detector
Analysis of impurities can be a challenging task especially whenthe main ingredient needs to be assayed at the same time, which requires a wider dynamic range and lower baseline disturbances. With an extra wide dynamic range, temperature-controlled flow cell and a stray-light correction function, both SPD-20A UV and SPD-M20A Photo Diode Array detectors offer a high level of stability, sensitivity andlinearity for a confident analysis. The 100 Hz sampling of the SPD-20A/M20A ensures peak information can be captured without loss of resolution and exploits the full potential of ultra-fast LC columns.
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37% Long-term Automated HPLC Analysis of Microdialysis Samples from Multiple Freely Moving Animals
Online microdialysis integrated with real-time HPLC analysis is a powerful tool for providing information on the circadian pacemaker, especially in combination with molecular and pharmacological tools. Other applications of this system are certainly possible for the long-term in-vivo analysis of various biological substances in freely moving animals. To access the full issue of LC World talk, please click here.
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37% Impurities Analysis in Pharmaceuticals: FDA Regulations on Genotoxic Impurities in Pharmaceuticals
The FDA draft guidance was issued to cover the verification of the genotoxicity and carcinogenicity of ultra-trace impurities in new pharmaceuticals, investigational new drugs, and generic pharmaceuticals by structural analysis of ultra-trace impurities. This demands structural analysis of ultra-trace impurities in a 200 mg daily drug dose at approximately 150 times lower levels than under the ICH guidelines. LCMS-IT-TOF analysis, which provides precise mass measurement and MSn spectra, is an effective method for the structural analysis of ultra-trace impurities. To access the full issue of LC World talk, please click here.
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37% Dual Wavelength Detection of a Peptide
When it comes to demanding HPLC applications, you need a reliable system providing the ultimate in flexibility and performance. The Shimadzu LC-10A Series is such a system. While every analysis is not as difficult to perform as trifluoroacetic acid (TFA)/acetonitrile peptide separations, wouldn't it be nice to have equipment which is equal to the task, reliable enough for routine applications and also a good value? Of course it would! That's why you need to look no further than Shimadzu's LC-10A.
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37% Improved R&D Efficiency Through Speedier Method Development (3)
Method scouting is the search for analytical conditions (column, mobile phase, and gradient settings) that optimize an LC separation. When used with a UHPLC (ultra high performance LC) system, the time to produce a robust, validated method is greatly shortened, which leads to an increased interest in and application of UHPLC systems for method development. In this report, we introduce an example of such scouting using the Nexera Method Scouting System together with its specialized Method Scouting Solution software to conduct a search for simultaneous analytical conditions to be used in analysis of 13 cephem antibiotic substances.
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37% Determination of Vitamin E in Hand Lotion
Fat soluble Vitamin E (alpha-tocopherol) is an oil found naturally in plants including wheat germ, rice and cotton seeds. In humans, Vitamin e is a potent antioxidant and is also involved in the metabolism of the trace element selenium.
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37% Determination of Tetrabutylammonium Ion in an Antibiotic Sample
Quaternary ammonium salts such as tetrabutylammonium bromide (TBA+Br-) are often encountered in organic chemistry. The reaction of a quaternary ammonium halide with silver oxide yields a quaternary ammonium hydroxide and a silver halide
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37% Automated Calibration Standard Preparation with the SIL-6B/9A
For HPLC analysis, it is often necessary to generate a calibration curve by injecting standards of several known concentrations and computing response factors based upon the linear regression of a plot peak area (or height) vs concentration.
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37% Applications of Prominence RF-20AXS Fluorescence Detector - Analysis of Saccharides Using Post-Column Derivatization System
Here we present some examples of analyzing saccharides using the reducing sugar analysis system (anion exchange mode) with the Prominence RF- 20AXS.
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37% Ion Analysis in Drugs - Determination of Counter-ions and Impurity Ions by Ion Chromatography
Here we present examples of analysis of counter-ions and impurity ions at micro levels in drugs using ion chromatography.
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37% Analysis of Dietary Supplements
Analytical Instrumentation & Software for the Evaluation, Identification and QA/QC of Dietary Supplements
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37% SIL-10AXL Autosampler Recommended Spare Parts List
The following is a list of recommended spare parts for the SIL-10AXL Autosampler. This list encompasses those consumable items which are most frequently replaced.
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37% SIL-10Ai Autosampler Recommended Spare Parts List
The following is a list of recommended spare parts for the SIL-10Ai Autosampler. This list encompasses those consumable items which are most frequently replaced.
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37% GPC Analysis of Wetting Agent
Gel permeation chromatography (GPC), is a method widely used in surfactant analysis because it separates the components based on molecular weight. Two GPC applications are presented: a metallic soap analysis and a non-ionic surfactant agent mixture analysis.
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37% Engineering Plastics Components
Engineering plastics include polyamide, polacetal, denatured polyphenylene oxide, polysulfonyl, polycarbonate, and polyester. The recent development of HFIP (hexafluoroisopropanol) has enabled the analysis of this group of engineering plastic compounds at room temperature.
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37% Analysis of Nucleotides Using ODS Column
Analysis of nucelotides is frequently performed using an ODS column in reverse phase distribution mode. Here, application examples demonstrate the analysis of ATP in rat heart tissue and the analysis of nucleotides in tuna meat.
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37% The HPLC Analysis of Black Cohosh Using an INA Method
The Institute for Nutraceutical Advancement (INA) is a consortium of research and commercial laboratories whose mission is the development of standardized methods for the analysis of natural products. A myriad of nutraceutical products is available to health-conscious consumers.
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37% ELSD and UV - Complementary Detectors
Reversed phase HPLC with UV detection at 210nm has been used to determine constituents in commercial Stevia products, but the methodology is limited in its ability to separate all components of interest, especially when higher order oligosaccharides are present.
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37% High Sensitivity Analysis of a Minor Impurity in Pharmaceuticals Using Co-Sense for Impurities System
Here, we introduce an example in which the Co-Sense for Impurities System is applied to a drug impurity analysis, making it possible to conduct measurement quickly and easily through automation of the sample preparation.
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37% Improvement of Dye-Sensitized Solar Cell Efficiency ThroughHigh Purification of Dye
Here in Application News L427, we introduce an example of improved solar cell performance achieved through high purification of a commercially available N3 dye using HPLC.
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37% Post-Column pH Buffered Electric Conductivity Detection of Organic Acids with Ultra High-Performance Liquid Chromatography
Here, we introduce an example of ultra fast simultaneous analysis of organic acids in which this detection technique was applied to organic acid separation by UHPLC.
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35% Improved R&D Efficiency Through Speedier Method Development (1)
Various approaches are available for improving the efficiency of analytical method development. The introduction of high-speed analysis into the method development process for LC and LC/MS is one approach that can directly enhance development efficiency by simply speeding up chromatographic evaluation.
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35% Application of Metabolomics Techniques using LC/MS and GC/MS Profiling Analysis of Green Tea Leaves
This Application Note presents the profiling of green tea leaves as an example of one application of the metabolome analysis technique using LC/MS and GC/MS. A search of the substances believed to play roles in product quality was conducted, and a quality prediction model was constructed based on the results.
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35% Guaranteed Scale-Up From Analytical to Preparative with Shimadzu Matched Column Sets
Development of an HPLC can be a time consuming process. Development of a preparative HPLC method can be expensive as well as time consuming, when the cost of the solvents being pumped at preparative flow rates is taken into consideration. Shimadzu provides a solution to this problem by offering matched column sets with the scale-up from analytical to preparative guaranteed.
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35% Fraction Collection - B Carotene in Carrot Extract
b-carotene is contained in carrots, pumpkins, tomatoes, and other produce. It is an essential vitamin A precursor. An HPLC recycling valve separation technique was developed for the analysis of b-carotene from carrot extract. The effect of the recycling method was then confirmed using a photodiode array detector.
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35% Quantitative Analysis of Carbohydrates by LC/MS
Here we introduce examples of quantitative analysis of carbohydrates in foods using post-column solvent addition.
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35% Analysis of Anion in Environmental Water
Shows examples of Shimadzu HPLC Analysis of environmental water by the LC-6A inorganic ion analysis system
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35% Confirmation of Aureomycin and Urea in Livestock Feed Mix by LC/MS
A livestock feed was analyzed to confirm the presence of urea and aureomycin, 7-cholrotetracycline
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35% Fraction Collection Options with Discovery VP
The Discovery VP preparative system is designed for purification of compounds generated by reaction processes such as combinatorial chemistry and multi-step organic synthesis, or for isolation of analytes in naturally occurring substances.
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35% High-Speed Analysis of Chlorogenic Acid
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35% LC-MS Analysis of Carbohydrates Using Post ColumnAddition of Solvent for Improved Ionization Efficiency
Typically, in the HPLC analysis of saccharides, two different modes of chromatography can be used: aqueous normal phase, or ligand exchange mode. Due to the difficulty in completely separating all of the saccharides, these are used in a complementary manner. Since salts that are not easily volatilized cannot be used in LC-MS analysis, the suitability of the HPLC conditions for LC-MS analysis depends on the choice of the mobile phase. Because acetonitrile - water (or ammonium acetate in water) is generally used as the mobile phase in the aqueous normal phase mode, and only water is used as the mobile phase in the ligand exchange mode, these can be considered suitable mobile phases for LC-MS analysis. While these conditions are very suitable for the HPLC separation of mixed carbohydrates, there can be difficulty in observing an ion which up to then has been easily confirmed, or there may be a loss of sensitivity due to the separation column or the HPLC conditions.
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35% Drugs of Abuse: Benzodiazepines and Barbiturates
A rapid, accurate, and reliable LC-MS-MS method was developed for the determination of 21 controlled substances in urine.
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35% Designer Cannabinoids
A rapid LC-MS-MS method for determination of designer cannabinoids in smokeable herbs was developed.
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35% Mycotoxins
An extremely fast LC-MS-MS method for the determination of mycotoxins and other untargeted chemical threats was developed.
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34% Melamine Analysis Using Triple Quad LCMS-8030
This fast and reliable LC/MS/MS method for melamine and cyanuric acid is over five times faster than conventional methods for these compounds, with a run time under 60 seconds. The simple sample preparation involves only dilution and a one-step solvent extraction and can be automated for high-throughput sample analysis. The sensitivity is more than adequate to meet regulatory requirements while the speed and reliability enable rapid analysis of large numbers of samples. To access the full issue of LC World talk, please click here.
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33% High Speed, High Resolution Analysis (Part 41) Carryover Evaluation of Glibenclamide in Human Plasma by Nexera HPLC
LC/MS/MS high-speed, high-resolution analysis methods with columns packed with particles of 2 µm or less are often used for analysis of drugs in human blood plasma. The small particle columns produce dispersion that is even less than that obtained with conventional columns (5 µm particle size), so peaks are taller, thereby enabling quantitation at even higher sensitivity. While this high-sensitivity analysis has become possible, the effect of autosampler carryover on high-sensitivity LC/MS/MS analysis has become a problem. In Application News No. L425, we evaluated the performance of the Nexera SIL-30AC autosampler in the analysis of reserpine in human plasma, and reported its low carryover performance. In addition to the high potential of the SIL-30AC, we believe that rinsing with its powerful and sophisticated multi-solvent configuration was very effective.Here, we report on the effectiveness of the rinse method reported in Application News No. L425, and demonstrate its effectiveness as a rinse method applicable to measurement of a wide range of drugs in plasma.
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33% Quantitation by HPLC and Identification by LC/MS for Total Aflatoxins in Food
Aᜀatoxins are mycotoxins that are extremely carcinogenic and acutely toxic. In Japan, foods in which the total aflatoxins (sum of B1, B2, G1 and G2 aflatoxins) are detected at levels exceeding 10 µg/kg are in violation of Article 6, Item 2 of the Food Sanitation Act1).Previously, in Application News articles L422, L428 and L430, we introduced examples of simultaneous analysis of these 4 substances (B1, B2, G1 and G2) using HPLC and UHPLC. In accordance with the test method2) specified in the notification of August 16, 2011, after pretreatment using an immunoaffinity column, we conducted (1) quantitation using an HPLC system compliant with the test method, (2) identification using a LC/MS system compliant with the test method, and (3) rapid quantitation and identification using the Nexera UHPLC. Here, we present these analyses in addition to the results of the validation.
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33% LC/MS/MS Analysis of Vitamin D by the Co-Sense for Impurities System
The Co-Sense for Impurities system, as previously described in Application News No. L424, is a complete HPLC configuration that automates the sample pretreatment process in trace level analysis. The earlier article presented an example of high sensitivity detection using analyte extraction and concentration. This system offers analyte concentration and removal of
impurities, and therefore comprises 3 flow lines: one for primary separation, another for concentration, and the third for secondary separation. Since a different mobile Detector B
phase and column can be selected for each flow line, a combination of conditions can be used, including those that are conducive for separation at the primary separation stage and that are appropriate for detection for the secondary separation stage. Here we introduce
an example in which Vitamin D3 capsules were analyzed
by LC/MS/MS using such a method.
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33% Ultra-High Speed Analysis of Ibuprofen by Nexera in Accordance with USP 621
High throughput analys is has been advancing dramatically in recent years with the increasing necessity to improve productivity and operational efficiency. HPLC has also been in the spotlight thanks to significant advances in ultra-high-speed analysis technology, in particular ultra high performance LC and micro-particle column packing material. The recently revised General Chapter 621 of the United States Pharmacopoeia (USP 621) now permits a degree of adjustment of HPLC and GC parameters, specifically aimed at satisfying the requirements of system suitability. Here, using the Nexera ultra high performance liquid chromatograph and the Shim-pack VP-ODS conventional column, in addition to the Kinetex XB-C18 Series Core-Shell fast analysis column, we introduce examples of high-speed analysis of ibuprofen-related substances in conformance with USP 621.
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31% Identification of Proteins by LC-MALDI System (2) - Analysis of Carbonylated Proteins
Using the AXIMA MALDI-TOF-MS system for end-point analysis of two-dimensional electrophoresis, we analyzed carbonylated proteins in the CA1 area of a monkey hippocampus affected by ischemia-reperfusion.It is known that reactive oxygen species, or oxygen radicals (ROS), negatively impact a great many diseases, including cancer, cardiac disease and cerebral stroke, which are the leading causes of death among the Japanese, in addition to such lifestyle diseases as diabetes and arteriosclerosis.
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31% High Speed with High Resolution Analysis -Analysis of humulones and lupulones in Hops
Hops are one of the basic ingredients in beer and contain -acids (humulones) and β-acids (lupulones). During the brewing process the Α-acids are converted through isomerization into iso-Α-acids (isohumulons), which are largely responsible for the bitterness of beer. β-acids, on the other hand, do not produce much bitterness during the boiling stage of beer production, but they are said to play a role in "balancing" the bitter flavor during fermentation and storage. HPLC is generally used for the analysis of Α-acids and Β-acids, but this analysis typically takes as long as 30 minutes. Here we introduce an example of high-speed analysis of Α-acids and Β-acids in hops using the Prominence UFLCXR ultra fast, high resolution LC system with a high-speed, high-resolution column.
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31% High Speed with High Resolution Analysis - High Efficiency Analysis of Ferulic Acid in Berry Juice Using Automated Column Switching System
Here, we introduce an example of analysis of ferulic acid in berry juice using a system constructed specifically for efficient handling of this application. Shimadzu’s Prominence UFLC ultra fast LC system forms the base of the system, which also includes a column switching system comprising the first dimension separation column, a trap column, and the second dimension separation column.
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31% Applications of the Prominence RF-20AXS Fluorescence Detector - High-Speed Analysis of Tocopherols and Polycyclic Aromatic Hydrocarbons
The Prominence RF-20AXS fluorescence detector not only achieves higher sensitivity than ever before possible (water Raman SN ratio greater than 2000), it also includes as a standard feature a temperature controlled cell with a cooling function to provide highly reliable detection irrespective of ambient temperature fluctuations. Moreover, its excellent performance as a fluorescence detector for ultra fast LC is demonstrated with its high-speed sampling and fast response. Here we introduce examples of high-speed analysis of tocopherols and polycyclic aromatic hydrocarbons using the Prominence RF-20AXS.
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31% High Speed, High Resolution Analysis (Part 33)
Ginger is not only used as a spice, but has also been used in traditional Chinese herbal medicine since ancient times. Investigation into the efficacy of ginger has been attracting a lot of attention in recent years, and the use of ginger in health foods has seen great increases. Here we introduce an example of the analysis of 6-gingerol and 6-shogaol, constituents of ginger, using the Prominence UFLC ultra fast LC system with the SPD-M20A photodiode array detector.
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31% The Application of UHPLC to Reduce Sample Analyses Times
The use of UHPLC technology has continued to increase in LC applications as a way to reduce sample analysis turnaround times. Maintaining or increasing resolution of faster separations has been made possible with the use of small particle column technology. UHPLC often provides run times of less than one minute with peak widths of less than 200 milliseconds that have created challenges for system performance and detection methods. A variety of applications in pharmaceutical and environmental areas with small particle columns will be presented that will demonstrate the throughput and performance that can be obtained with current UHPLC technology.
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31% The Ultimate in Speed and Resolution
The Nexera addresses all analysis requirements : conventional / ultra-high speed / ultra-high resolution / high temperature, with no compromise in data integrity. The wide pressure range increased up to 130 MPa allows Nexera to maximize performance of sub-2-micron particles column. The combination of maximized pressure range and high resolution columns such as sub-2 micro columns realizes genuine ultra high speed and ultra high resolution analysis. The following are the examples of analytical data obtained using the NexeraUHPLC. The data demonstrates the ultra-high speed and ultra-high resolution that can be obtained with this system.
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31% The UHPLC with the Finest Reproducibility under Ultra-High Speed
Drawing upon innovations developed by Shimadzu across all industries and applications Nexera achieves superb reproducibility of retention time, separation and the peak area through accurate control of the injection volume, temperature, precise solvent delivery and mixing of solvents. The micro plunger-driven precision solvent delivery control can keep up with the demanding flow rate changes required during the rapid concentration shifts in fast gradient programming. Nexera also offers a ultra-low volume mixer ( 20 μL) based on micro reactor technology. The data demonstrates ultra-fast gradient analysis of alkylphenones.
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31% Achieving Near Zero Carryover
Nexera autosampler has been designed to provide our customers with the next generation tools for combating sample carryover on an entirely new level. It is now possible to perform a thorough rinse of the sample path and the needle surface by multiple different solvents eliminating the traces even of the stickiest of compounds. This is in addition to the flow-through needle design with minimized contact area which by itself significantly reduces carryover and insures highly reproducible analysis of trace compounds.
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31% Use of Methanol Solvent for Separation
In liquid chromatography acetonitrile is often a mobile phase ingredient of choice because of its elution strength in reverse phase analysis, low viscosity, low absorption in a wide range of the UV spectrum, and relatively low cost. However, in order to improve peak selectivity, researchers sometimes need to look for alternative solvents. Due to a wide pressure range of up to 130 MPa Nexera affords unlimited flexibility in choosing the ideal composition of the mobile phase even utilizing such viscous solvents as methanol-water that significantly increase the back pressure.
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31% Ultra-High-Resolution Analysis of Aromatic Carboxylic Acids
Plants and processed foods contain a variety of hydroxycinnamic acid derivatives and hydroxybenzoic acid derivatives. A high-resolution column is required for their analysis to achieve mutual resolution and resolution from the impurity components in the sample. Methods using a long, sub-2 µm column are available to achieve high resolution, but they demand a UHPLC system with high pressure tolerance. The Nexera 130 MPa pressure tolerance offers adequate capacity to meet these requirements. This Application Data Sheet introduces the ultra-high-resolution analysis of aromatic carboxylic acids using Shimadzu Nexera with a Shim-pack XR-ODS III column.
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31% Ultra-High-Speed Analysis of Quinolone Antibacterial Agents
Quinolone antibacterial agents are widely used in livestock, poultry, andfarmed fish to prevent and treat infectious diseases. Many types are available. Such simultaneous analysis of multiple components generally requires a long analysis time. However, the combination of a Shim-pack XR Series fast-analysis column and Nexera can significantly reduce the analysistime required. This Application Data Sheet introduces the ultra-high-speed simultaneous analysis of quinolone antibacterial agents using Nexera with an RF-20Axs high-sensitivity fluorescence detector.
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31% Ultra-High-Speed Analysis of Polyphenolsin Wine
Red wine contains many types of polyphenols. One of them, resveratrol, is attracting a great deal of attention as an antioxidant that may have the ability to extend life. A high-resolution column is used to resolve the impurity components for the analysis of resveratrol in red wine. A fluorescence detector is effective from both thesensitivity and selectivity viewpoint. This Application Data Sheet introduces the ultra-high-speed analysis of resveratrolin red wine using Nexera with an RF-20Axs high-sensitivity fluorescence detector.
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31% Ultra-High-Resolution Analysis of Polycyclic Aromatic Hydrocarbons
As the analysis of polycyclic aromatic hydrocarbons in the environment demands the highly efficient resolution of multiple components, long sub-2 µm columns or multiple columns are often used. Such cases demand a UHPLC system with a high system pressure tolerance. The 130 MPa pressure tolerance of the Shimadzu Nexera can safely and flexibly accommodate such demands. This Application Data Sheet introduces the ultra-high-resolution simultaneous analysis of polycyclic aromatic hydrocarbons using Nexera with two Phenomenex Kinetex columns.
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31% Ultrafast Analysis of Synthetic Colorants
Various types of synthetic colorants are used as food additives, and gradient elution is generally used to analyze them. Nexera enables stable, ultrafast gradient elution through accurate solution delivery and the use of a high-efficiency gradient mixer. This document introduces an example of ultrafast analysis performed on 12 tar synthetic colorants using Nexera and a Phenomenex Kinetex C18 column (particle size 2.6 µm, a core-shell column where a 0.35 µm porous membrane is combined with a 1.9 µm solid core).
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31% Ultrafast Analysis of Water-Soluble Vitamins
Water‐soluble vitamins such as thiamine (vitamin B1) and riboflavin (vitamin B2) are generally analyzed by reverse‐phase ion‐pair chromatography. This document introduces an example of ultrafastanalysis performed on water‐soluble vitamins by gradient elution using Nexera and a Phenomenex Kinetex C18 column (particle size 2.6µm, a core‐shell column where a 0.35µm porous membrane is combined with a 1.9µm solid core).
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31% Ultrafast, Ultrahigh-Sensitivity Analysis of Aflatoxins
Aflatoxins are mycotoxins with a carcinogenic property and acute toxicity. They are measured by fluorescence detection HPLC and other instruments in order to prevent contaminationof foods. Among four components (B1, B2, G1, G2) quantitated as the total aflatoxin, sensitivity for B1and G1is generally enhanced by a derivatization process using trifluoroacetic acid. Using the RF‐20Axs High‐Sensitivity Fluorescence Detector enables these components to be directly detected at a high sensitivity without any derivatization process. This document introduces an example of ultrafast, high‐sensitivity analysis performed on aflatoxins using Nexera and the RF‐20Axs
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31% High Speed, High Resolution Analysis (Part 33)Analysis of Gingerol and Shogaol in Ginger
Ginger is not only used as a spice, but has also been used in traditional Chinese herbal medicine since ancient times. Investigation into the efficacy of ginger has been attracting a lot of attention in recent years, and the use of ginger in health foods has seen great increases. Here we introduce an example of the analysis of 6-gingerol and 6-shogaol, constituents of ginger, using the Prominence UFLC ultra fast LC system with the SPD-M20A photodiode array detector.
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31% High Speed, High Resolution Analysis (Part 35) Analysis of Resveratrol in Wine by the Nexera UHPLC System
Resveratrol, a type of polyphenol, is a phytoalexin that is synthesized by plants when they are exposed to stress due to disease and pests. In addition to providing antioxidative effects, resveratrol is reported to offer other health benefits including support for increased longevity. For these reasons, this substance is actively being researched, and recently, resveratrol is being added to food products and to cosmetics. Here we introduce an example of ultra fast analysis of resveratrol in red wine using the Nexera ultra high performance liquid chromatograph and the Prominence RF-20Axs high-sensitivity fluorescence detector.
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31% Direct LC/MS Analysis of Basic Drugs in Plasma by On-line pretreatment with Weak Cation-Exchange Pretreatment Column
A new pretreatment column, Shim-pack MAYI-WCX (prototype), which has weak cation-exchange groups as stationary phase, has been developed for trapping of basic compounds in consideration of LCMS use. The retention ability and pretreatment effect of the Shim-pack MAYI-WCX was evaluated for desipramine, dibucaine, lidocaine, verapamil and amitriptyline by using a column-switching system (Co-Sense for BA system, Shimadzu).
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31% Ultra Fast Method Scouting − Maximizing Efficiency for Method Development
The traditional process of investigating analytical conditions during method development is often complicated and time-consuming, and only when it becomes possible for anyone, regardless of skill level, to carry out this task easily, quickly, and ideally as an automated procedure, will greater speed and efficiency raise the overall level of analytical operations. This Report introduces the Nexera Method Scouting system, a UHPLC (ultra high performance LC) system for identifying and resolving the limiting factors associated with traditional method development techniques.
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31% Ultra Fast Method Scouting − Maximizing Efficiency for Method Development
The traditional process of investigating analytical conditions during method development is often complicated and time-consuming, and only when it becomes possible for anyone, regardless of skill level, to carry out this task easily, quickly, and ideally as an automated procedure, will greater speed and efficiency raise the overall level of analytical operations. This Report introduces the Nexera Method Scouting system, a UHPLC (ultra high performance LC) system for identifying and resolving the limiting factors associated with traditional method development techniques.
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31% Ultra-Fast, High-Sensitivity Analysis of Artificial Colorings Using the SPD-M30A Photodiode Array Detector
The newly-designed capillary SR-Cell (Sensitivity and Resolution Cell) has been adopted for the SPD-M30A high-sensitivity photodiode array detector in the Nexera SR Ultra High Performance Liquid Chromatograph. By optimizing the cell's optical path length and diameter, both low noise levels and high sensitivity have been achieved. This article introduces the high-speed, high-sensitivity simultaneous analysis of artificial colorings using the Nexera SR UHPLC.
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31% High-Speed, High-Resolution Analysis of Water-Soluble Vitamins Using SPD-M30A
The Nexera SR is a high-end model within the Nexera X2 series of ultra high performance liquid chromatographs. It features the SPD-M30A high-sensitivity photodiode array detector which incorporates the newly designed capillary SR-Cell (Sensitivity and Resolution Cell). Optimization of the optical path length and diameter results in both high sensitivity and low noise. Introduced here is an example of high-speed, high-sensitivity simultaneous analysis of water-soluble vitamins using the Nexera SR ultra high performance liquid chromatograph.
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31% Ultra High-Sensitivity Analysis of Degradation products in Valsartan Using the SPD-M30A Photodiode Array Detector with High-Sensitivity Cell
The need for reliable separation and quantification of minute quantities of pharmaceutical impurities suspected of genetic toxicity continues to grow. Using the SPD-M30A high-sensitivity photodiode array detector permits high-sensitivity detection of extremely trace amounts of components. Introduced here is an example of ultra-fast, high-sensitivity simultaneous analysis of valsartan degradation products.
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31% Ultra-Fast and High-Sensitivity Analysis of Artificial Colorings Using the SPD-M30A Photodiode Array Detector
The newly-designed capillary SR-Cell (Sensitivity and Resolution Cell) has been adopted for the SPD-M30A high-sensitivity photodiode array detector in the Nexera SR Ultra High Performance Liquid Chromatograph. By optimizing the cell's optical path length and diameter, both low noise levels and high sensitivity have been achieved. This article introduces the high-speed, high-sensitivity simultaneous analysis of artificial colorings using the Nexera SR UHPLC and a high-sensitivity cell (option).
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31% Ultra High-Sensitivity Analysis of Water-Soluble Vitamins Using SPD-M30A with High-Sensitivity Cell
The Nexera SR is a high-end model within the Nexera X2 series of ultra high performance liquid chromatographs. It features the SPD-M30A high-sensitivity photodiode array detector which incorporates the newly designed capillary SR-Cell (Sensitivity and Resolution Cell). Optimization of the optical path length and diameter results in both high sensitivity and low noise. Introduced here is an example of high-speed, high-sensitivity simultaneous analysis of water-soluble vitamins using the Nexera SR ultra high performance liquid chromatograph with high-sensitivity cell (option).
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31% Analysis of Anionic Surfactants Using the RF-20A Fluorescence Detector
In the analysis of anionic surfactants, of which standard values are specified by the water quality standards in the Japanese Water Supply Act, 250 mL of test water is subjected to 250-fold concentration using a solid phase extraction cartridge. The surfactants are then detected with a fluorescence detector after separation utilizing a reversed phase separation column. In this case, a C18 column, which uses the octadecyl silyl group (C18) as the solid phase, was used. Anionic surfactants C10 to C14, which are subject to analysis, include branched chains. Thus, approximately 20 isomers are separated.
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31% Analysis of Dicyandiamide in Fertilizers
The dicyandiamide contained in calcium cyanamide is used as a type of nitrification suppressant to inhibit ammonia from changing into nitric acid. In accordance with the partial revision to the matters specifying official standards for general fertilizers based on the Fertilizers Regulation Act (The Ministry of Agriculture, Forestry and Fisheries of Japan; Notification No. 1985; dated August 8, 2012), the content of dicyandiamide-nitrogen in calcium cyanamide is to be 20.0 % or less of the total nitrogen content. This article introduces an example of the analysis of dicyandiamide in fertilizer, with reference to the fertilizer test method (2012) under the supervision of the Japan‘s Food and Agricultural Materials Inspection Center (FAMIC).
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31% Simultaneous Analysis of Formic Acid and Formaldehyde
Artificial photosynthesis uses photocatalytic reactions for water splitting and carbon dioxide reduction to create hydrogen, oxygen, carbon monoxide, and formic acid. Of these reaction products, hydrogen, oxygen, and carbon monoxide escape from the sample solution as gases. However, formic acid remains in the sample together with added components, such as electron donors, and byproducts, such as formaldehyde. To measure the amount of formic acid created, it is necessary to separate it from these components. This Data Sheet highlights the pH-buffered electroconductivity method offered by the Shimadzu Organic Acid Analysis System to analyze formic acid, together with a refractive index detector for the simultaneous analysis of formaldehyde.
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31% High-Speed Analysis of Amino Acids and Histamine in Fish Sauce via Automated OPA Pre-column Derivatization
Processed foods and fish containing a significant amount of histamine have resulted in several cases of allergic food poisoning. In Japan, no standard values have been set for histamine concentrations in foods. However, the FDA has specified levels of 50 mg/kg max. for foods in general, the EU has specified 100 mg/kg max. for marine products, and the Codex Alimentarius standard specifies 400 mg/kg max. for fish sauce.
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31% Effect of Column Temperature on Organic Acid Separation
Shimadzu's organic acid analysis system utilizes ion exclusion chromatography to first separate the target components, then introduce a pH buffer solution by the post-column method to adjust the pH to near neutral, and detect the organic acids using a conductivity detector to permit quantitation of the various organic acids detected. To increase the peak capacity for separations with large numbers of components, this system is often equipped with two columns connected in series. Although the temperatures of these columns are usually set to the same value, setting their temperatures to different values permits adjustment of the separation conditions to improve the resolution of closely eluting organic acids. Here we report the results of analysis of a mixture of organic acids using a dual-column, dual-temperature method.
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31% Effective Method Development Using Online Blending of Organic Solvents for Mobile Phase Preparation
When investigating the analytical conditions during HPLC method development, selection of the mobile phase greatly influences the separation. In reversedphase analysis, for example, when the retention and peak shape of an analyte vary greatly depending on the organic solvent used, the separation can often be improved by blending together various organic solvents. However, from the analyst's perspective, every organic solvent mixture prepared must be tested in the actual separation a process that requires a great deal of time and effort. Here, we introduce a technique in which multiple organic solvents are blended online to prepare a mobile phase that is suitable for analysis.
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31% Effective Method Development Using Online Blending of Buffer Solutions for Mobile Phase Preparation
When investigating analytical conditions during HPLC method development, selection of the mobile phase greatly influences the separation. In particular, when the analyte is an ionic compound, it is especially important that the mobile phase contains a buffer solution of appropriate pH because the analyte retention varies greatly depending on its ionic dissociation equilibrium. From an analyst's perspective, however, the preparation of several mobile phases at various pH values for each analysis in the investigation of the analytical conditions represents a tedious and time-consuming effort. Here, we introduce a technique in which multiple solutions are blended online to control and adjust the pH of mobile phases for use in analysis.
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31% Effective Method Development Using Online Blending of Ion-Pair Reagents for Mobile Phase Preparation
When investigating analytical conditions during HPLC method development, selection of the mobile phase is extremely important due to its effect on analyte separation. In ion-pair reversed-phase mode analysis, the column retention strength changes depending on the concentration of the ion-pair reagent that is added. Therefore, the ion-pair reagent concentration is very important when considering the analytical conditions. However, from the analyst's perspective, the preparation of several mobile phases with different concentrations of ion-pair reagent for each analysis in the investigation of the analytical conditions represents a tedious and time-consuming effort. Here, we introduce a technique in which multiple variants of mobile phase containing different concentrations of ion-pair reagent are prepared online for use in analysis.
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30% Identification of Proteins by LC-MALDI System (1) - Analysis of Samples Obtained from Frozen Tissue Sections
We conducted proteome analysis using a two-dimensional gel electrophoresis system and frozen tissue sections obtained by laser microdissection (LMD).Two-dimensional gel electrophoresis is a technique that is recognized for its excellent reproducibility and its ability to quantitate. Therefore, it is still the most widespread analytical method in proteomics.
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30% Application of Evaporative Light Scattering Detector - Analysis of Terpenoids in Ginkgo Biloba
Ginkgo biloba extract contains flavonoids and terpenoids that have been reported to be effective for improving poor blood circulation in the brain as well as poor peripheral blood vessel circulation. This ginkgo biloba extract is used as a health dietary supplement in Japan and the United States. Here we present an example of analysis of terpenoids in ginkgo biloba extract using the ELSD-LT II evaporative light scattering detector.
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30% Analysis of Ethyl α-D-Glucoside in Sake
Japanese sake typically contains ethyl α-D-glucoside (α-EG), a substance believed to promote beautiful skin, and it is now receiving attention for its effectiveness in improving dry skin conditions even when taken in a drink. Here we present an example of analysis of ethyl α-Dglucoside and other saccharides in sake using hydrophilic interaction liquid chromatography (HILIC).
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30% High Speed, High Resolution Analysis (Part 30) Analysis of Iso-α-Acids and α-Acids in Beer
Here we introduce an example of analysis of iso-α-acids and α-acids using the ultra-high speed, high resolution Prominence UFLCXR system with a high-speed, highresolution column.
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30% Analysis of Various Vitamins in Multivitamin Tablets
This application news displays the analysis of various vitamins in multivatamin tablets according to the USP proposed techniques for the analysis of dietary supplements.The United States Pharmacopeia (USP) is an official authority that sets standards for all prescription and over–the–counter drugs, dietary supplements and food ingredients that are intended for sale in the United States.
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30% High Speed, High Resolution Analysis (Part 32) Analysis of Phthalates Using Shim-pack XR Series Columns
Phthalate is the generic term for the esters combined phthalic acid and various alcohols that are widely used as a plasticizer for various synthetic resin. Here we present the retention behavior and examples of high-speed analysis of phthalates using the ultrahigh speed Prominence UFLC system and various high-speed, high-resolution Shim-pack XR series columns.
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30% Ultra High Performance Liquid Chromatography / Mass Spectrometry Using Open Solution Software
The LCMS-2020, with its high scanning speed (up to 15,000 u/sec) and high-speed polarity switching (15 msec polarity switching), demonstrates the performance that satisfies the demands of UHPLC. The powerful user interface of Open Solution makes data review and verification easy, supporting the chemist who wishes to make rapid yet accurate decisions related to his ongoing research.
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30% Applications of the Prominence RF-20Axs Fluorescence Detector (Part 5)
Voglibose is a diabetes drug which inhibits the activity of α-glucosidase. HPLC post-column derivatization is specified as the test method for voglibose in the Japanese Pharmacopeia, Fifteenth Edition (voglibose purity test, quantitation of voglibose in pharmaceutical tablets). Here we introduce an example of voglibose tablet testing as specified in the Japanese Pharmacopeia, Fifteenth Edition, using the Prominence post-column derivatization system with the Prominence RF-20Axs fluorescence detector.
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30% Analysis of Flavonoids in Ginkgo Biloba Extract
Ginkgo biloba leaves are said to contain as many as 20 types of flavonoids,and among these are quercetin, kaempferol and isorhamnetin, 3 types that are present in large quantities.Here we introduce an example of the analysis of these 3 favonoids present in ginkgo biloba leaves using the SPD-M20A photodiode array detector.
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30% High Sensitivity Analysis of Pirimicarb, Tetracycline, Oxytetracycline, o-Phenylphenol, and Diphenyl
Here we use the Prominence RF-20AXS fluorescence detector to introduce examples of high sensitivity analysis of pirimicarb, tetracycline, oxytetracycline, o-phenylphenol, and diphenyl as individual test method targets based on the Positive List System.
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30% Application of Evaporative Light Scattering Detector (Part 9)Analysis of Dimethicone (Dimethylpolysiloxane) in Hair Care Products
Dimethicone (dimethylpolysiloxane), a type of silicone oil, is widely used in hair care products such as conditioners and shampoos as an emollient that effectively utilizes the water repellent property of this substance.Here, we introduce examples of analysis of dimethicone in hair care products using the ELSD-LD II evaporative light scattering detector.
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30% Excellent Gradient Precision with the LPM-600 Low Pressure Quaternary Gradient Mixer
A key function of any gradient HPLC System is the ability to provide accurate and reproducible gradients. A lack of precision will result in fluctuating retention times and subsequent problems in quantitation. The Shimadzu LPM-600 Quaternary Gradient Mixer meets the requirements of precision gradients for analytical up to semi- preparative applications.
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30% Comparison of Semi-micro and Analytical HPLC
Development of an HPLC method can be very expensive and time consuming, especially when using the normal analytical columns and standard flowcells. Taking into account the amount of mobile phase used for these methods, the cost can increase tremendously. Shimadzu provides a solution to this problem by providing semi-micro flowcells for the SPD-10A(V), UV-Vis and RF-10A Fluorescence Detectors which can be used in conjunction with micro columns.
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30% Enhanced Fluorescence Sensitivity with Wavelength Programming
The Shimadzu RF-10A is a very sensitive, very flexible fluorescence detector that can be integrated with any HPLC System. Its various design features offer both high sensitivity and high selectivity. The RF-10A can perform excitation and emission wavelength scanning, and when linked via fiber optic to the SCL-10A System Controller, spectra can be viewed on the LCD display of the controller.
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30% HPLC Analysis of a Cold Tablet Using a 2.0 mm I.D. Column
HPLC analyses most commonly employ 4.6 mm ID columns and flow rates of 1.0 ml/min. However, the use of micro bore columns offer several advantages over the more common 4.6mm columns. The lower flow rates which are used mean that the consumption of solvent is markedly reduced.
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30% Updated Performance Comparison of Variable Wavelength and Photodiode Array Detectors
Shimadzu strives to achieve superior detector performance by designing high quality optical benches. To achieve this in both the UV and Vis range, dual lamp detector design is offered for both variable wavelength and PDA models. The purpose of this bulletin is to compare the relative performance of Shimadzu's UV-Vis and Photodiode Array Detectors.
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30% Herbal Medicine Analysis Application
The active ingredients of Asian herbal medicines vary with their harvest times and growing region. Their quality is determined by the amount of active ingredients they contain. These medicines may contain more than one active ingredient, which heighten their effectiveness by working synergistically. It is therefore often necessary to analyze for more than one medicine or active ingredient.
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30% Simultaneous UV Fluorescence Quantation of Neopterine, Uric Acid, Creatinine in Urine
Neopterine in urine makes up approximately 20-50% of the total pterine, and may be clinically related to blood and liver diseases. The neopterine concentration in urine requires creatinine correction; therefore, simultaneous determination of uric acid, creatinine, and neopterine is indispensable.
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30% Quantitation of Catechol Estroben in Plasma
Recently, the catechol estrogen in biological samples has received a great deal of attention due to its physiological actions. Radio-immuno Assay (RIA) is currently used as the analysis method. However, 2-hydroxyestrogen is extremely unstable and easily oxidized, and is therefore found only in small amounts. This results in poor sensitivity for analysis by the RIA method. The Shimadzu L-ECD-6A electrochemical detector, however, permits highly sensitive HPLC analysis for catechol estrogen and estrogen compounds.
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30% Assessing HPLC Peak Purity with Shimadzu Photodiode Detector for SPD-M10Avp
Traditionally, HPLC with a UV detector analysis generates chromatograms that present the analyte absorbance at a given wavelength as a function of the time. With the advent of the photo diode array detector, representation of the data in 3-dimensions is accomplished by adding the spectra if the analyte to the chromatogram
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30% SIL-HTc COMPETITIVE CARRYOVER PERFORMANCE ANALYZED BY LC-MS/MS: a Collaborative Study between Shimadzu Marketing Center and Covance Laboratories
Advances in the field of drug discovery have resulted in a tremendous production of potentially therapeutic compounds and, thus, another “bottleneck” downstream from synthesis to drug development.
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30% High Speed Analysis of Explosives
A number of high-speed columns that employ the use of sub 2 micron particles have been fairly recently introduced and can be used for high-speed analysis of explosive compounds. Here, a high-speed explosives method was developed on a larger particle size fused core column that will provide a more rugged assay. Isocratic and gradient conditions were developed for a mixture of EPA 8330 explosive standards.
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30% Fast Analysis of Vitamins in Dietary Supplements Using LCMS
Utilizing an ultra-fast LCMS with high-speed scan capability and high-speed data acquisition to achieve good linearity and repeatability, as well as accurate quantitative analysis of water-soluble vitamins in dietary supplements.
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30% A Practical Approach to Conserving Acetonitrile
Shimadzu offers reasonable and practical analytical tools and solutions to the global acetonitrile shortage. The use of small particle columns with smaller column dimensions has become an increasingly popular way to reduce analysis times, and also provides the additional benefit of decreasing mobile phase consumption.
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30% Application of Evaporative Light-Scattering Detector (Part 10) High Speed Analysis of Glucosylceramide
Here, we introduce an example of high-speed analysis of glucosylceramide in a dietary supplement using the ELSD-LTⅡ evaporative light-scattering detector connected to the Nexera UHPLC (Ultra High Performance Liquid Chromatography) System.
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30% High Speed, High Resolution Analysis (Part 37)Analysis of Clobazam and Cibenzoline in Serum by the Nexera UHPLC System
Here, we introduce an example of ultra-high-speed analysis of clobazam and cibenzoline in serum using the Nexera UHPLC (Ultra High Performance Liquid Chromatography) system and the Shim-pack XR-ODSⅢ high-speed separation column (particle size: 1.6 μm).
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30% High Speed, High Resolution Analysis (Part 38) Analysis of Artificial Colorings with the Nexera UHPLC System
In this issue, we present an example of ultra-high speed analysis using the Nexera UHPLC (Ultra High Performance Chromatography) System together with the Phenomenex Kinetex C18 high-speed, high-resolution analytical column, developed with the goal of achieving even greater analysis speeds.
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30% High Speed, High Resolution Analysis (Part 40) Analysis of Nucleobases, Nucleosides, and Nucleotides by the Nexera UHPLC System
Here, using the Nexera UHPLC (Ultra High Performance Liquid Chromatography) System, and the Shim-pack XR-ODSⅢ and Phenomenex Kinetex C18 high-speed, high-resolution columns, we introduce examples of ultra-high-speed analysis and ultra-high-resolution analysis of nucleic acid-related compounds.
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30% Applications Using "Prominence RF-20AXS" Fluorescence Detector (Part 9)Analysis of Aflatoxin 1, B2, G1 and G2 at High Sensitivity
Here, we introduce two cases of analysis of these 4 aflatoxins (B1, B2, G1, G2), using the Prominence RF-20AXS high-sensitivity fluorescence detector, first by fluorescence detection using trifluoroacetic acid derivatization, and then direct detection without TFA derivatization.
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30% iPad: Increasing the Productivity of HPLC Systems
The advantage of HPLC system controller web server functionality over workstation networking is the sweeping ability to assess multiple systems on the same network. Combining this feature with the swift startup and portability of the iPad provides even more merits as outlined below.
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30% High Speed, High Resolution Analysis (Part 44)Analysis of Pre-Column Derivatized Amino Acids by SIL-30AC Autosampler
Here, using the RF-20Axs Fluorescence detector and the SIL-30AC autosampler with its automated pretreatment functions, we introduce the analysis of amino acids using pre-column derivatization with OPA and FMOC.
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30% High Speed, High Resolution Analysis (Part 45) Analysis of Pre-Column Derivatized Amino Acids by the Nexera SIL-30AC Autosampler (Part 2)
Here, we introduce an example of the determination of 26 amino acids using a different column size and different mobile phase conditions than were used in L432. The automatic pretreatment feature of the SIL-30AC was utilized for derivatization of the amino acids during analysis, thereby enabling the overall analysis time to be substantially shortened.
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30% Study of an Efficient Method Development and Analysis Preparation Process using UHPLC
An actual analytical HPLC workflow requires time and labor in order to optimize analytical conditions. In this presentation, different processes, including automatic solvent blending and method scouting, as well as the Nexera Quaternary System, are presented, with an emphasis on improving the method development procedure.
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30% Ultra-high Speed Analysis of Ibuprofen within USP <621> Allowed Limits by Nexera Method Scouting
This application note shows an example of Ibuprofen analysis from the USP-NF. This analysis was based on USP <621> and done by using the Nexera Method Scouting System, with a conventional column, Shim-pack VPODS, and a core-shell column, Kinetex XBC18.
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30% Ultra-Fast Impurity Analysis Using a New High Sensitivity UHPLC Detector
Recently, a new high-sensitivity detector was introduced that provides improved UHPLC system performance with new features that improve workplace efficiency. Results presented will demonstrate the increased sensitivity of this detector. In addition, examples of peak deconvolution capabilities and the detector’s extended dynamic range will be presented.
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30% High Sensitivity Glycan Profiling in Antibody Drugs using UHPLC and Fluorescence Detection
Non-uniformity of glycans due to the instability of culture conditions used for antibody drugs is a concern which requires rigorous management of the production process. While there is currently no glycan test method specified in the U.S. Pharmacopeia, there is wide demand for such an assessment method. This poster introduces an example of UHPLC analysis of antibody drug glycans.
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30% Techniques for Reducing the Effects of Sample Solvent on UHPLC Analyses
For HPLC, sample solvents that adequately dissolve target compounds are required. Therefore, sample solvents that contain a high concentration of organic solvent are often used for reversed phase chromatography. However, these solvents sometimes cause peak broadening. This poster presents techniques to reduce the effects of sample solvents. Recent widespread UHPLC analyses are taken as examples.
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30% Preparative Separation of Active Components from Natural Products using Low-pressure Gradient Preparative HPLC
Natural products contain a variety of active components, and preparative HPLC is used to separate active components from complex mixtures such as natural products. This poster presents the preparative separation of rosemary using the Prominence low-pressure gradient preparative HPLC system.
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30% Determination of Carbamate Pesticides by APCI LC/MS
Carbamate pesticides have been used in agricultural pest control in the United States since the 1960s, as contact insecticides. Some have previously been used therapeutically as pediculocides and ectoparasiticides for humans and animals, respectively.
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30% Oil Despersants: Dioctylsulfosuccinate, 2-Butoxyethanol, and Dipropylene Glycol t-Butyl Ether
A rapid LC-MS-MS method for detection of oil dispersants in water was developed.
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30% Analysis of Haloacetic Acids in Tap Water Using LC/MS/MS [LCMS-8030]
This Application News introduces an example of analysis of the 3 haloacetic acids using the LCMS-8030 triple quadrupole mass spectrometer and is in accordance with this legislative measure.
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30% Fast LC-MS/MS Screening Method for Antipsychotics
A fast screening method for a group of typical and atypical antipsychotics has been developed which incorporates the acquisition of full scan MS and data dependent product ion spectra to screen for any untargeted analytes.
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28% Increase Your Productivity with our State-of-the-Art Autosamplers
Increase Your Productivity with our State of the Art Autosamplers
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28% Analysis of Drug Degradants by LC/MS
This Application News introduces an example of highspeed analysis of drug degradants by LC/MS. The ultra fast features of the LCMS-2020, scan rates up to 15,000 u/sec and positive/negative polarity switching as fast as 15 msec, were used in combination with in-source CID (Collision Induced Dissociation) to produce experimental results. In addition, formula prediction was conducted using the LCMS-IT-TOF, and separation of the sample components was carried out using the Prominence UFLCXR.
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28% Simultaneous measurement of positive-negative Ions of 30 pesticides for water management using the LCMS-8030 triple quad mass spectrometer.
Japan’s Ministry of Health, Labour and Welfare has designated simultaneous analysis using C18 solid-phase extraction and LC/MS to be the official analytical method for managing targeted pesticides in water (revised on April, 2008). Currently, 31 pesticides are covered. This document describes simultaneous MRM analysis using polarity switching with electrospray ionization (ESI) and LC conditions for 30 of these targeted pesticides (excludes Oxine-Cu). The LCMS-8030 is capable of setting the MRM measurement time of each compound independently. In addition, the polarity switching time is short (15msec), making the LCMS- 8030 suitable for simultaneous analysis of multiple compounds that ionize in both positive and negative modes.
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28% Quantification of Acetylsalicylic Acid and ist Related Substance Salicylic Acid
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28% A Rapid Method for the Determination of Amiodarone and ist Metabolite Desethylamiodarone
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28% Separation of Tricyclic Antidepressants Using Pathfinder Columns at Acidic Conditions
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28% Determination of Cimetidine in Pharmaceutical Tablets by High Performance Liquid Chromatograpy
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28% Mapping the Hydrophobic Character of Pathfinder Phases
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28% A Simple HPLC Method for the Detection of Preservatives in Inks
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28% How Hazardous is your Shampoo?
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28% Steric Selectivity of Pathfinder Columns According to Tanaka and Enhelhardt
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28% Controlling Selectivity by Means of Temperature in Reversed Phase Chromatography
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28% Simultaneous Determination of Thirteen UV Filters in Sunscreen Products
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28% Analysis of Pesticides Using the Triple Quadrupole LC/MS/MS (LCMS-8030)
According to the list of water quality control substances specified by the Ministry of Health,Labour and Welfare (April, 2010 revision), 31 pesticides are now included as items subject to analysis by "Method 18 - Simultaneous Analysis bySolid-PhaseExtraction-Liquid Chromatography/Mass Spectrometry." Here we conducted analysis of a mixture of 30 of these substances, excluding oxine-copper, but with the addition of Shimazine and Tiobencarb, using LC conditions based on the official method. MRM measurement was conducted by ESI (Electrospray Ionization) using positive-negative switching (Fig. 1). A triple quadrupole liquid chromatograph-mass spectrometer offers excellent sensitivity and selectivity in analysis of complex samples. Setting of the optimum parameters for Multiple-Reaction Monitoring (MRM) transitions is important for obtaining this excellent sensitivity and selectivity.
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28% Analysis of Geniposidic Acid and Chlorogenic Acid in Tochu Tea Using a Triple Quadrupole LC/MS/MS [LCMS-8030]
Tochu tea made using tochu (Eucommia ulmoides) leaves is well known as one of the 缀ve great traditional Chinese medicines. Tochu tea leaves contain abundant amounts of geniposidic acid (an iridoid glucoside) and chlorogenic acid (3-caffeoylquinic acid). Geniposidic acid is known to possess several pharmacological functions, one of which is the ability to reduce blood pressure. LC/MS is widely used for the analysis of these types of polyphenols in plant extracts. Here, we introduce an analysis of geniposidic acid and chlorogenic acid in tochu tea using a triple quadrupole mass spectrometer.
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28% Anion Analysis of Ophthalmic Irrigation Solutions with the Shimadzu Prominence IC System
Ophthalmic solutions suitable for eye irrigation, eye strain relief, contact lens storage and tear replacement abound in the marketplace. While some of these serve as convenient agents for administering medicines in minute quantity, many are formulated to act as cleaning agents and may contain only dissociated ions like chloride or sulfate and organic preservatives such as benzalkonium chloride. The physiologic pH of blood and tears is approximately 7.4, but a pH range of 6.5 to 8.5 will prevent damage to the cornea. This is accomplished using buffers. To prevent eye tissue damage and maximize comfort, solutions are made to be iso-osmotic (equal osmotic pressure) with tears. The common term for this is isotonic. A 0.9% NaCl solution in pure water is said to be isotonic with tears. In this application note we will aid in the quality control of such ophthalmic solutions for their content of common anions.
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28% Detection of Non-Separated Impurities by i-PDeA
When optimizing the separation conditions to be used for quantitation and fractionation of the principal components of a sample, the goal is to produce a reliable separation between the main components and impur i t ies , including minor co-component s . A photodiode array detector is useful for confirming the presence of unexpected impurities and co-components because each has a different spectral pattern. By using i-PDeA (Intelligent Peak Deconvolution Analysis), the UV spectrum obtained by the photodiode array detector is derivatized to remove the principal components so that only the impurities remain visible in the data trace. It is a function that permits more reliable detection of impurities than that offered by the conventional peak purity method, and allows efficient optimization of the separation conditions. Here, we introduce the detection and resolution of unseparated peaks using the high-sensitivity SPD-M30A photodiode array detector in conjunction with the i-PDeA function of LabSolutions.
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28% High Speed, High Resolution Analysis (Part 46) Analysis of Pre-Column Derivatized Biogenic Amines by the Nexera SIL-30AC Autosampler
Biogenic amines are produced naturally by the enzymatic decarboxylation of amino acids in beverages and food. Biogenic amines such as these are also used as an indicator of food spoilage. Histamine, a substance that can cause allergy-like food poisoning, must not exceed 50 ppm in food in general according to FDA standards, 100 ppm in marine products in the EU, and 400 ppm in fish sauce according to the Codex International Food Standards. In addition, biogenic amines such as cadaverine and tyramine appear to intensify the allergy-like food toxicity of histamine. In Application News articles L432 and L437, we introduced examples of the pretreatment functions of the SIL-30AC autosampler in the analysis of fluorescence-derivatized amino acids us ing o-phthalaldehyde (OPA). Here, we introduce an example of the analysis of fluorescent amines that were derivatized with OPA.
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25% LC/MS Analysis of Perfluorochemicals (PFCs) Using Impurity Delay Method
Using the LCMS-2020 with its excellent sensitivity and repeatability and its newly developed QoQ ion optical system, together with the impurity delay method, allows 0.1 μg/L trace-level quantitative analysis of PFOA and PFOS with excellent repeatability.
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25% Impurity Analysis of Highly-Polar Compounds Without Using Ion-Pair Reagents
Ion-pair reagents which contain fluorine are often used for LC/MS; however, as ion-pair reagents tend to accumulate in the column, interface, switching to another separation mode may take a considerable amount of time to allow complete flushing of the residual ion-pair reagents. Here we introduce analytical conditions that do not include the use of an ion-pair reagent.
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25% Analysis of Methylglyoxal-Modified Heat Shock Protein 27 Using C-MALDI System
In this application we introduce the results of analysis of MG modification of the heat shock protein Hsp27 in response to various stresses (acidification, chemical substances, etc.) using the Prominence nano-AccuSpot-AXIMA® Performance LC-MALDI system. It is notable that several new modifications were found during this experiment.
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25% Development of a LC-QIT-TOF mass spectrometer capable of high mass accuracy and stability.
The combination of QIT-TOF with an ESI source has been developed using novel ion introduction optics. Instrument performance characteristics have now been extensively investigated in regards to mass accuracy and stability.
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25% Global Metabolite Profiling of Human Urine using LC-MS on a new Ion-Trap Time of Flight hybrid Mass Spectrometer
In this study the pooled QA sample was used to characterize the reproducibility of the system. Using selected peaks as QC markers, the CV for peak area was typically less than 10% throughout the sample analysis. This approach results in greater confidence in interpreting statistical analysis and can be used to identify components of biological interest with higher certainty.
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25% Fractionation of Anthocyanins by Preparative LC-MS System
Anthocyanins are the flavonoid color pigments present in all tissues of higher plants, including the petals and leaves.Besides their use as food dyes, anthocyanins have recently attracted attention due to their antioxidant properties.
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25% Analysis of Polymer Additives Using the LCMS-8030 Triple Quadrupole LC/MS/MS
Antioxidants, ultraviolet absorbers, flame retardants and other polymer additives are indispensable for improving the characteristics and functionality of polymers. Understanding the types and quantities of these additives to be used is extremely important to effectively control the manufacturing process of the polymers. Below is an example of quantitative analysis of 14 types of polymer additives using MRM (Multiple Reaction Monitoring) and the LCMS-8030 triple quadrupole mass spectrometer.
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25% Analysis of Serotonin using LC-MS-MS
Serotonin is an important neurotransmitter responsible for feelings of mood and cognitive functions, sleep, and appetite. Many pharma-ceuticals target pathways related to serotonin. The structure of serotonin is shown in Figure 1. Analytical methods to measure serotonin are needed to study the effects of this important neurotransmitter.
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25% Qualitative Analysis of Tochu Tea Using a Triple Quadrupole LC/MS/MS [LCMS-8030]
The LCMS-8030 triple quadrupole mass spectrometer is used in qualitative analysis to conduct precursor ion scans to search for precursor ions that produce common product ions, and to conduct neutral loss scans to detect ions that dissociate to common neutral fragments. Here, we describe the precursor ion scan in the search for active ingredients in Tochu tea using the LCMS-8030.
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25% Analysis of 3-MCPD Fatty Acid Diesters in Palm Oil Using a Triple Quadrupole LC/MS/MS [LCMS-8030]
This application note illustrates that it is possible to use a triple quadrupole mass spectrometer for detection of 3-MCPD fatty acid esters using a simple pretreatment procedure that is limited to sample dilution.
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25% Analysis of Perchlorate in Water by Non-Suppressed Ion Chromatography-Mass Spectrometry
Perchlorate is a chemical substance that occurs both naturally and as a manufactured compound, and is used in a wide range of applications. It is a common accelerant in rocket engines and an explosive in the pyrotechnics industry. From the standpoint of health, however, it is now suspected to be associated with hypothyroidism. A non-suppressed single LC/MS system is a low-cost, easy-to-operate system for monitoring perchlorate in water such as factory wastewater.
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25% High Throughput LC-MS-MS Analysis of Carbendazim in Orange Juice
Highly sensitive analytical methods are necessary to prevent contaminated produce from entering the food supply. This poster showcases the development of an ultrafast UHPLC-MS-MS method for analysis of carbendazim in orange juice using a new high-speed UHPLC autosampler in tandem with a newly developed high-sensitivity triple quadrupole mass spectrometer.
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25% Analysis of Hemoglobin Variants using an Automated Sample Preparation Workstation Coupled to LC-MS
There are over 2000 known hemoglobin variants, and it is important to be able to structurally identify and quantitate them quickly and precisely. This poster presents a quick, reproducible method of identifying differences between normal and abnormal hemoglobin utilizing an automated sample preparation system, which reduces sample complexity, coupled to mass spectrometry.
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25% Application of Metabolomics Techniques using LCMS and GCMS Profiling Analysis of Green Tea Leaves
This Application Note presents the profiling of green tea leaves as an example of one application of the metabolome analysis technique using LC/MS and GC/MS. A search of the substances believed to play roles in product quality was conducted, and a quality prediction model was constructed based on the results.
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25% Application of HPLC to the Analysis of Cosmetics
For the analysis of antiseptics and disinfectants contained in cosmetics, applicable methods of analysis are GC, UV-Vs spectrophotometry and HPLC.
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25% Analysis of Ointment
By using HPLC, active ingredients in products can be identified and determined in a short time, only by a very simple pretreatment.
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25% Applications of HPLC to Pharmacognosy
Active ingredients in crude drugs vary in contents depending on the sampling time and place of origin, and the qualty evaluation of the crude drugs is determined by the contents of the ingredients.
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25% Applications in Food Analysis (16)
The fungicide methods shown here, to protect plants and fruit, were adopted from the reversed phase ion-pair chromatographic technique for these substances
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25% Application of Preparative Liquid Chromatography (3)
In order to successfully conduct a recycle separation, it is necessary to reduce extra-column band broadening.
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25% Proposal to Reinforce Forensic Laboratories
In scientific criminal search and study, miscellaneous techniques are used for ultra high
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25% Analysis of Hair Tonics
Shown in this application note is a chromatogram of Hinokitiol standard, and one in hair tonic. In this analysis, the hair tonic was diluted with methanol and a sample of it was injected into the HPLC for analysis.
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25% Computer-assisted Optimizatiuon in the Development of a HPLC method for the analysis for Amines
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25% Column Classification and Selection of Pathfinder stationary phases with embedded Polar Groups
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25% Realization of HPLC Methods by using Computer Assisted Method Development
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25% Separation of Nucleotide Acid Bases with Reversed Phase Chromatography under Aqueous Mobile Phase Conditions
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25% Peptide Separation Using a Polymer Encapsulated Reversed Phase Column
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25% Analysis of Analgesics by UFLC
This application note focuses on the high-speed analysis of over-the-counter analgesics with Ultra Fast HPLC.
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25% Efficient separation and accurate spotting of NBS Labeled Peptides Using the Micro HPLC AccuSpot System
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25% Analysis of Golf Course Pesticides Using Triple Quadrupole LC/MS/MS (LCMS-8030)
Many kinds of pesticides are used to maintain golf course turf, and because golf courses are often located in mountain corridors and near water sources, pollution of nearby fresh water sources and tap water from golf course waste water is of concern. With this increased concern over the environment, in 1990 Japan's Ministry of the Environment issued the "Provisional Guidance Indicators for the Prevention of Water Pollution from Pesticides Used in Golf Courses" (hereafter, Guidance Indicators), in which indicator values were specified for 21 pesticides. Then, a notification issued on September 29, 2010 increased the list to 72 pesticides and made it a requirement to conduct simultaneous analysis of the chemicals using higher sensitivity instruments. Of those 72 pesticides, we conducted simultaneous analysis of 21 of the remaining 44 substances specified to be evaluated by LC/MS using the LCMS-8030, and in addition, we conducted monitor testing of golf course wastewater. The results are presented here.
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24% Applications of the Prominence RF-20AXS - Fluorescence Detector - High Sensitivity Analysis of Anionic Surfactants, tert-Butylhydroquinone, and Amitrole
The Prominence RF-20AXS fluorescence detector not only achieves high sensitivity levels never before seen (water Raman SN ratio greater than 2000), but it also features a temperature-controlled flowcell with a cooling function as standard to provide highly reliable detection unaffected by ambient temperature fluctuations. Here we introduce examples of high sensitivity analysis using the Prominence RF-20AXS.
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24% Applications of the "Prominence RF-20Axs" Fluorescence Detector (Part 4) Analysis of Pesticides with a Postcolumn Derivatization System
Fluorescence detectors are widely used in the food and environmental fields due to their high sensitivity and selectivity. Among these applications, fluorescence detection is used for analysis of iminoctadine and Nmethyl carbamate pesticides in food and tap water by a post-column fluorescence derivatization method. Here we introduce examples of these analyses using the Prominence RF-20Axs fluorescence detector with the Prominence Iminoctadine Analysis System and the Prominence Carbamate Analysis System.
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24% Analysis of Ginkgolic Acids in Ginkgo Biloba Extract
Ginkgo leaf extract (ginkgo biloba extract), which contains active ingredients extracted from ginkgo biloba leaves, is reported to be effective in improving cerebral and peripheral blood circulation deficiencies. It is used in Japan and the United States in the form of a dietary supplement, and in Germany, France, and other European countries as a prescription medication. However, alkylphenols which are present in the ginkgolic acids contained in ginkgo leaves are known to cause allergic reactions. For this reason, the United States Pharmacopeia (USP) has established an upper limit for ginkgolic acid content in ginkgo biloba extract. Here we introduce an example of analysis of gingkolic acids contained in ginkgo biloba leaves.
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24% High Speed, High Resolution Analysis (Part 31) Analysis of Mexiletine, Pilsicainide, and Zonisamide in Serum
HPLC separation and quantitation are often used for measurement of drug concentrations in the blood. When analyzing the concentration of a drug in the blood using HPLC, the separation of contaminants and analytes requires a long analysis time. For that reason, there is demand for faster HPLC analysis to improve the productivity of drug pharmacokinetic studies and clinical testing, particularly when handling many samples. Here we introduce analyses of mexiletine, pilsicainide, and zonisamide in serum using the ultra-high speed Prominence UFLC system and high-speed Shim-pack XR-ODS separation column.
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24% Applications Using "Prominence RF-20Axs" Fluorescence Detector (Part 6)
Fluorescence detectors are widely used in the environmental analysis due to their high sensitivity and high selectivity. Even trace amounts of chemical compounds often affect the ecosystem. Bisphenol A is one of such compounds for which trace-level analysis of environmental water is greately required. Here we introduce examples of high-sensitivity analysis of bisphenol A using the Prominence RF- 20Axs fluorescence detector by direct injection and by using an automated column-switching preconcentrating system.
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24% Internal Standard Addition During Pretreatment Using SIL-30AC
Data with excellent repeatability was obtained using both the internal standard "stacked injection" and the internal standard mixing and dilution pretreatment methods. The examples presented here focused on the use of the internal standard addition technique, but in the amino acid analysis example as well, the pretreatment program was utilized to demonstrate that good repeatability can be obtained without concern for even slight differences in reaction time. In particular, the more complicated the pretreatment process, the more difficult it becomes to maintain repeatability when conducting pretreatment manually. For this reason, the Nexera SIL-30AC pretreatment functions were designed to address a wide range of pretreatment processes, from automated primary screening of large numbers of samples to pretreatment requiring accurate reaction time.
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24% Analysis of Hexafluorophosphate Ionin Electrolytic Solution for Lithium-Ion Rechargeable Battery
In Application News No. L403, we introduced an example of the analysis of electrolytic solvents used in lithium-ion rechargeable batteries. Here, we will investigate the analysis of electrolytes. In order for a substance to be suitable as an electrolyte, it must dissociate in a non-aqueous solvent and be able to withstand the oxidation-reduction environment at the cathode and anode. One such typical electrolyte is lithium hexafluorophosphate. Here, we introduce an example of analysis of the hexafluorophosphate ion in the electrolytic solution used in lithium-ion rechargeable batteries by HPLC.
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24% Applications Using "Prominence RF-20Axs" Fluorescence Detector (Part 6) - Analysis of Bisphenol A at High Sensitivity
Fluorescence detectors are widely used in the environmental analysis due to their high sensitivity and high selectivity. Even trace amounts of chemical compounds often affect the ecosystem. Bisphenol A is one of such compounds for which trace-level analysis of environmental water is greatly required. Here we introduce examples of high-sensitivity analysis of bisphenol A using the Prominence RF-20Axs fluorescence detector by direct injection and by using an automated column-switching pre-concentrating system.
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24% Applications of the Prominence RF-20AXS Fluorescence Detector (Part 7)High Sensitivity Analysis of Retinol Acetate and Retinol Palmitate
In everyday use, the term "Vitamin A" is synonymous with retinol. Because retinol is a substance that is easily oxidized, highly stable derivatives of retinol are used in such products as foods, medicines and cosmetics. In HPLC analysis of stable retinol derivatives such as retinol acetate and retinol palmitate, a fluorescence detector offers both selectivity as well as high-sensitivity analysis. Here we introduce examples of high-sensitivity analysis of retinol acetate and retinol palmitate, in addition to ultra-high speed analysis of retinol palmitate using the Prominence RF-20AXS fluorescence detector.
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24% Performance Comparison of Variable Wavelength and Photodiode Array Detectors
Shimadzu strives to achieve superior detector performance by designing high quality optical benches. To achieve this in both the UV and Vis range, dual lamp detector design is offered for both variable wavelength and PDA models. The SPD-M10A and SPD-M10AV PDA detectors demonstrate comparable UV sensitivity. Both the SPD- M10A and M10AV are approximately one and one-half times higher in sensitivity than the SPD-M6A; and, the SPD- 10AV UV-Vis detector demonstrates six times more sensitivity than its photodiode array counterparts.
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24% Analysis of Ointment
Lotions and ointments are common household items. Ointments are used to relieve itching and inflammation, treat eczema, cure athlete's foot, and for hemorrhoid therapy. Spectrophotometric and acid-base titration methods have been conventionally used to analyze these medicines, but this requires complex sample preparation. Faster identification of active ingredients is achieved using HPLC, with only pretreatment. The analysis of the active ingredients contained in medicines for skin disorders (e.g. impetigo, eczema), analgesic treatments (e.g. arthritis, insect bites, etc.), and anti-fungal medicines (e.g. athlete's foot, ringworm, etc.) are presented in the following chromatograms. Samples were provided be Belbe Chemical Industries, Co. Ltd., Japan.
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24% GPC Analysis of a Non-Ionic Surfactant Mixture
Generally, when analyzing surfactants by GPC, the length of the lipophilic group determines the separation. When surfactant mixtures are analyzed by single-column GPC, the peaks often overlap, creating identification difficulties. This Application Note describes the analysis of surfactants by combining two columns. The Shimadzu Shim-pack GPC column, packed with hydrophobic gel, was used in a series with a Shimadzu Asahipak GS hydrophilic gel column. An injection was made of a mixture of an ester (A), POE (B), and amide (C) type of non-ionic surfactants.
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24% GPC Analysis of Polymide, Polyamic Acid and 12-Nylon
The GPC analysis of polymers depends upon solvent(s) that can completely dissolve the target polymer. These solvents also serve as the mobile phase in GPC analysis. If an appropriate solvent cannot be found, analyses will not provide accurate molecular weight distribution data. With the development of GPC columns, a variety of solvents (other than the widely used THF and chloroform) can now be used. This greatly increases the type of polymers that can be analyzed by GPC. Examples of the anallysis of polyimide, polyamic acid, and nylon-12 are given below.
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24% Cookie Cutter Proteolysis: Achieving Reproducible, Efficient Digestions for Proteomic Workflows
Protein sample preparation workflows for mass spectrometric analysis that involve proteolysis are often labor-intensive, time consuming and user dependent. These workflows often involve digestion, solid phase extraction, drying, and re-suspension prior to reversed phase separation into the mass spectrometer. The introduction of variability at many of these steps hinders discovery initiatives as well as the ability to convert these discoveries into viable assays. Recently, an automated protein digestion platform was developed which accelerates the digestion process through use of immobilized enzyme reaction (IMER) columns. Furthermore, online digestion is integrated with desalting and reversed phase chromatography to create a seamless workflow to the mass spectrometer once the sample is injected. The benefit of streamlining these processes can be demonstrated by the reproducibility achieved.
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24% A Modified INA Method for the Analysis of Milk Thistle Using
One of the widely available nutraceutical products seen in supermarkets is a powdered form of Milk Thistle or silybum marianum, derived from the plant native to Mediterranean Europe. It has been used from ancient times as a liver tonic. The milky sap produced by Milk Thistle contains flavolignans, collectively known as the silymarin group. Growing consumer interest, combined with the low cost and growing availability of herbal supplements and remedies, has driven the need for standardization of these products.
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24% The Perfinity Workstation: Achieving Quality through Automation
The Perfinity Workstation effectively removes the sample preparation bottleneck by automating and integrating reproducible and rapid affinity selection, sample clean-up, proteolysis and buffer exchange. All of these processes can be completed in under 10 minutes, resulting in peptide maps of exceptional reproducibility (<10% CVs). Applications of this workstation include targeted proteomics, biomarker validation and PTM analysis.The Perfinity Workstation incorporates software that is extremely easy to use, greatly reduces the learning curve and requires no method programming. The workstation allows everyday users to apply MS to proteins, integrates with current proteomics/biomarker MS workflows, dramatically reduces sample prep time, is fully automated, multiplex capable, easily customizable and requires no antibody immobilization. More information is available at Perfinity.com and PerfinityWorkstation.com.
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24% Analysis of Dye Degradation in Dye-Sensitized Solar Cell
Of the organic photovoltaic cells that have been under extensive development as next-generation solar cells, dye-sensitized solar cells are perhaps the closest to commercialization today. These types of solar cells are expected to be produced inexpensively. Commercialization will of course be contingent on sufficient photoelectric conversion efficiency, but equally important will be their endurance in withstanding degradation over time. By using HPLC, it is possible to observe changes in these dyes over time. Here we introduce some analytical examples of the degradation of one such dye, referred to as N3 dye.
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24% Shimadzu at MSACL 2012 Workshop

Prepare Your Lab for the Future…
From Automated Sample Prep to Ultrafast Mass Spectrometry

Presented at The Association for Mass Spectrometry Applications to the Clinical Lab (MSACL) 2012 conference, this workshop consists of the following presentations:

– Achieving Truly Ultrafast UHPLC-MS-MS, Jeff Dahl, Shimadzu Scientific Instruments
– The Perfinity Workstation: A Faster, More Reproducible, Fully-automated Sample Prep Platform, Kevin Meyer, Perfinity Biosciences
– Integrating the Perfinity Workstation into the Biomarker Validation Pipeline for Diagnostic Assays, Christine Jelinek, Johns Hopkins School of Medicine

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24% Analysis of the Decomposition Products of Lithium Hexafluorophosphate in the Electrolytic Solution of Lithium-Ion Rechargeable Batteries by Column-Switching Ion Chromatography (Part 2)
Lithium hexafluorophosphate is commonly used as the electrolytic solution in lithium-ion rechargeable batteries. It is hydrolyzed by the small amounts of water contained in the electrolytic solution to produce fluoride and other ions. In Application News L417, we presented an example of analysis of electrolytic solution which had deteriorated due to exposure to air, using a column-switching ion chromatography system. Here, we introduce an analysis of an electrolytic solution that had been subjected to accelerated deterioration during testing.
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24% Increasing the Throughput of UHPLC
UHPLC has proven to be an effective way to reduce analysis times without losing separation efficiency through the use of small particle and core-shell column technologies. The use of higher column temperatures and shorter column lengths has allowed the analysis speed of UHPLC to be further increased. A number of high-speed UHPLC applications and conditions will be presented which now allow up to four analytical runs to be completed in only a one-minute timeframe.
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24% High Speed Analysis of Mycotoxins
Mycotoxins are low-molecular-weight natural products produced by fungi and are capable of causing disease and death. Mycotoxins are typically found in grain products and their occurrence is increased under certain heat and humidity conditions. Regulatory levels have been set for mycotoxin levels, and are dependent on the end use of the food products. Mycotoxin levels can vary widely within the same crop, creating a potential need for increased testing to provide more accurate mycotoxin levels. A high-speed UHPLC method was developed that could provide separation of eight common mycotoxins in less than two minutes to allow for increased testing. Details of the method will be presented.
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24% Auto Dilution of Standard and Sample Solutions Using the SIL-30AC Autosampler
Pretreatment operations were shown to require less time and effort when using the automatic dilution feature of the SIL-30AC. Furthermore, these automated functions allow highly accurate results, free of technician-related errors. When using the SIL-30AC, automatic dilution and automatic reagent addition can easily be set up by using standard templates. Also, use of pretreatment programs makes it possible to set up very complex operations for a wide range of functions to be executed automatically.
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24% Using a Phenyl Column When Separation with C18 (ODS) Is Insufficient
The C18 column is an ODS type of column that is marketed by instrument and column manufacturers, in which octadecyl groups are bonded to a silica base to provide wide separation applicability. An appropriate ODS column is typically the first type selected from the various types available at the start of a reverse-phase analysis. However, in the process of investigating the mobile phase conditions, there are cases in which the separation conditions cannot be improved. Selecting a different ODS column or exchanging it with a C8 column to improve separation is also possible, but even then, it may not be possible to achieve sufficient separation.
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24% Determination of Drugs in Serum using Heart-cutting Two-dimensional Ultra High Performance Liquid Chromatography
HPLC separation and quantitation are often used for measurement of drug concentrations in the blood. When analyzing the concentration of a drug in blood using HPLC, it is necessary to separate analytes from other compounds. However, the separation of analytes and other compounds in blood is not easy because of the complex matrix. Sample preparation is useful for these cases to reduce matrix, but it can take a long time. In this poster, we analyzed propranolol in serum using heartcutting two-dimensional ultra high performance liquid chromatography. This method was developed on our UHPLC system equipped with a high-pressure switching valve.
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24% Fractional Determination of Co-eluted Compounds Using a New Data Processing Method for Photodiode Array Detector
The i-PDeA derivative spectrum chromatogram method was developed as a new data processing technique for photodiode array detectors for HPLC. A derivative spectrum is created by performing differential processing on the UV-Vis absorption spectrum at each measurement time. Plotting the derivative spectrum values at the specified wavelength against retention time creates a derivative spectrum chromatogram that is able to separate co-eluted peaks. The high selectivity of the derivative spectrum chromatogram can detect unexpected impurities and quantitate the target component only, without effects from interfering components that elute simultaneously. This paper formulates the theory of the derivative spectrum chromatogram method into mathematical expressions and reports details of verification of the basic performance using standard samples.
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21% New Data Processing Method for Photodiode Array Detector
A new data processing method for a photo diode array (PDA) detector, Intelligent Dynamic Range Extension Calculator (i-DReC) enables the automatic calculation of peak area and height, utilizing spectrum similarity in the high concentration range where UV signal is saturated. When the integrated chromatographic peak area exceeds a user-defined threshold value, i-DReC automatically shifts the chromatographic profile to a wavelength with less UV absorption to prevent signal saturation. The absorption ratio between the original target wavelength and the wavelength used by the i-DReC function is applied as a correction factor to the peak area of the acquired chromatogram, thereby calculating the peak area and height at the original target wavelength. The i-DReC dramatically extends the linear dynamic range of calibration curves, enabling reliable quantitation of high concentration samples without need for sample dilution and reinjection, which would otherwise be required.
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21% Four-Channel Vacuum Degasser-Maximum Degassing Efficiency in an Ultra-Compact Design.
Eliminates helium degassing to save time and money/Fast, efficient degassing from up to 4 solvent channels/Steady, Self-Maintaining Vacuum/Metal-Free Bioinert Composition and Advanced Self-Diagnostics
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21% CLASS-VPTM - New Tools for Better Solutions
CLASS-VPTM is a multi-tasking chromatography data acquisition and instrument control system operating under Microsoft WindowsTM.
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21% CLASS-VPTM - New Tools for Better Solutions
A New Program with New Tools, to Make Chromatographic Data Processing More Powerful Than Ever acquisition and instrument control system operating under Microsoft WindowsTM.
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21% TOGAS - Transformer Oil Gas Analysis System
The analysis of gas in transformer oil is not a new requirement. Care and maintenance of expensive transformers has been facilitated by routinely sampling the transformer oil and determining the concentration of various gases.
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21% Vitamin Analysis with an Integrated HPLC System
In the last few years, vitamin supplements have become increasingly important to consumers who are concerned about maintaining the recommended daily allowances of certain vitamins, or who are focusing on specific vitamins that show promise of disease prevention or other health benefits.
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21% High Throughput Analysis of Anti-Epileptic Drugs
Epilepsy is a neurological disorder characterized by varying intensity of seizures. The exact cause of epilepsy is unknown, but any kind of trauma to the brain such as head injury, high fever, large doses of drugs and other toxic substances, or interruption of blood flow to the brain can contribute to seizures.
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21% Steroid Analysis with an Integrated HPLC System
Steroids are a structurally unique type of drug, most commonly used for anti-inflammatory purposes. In general, they can be used to treat asthma, arthritis, allergies, and may be combined with other medications to help prevent the body from rejecting transplanted organs.
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21% Aromatic Acid Analysis with an Integrated HPLC System
Aromatic acids are derivatives of phenol and have a wide range of practical uses. Compounds such as benzoic acid and p-hydroxybenzoic acid are common preservatives in beverages, snack foods, and cereals. Others including salicylic acid and acetylsalicylic acid show analgesic, anti-inflammatory, and antiseptic properties.
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21% The HPLC Analysis of Underivatized Amino Acids
Because they lack suitable chromophores, the traditional approach to analyzing amino acids by HPLC is either pre- or post-column derivatization and detection by fluorescence. While this approach yields excellent sensitivity, the derivatization process is time consuming and may introduce variability from run to run.
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21% The HPLC Analysis of Carbohydrates
Carbohydrates from simple sugars to oligo- and poly-saccharides represent an HPLC detection challenge in that they are non-chromophoric. Traditional approaches to their detection, RI and UV (195 - 205nm), are problematic due to issues of poor sensitivity, long detector equilibration times and an inability to handle gradient elution.
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21% Analysis of Berberine in Crude Drugs
The SPD-M6A, employing a photodiode array sensor, is capable of storing data in the wavelength range of 195 nm to 870 nm, while observing the chromatogram at two wavelengths selected arbitraily in real time.
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21% Analysis of Polycyclic Aromatic Hydrocarbone with a Multi-Functional Fluorescence Detector
The multi-functional fluorescence detector RF-550, capable of setting or varying the excitation and fluorescence wavelength by the time program is a new tool useful in simultaneous analysis of plural components differing in the detection optimum wavelength.
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21% High Sensitivity and High Selectivity Analysis of Ammonia with HPLC.
Quantitative measurement of ammonia is being performed in a wide field, including quality control, measurement in working environment, monitoring of environmental atmosphere, as well as in the field of biochemistry.
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21% High Speed Analysis of Explosives
Showcasing the development and use of a high-speed reversed phase method for monitoring explosive levels in soil samples near current and former munition plants, an important objective for both environmental and safety reasons.
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20% Using the Shimadzu HPLC System in the Fuel-Grade Ethanol Production Laboratory
Liquid Chromatography (LC) is any chromatographic procedure in which the moving phase is a liquid. In most cases, a stationary phase is also necessary. High Performance Liquid Chromatography (HPLC) has become the most widely applicable method of chromatographic analysis. Through use of highly efficient column materials (the stationary phase), pumping systems that provide a consistent flow of the mobile phase, and detection components designed to accommodate the needs of the analysis, HPLC is now a commonly used technique for monitoring the progress of the fermentation in the ethanol production laboratory.
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20% LCMS-IT-TOF Analysis of Amoxicillin
Amoxicillin was successfully separated and detected under gradient conditions using a Shimadzu Prominence series LC coupled to the LCMS-IT-TOF. Data acquired on the LCMS-IT-TOF allows for MSn and excellent mass accuracy within one experiment. Mass accuracy data obtained from the LCMS-IT-TOF is comparable to data reported by other vendors requiring the use of an internal standard or a dual-sprayer configuration.
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20% Analysis of PFCs in Environmental Water Using Triple Quadrupole LC/MS/MS (LCMS-8030)
Organofuorine compounds such as perfuorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) are highly stable chemical compounds which are widely used as water repellents, oil repellents and coating agents. These compounds do not decompose easily in the natural world due to their stability. Reports of their detection in rivers, streams, tap water, food, as well as in the atmosphere and in human blood has led to concern of their effect on the human body as environmental pollutants. Here we conducted the simultaneous analysis of PFOA, PFOS, and related compounds using the LCMS-8030. The results of an environmental water monitoring study that we conducted are also presented.
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20% Analysis of Pesticides in Foods Using a Triple Quadrupole LC/MS/MS [LCMS-8030]
Pesticides are widely used in agricultural products to protect crops from insect infestation during cultivation and product transport. With the heightened concern for food safety, “Positive List System” was introduced in Japan on May 29, 2006. These limits ban the sale of food containing pesticides that exceed specified concentrations to ensure the safety of food circulating in the market. Currently, residue standards and analytical methods have been established for about 800 kinds of pesticides and veterinary pharmaceuticals, and this number is expected to increase even further. Therefore, there is increasing need for simpler analytical methods capable of simultaneous multi-residue analysis.Here, using the LCMS-8030, we optimized methods for the simultaneous multi-residue analysis of 43 pesticides, and report the results of the analysis of actual vegetable extracts (paprika and leek) in solution.
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18% High-Sensitivity Quantitative Analysis of Trace-Level Impurities and Active Ingredients with HPLC
As the trend toward smaller and smaller analyte concentrations progresses, conventional analytical methods are becoming less and less able to address trace-level analyses. For example, not only do product impurities affect product quality, there are increasing instances of product safety being compromised as well. This has prompted increasingly stringent impurity control across a wide range of business enterprises, where concern about quantitative analysis of extremely low levels of impurities is the current reality. On the other hand, when the analyte is the active ingredient itself, it becomes increasingly difficult to establish a quantitative method as the concentrations decrease to minute levels. While the typical approach to addressing this situation is to increase sensitivity by taking such measures as increasing the sample concentration, there is always the concern that the solubility of the principal ingredient may be adversely affected, or that the analyte might not be sufficiently separated from possibly large numbers of obstructing substances in the sample matrix (see Fig. 1). Addressing this situation often necessitates the use of pretreatment procedures such as extraction and concentration, which also adversely affects efficiency due to the extra time and labor involved in conducting complicated pretreatment procedures. Thus, it becomes necessary to satisfy two very different requirements for the quantitation of trace-levels of analytes: increasing measurement sensitivity and speeding up operations. The key point in achieving this is to automate the entire process from the fraction collection (fractionation) and concentration of the analytes to final quantitation.
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18% The Shimadzu ELSD-LT Evaporative Light Scattering Detector
Evaporative Light Scattering (ELS) detection has entered the HPLC detection mainstream in recent years. It is a valuable complement to UV, PDA and MS, but it is also a preferred detector for such applications as carbohydrates, phospholipids, triglycerides and surfactants. The ELSD makes a light scattering measurement of target analytes that have been dried of mobile phase through evaporation.
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18% Evaporative Light Scattering Detection as a Complement to UV for Flavor Profiling Peppers by HPLC
This data supports the use of 280nm UV for determining only the capsaicins as it is highly selective for those constituents. The ELSD-LT offers numerous advantages: better baseline response for the gradient method, and detection of all flavor constituents, both chromophoric and non-chromophoric, in one run.
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18% The HPLC Determination of Salicin and Polyphenolic Glucosides
Extract from the bark of the White or European Willow, salix alba, has been used as folk medicine for many years in treating inflammation, pain and fever. The principle component of the water extract from salix bark is salicin, 2-(hydroxymethyl)phenyl-â-D-glucopyranoside, a human-metabolic precursor to salicylic acid.
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18% GPC-MALDI Analysis of Trace Components in Synthetic Polymers
Matrix-Assisted Laser Desorption / Ionization Time-of-Flight mass spectrometry (MALDI-TOF MS) is being employed in a growing number of synthetic polymer applications in recent years. Using this technique, monomer and terminal structures are predicted based on the masses of the observed polymer substance, molecular weight distribution is evaluated, and molecular structural analysis is conducted by MS/MS analysis.
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18% Using the HPLC System in the Fuel-Grade Ethanol Production Laboratory
High Performance Liquid Chromatography (HPLC) has become the most widely applicable method of chromatographic analysis. Through use of highly efficient column materials (the stationary phase), pumping system that provide a constant flow of the mobile phase, and detection components designed to accommodate the needs of analysis, HPLC is now a commonly used technique for monitoring the progress of the fermentation in the ethanol production laboratory.
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18% Flash and Continuous Light Sources in Fluorescence
For fluorescence measurements from the ultraviolet through the visible and into the near infrared region, the typical light source is the Xenon arc lamp. This particular source has been widely used due to its favorable characteristics, primarily the ability to deliver high energy output over a wide wavelength range.
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17% Analysis of Malachite Green Using a Triple Quadrupole LC/MS/MS [LCMS-8030]
Malachite green, besides being used as a dye in the textile and paper industries in Japan, is also used as a synthetic antibacterial drug to treat diseases such as water mold disease in aquarium fish. Due to concern related to its carcinogenicity and genotoxicity, not to mention the persistence of its metabolite, leucomalachite green, application of malachite green with aquaculture animals is prohibited under the Pharmaceutical Affairs Act. The United States in 1981, and the European Union and China in 2002 prohibited its use with all food-related items. However, due to its low price, effectiveness, and easy availability, cases of its detection in eel, salmon and other farmed fish continue to appear, resulting in strengthened worldwide monitoring.Here, we show the quantitative analysis of malachite green and leucomalachite green using the LCMS-8030.In addition, we report the results of spiked-recovery measurements conducted using a salmon extract solution as an actual sample.
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14% Analysis of Polymer Additives in Synthetic Polymers using a SEC-MALDI System
Synthetic polymers may deteriorate as a result of various factors such as light, heat, mechanical stress, electrical stress, radiation, chemicals and moisture. Various additives such as antioxidants, UV absorbent, HALS and thermal stabilizers have been developed to maintain the properties of polymers and prevent degradation. The amount of additive depends on the intended use of the polymer but, typically, it is less than 1% (w/w). Analysis of additives in synthetic polymers is therefore important for the evaluation of improvement of polymer properties. Recently, MALDI-TOF MS has been used for the analysis of polymer additives.
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14% Agent Report: Limiting Report Creation Risks Through Automatic Integration of Various Types of Data
This report entails how to limit report creation risks through automatic integration of various types of data. This report covers ensuring reliability of computer-acquired data, risks to analysis and test data, data alteration, difficulty of handling different types of data, integrating different types of data with agent report, ensuring a high level of security and agent report flexibility and extendibility.
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