May 29 2012

Shimadzu Launches Improved Accurate Glycan Analyzer 2 for the Automated Analysis of Complex Branched Glycans


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Shimadzu Corporation launches a new improved Accurate Glycan Analyzer 2 (abbreviated as AGA2 in this document): a combination of biologically-relevant glycan database and the unique MSn properties of the AXIMA® Resonance.

Glycans play an important role in biological processes and are involved in protein interactions as well as cell surface recognition mechanisms. Despite this they have received little attention. This is in part due to the complex branched structure of the compounds which has meant that their study and structural identification has been a highly specialized discipline: Until now!

Combining the simplicity of MALDI, the power of MSn, and the accuracy and resolution of Time-Of Flight, the AXIMA® Resonance provides a unique solution for the next generation of complex structural and sequencing challenges. The AXIMA Resonance features high mass resolution, mass accuracy and sensitivity across MS and MSn experiments. High performance of multiple MSn analysis makes it ideal for the structural study of glycans.

The AXIMA Resonance is coupled to a database containing glycan structural information generated from actual MSn spectra of to select precursor peaks up to MS4. The resulting spectra are interpreted to return the most likely glycan structure.

The product is a result of collaboration between Shimadzu, the Advanced Industrial Science and Technology (AIST) and the Mitsui Knowledge Industry Co., LTD (MKI), and was funded by the New Energy and Industrial Technology Department Organization (NEDO) in Japan. The AGA2 has been created from bio-synthesized glycans, and contains only well-defined and characterized, biologically relevant, glycans1,2.

As well as adding new species to the database, the AGA2 system is now ready to use common fluorescence labeling schemes including 2-aminopyridine (2-AP / PA), 2-aminobenzoic acid (2-AA) and 2-aminobenzamide (2-AB) and allows for the use of any types of fluorescence labeling.

Acknowledgements
The AGA2 database is owned by the National Institute of Advanced Industrial Science and Technology (AIST) and is licensed to the Mitsui Knowledge Industry Co., LTD (MKI).

The search software is a product of MKI.

AGA2 uses the outcome of a study supported by the New Energy and Industrial Technology Department Organization (NEDO) in Japan.

References
1 Kameyama A, Kikuchi N, Nakaya S, Ito H, Sato T, Shikanai T, Takahashi Y, Takahashi K, Narimatsu H. Anal Chem. 2005 Aug 1;77(15):4719-25.
2 Kameyama A, Nakaya S, Ito H, Kikuchi N, Angata T, Nakamura M, Ishida HK, Narimatsu H. J Proteome Res. 2006 Apr;5(4):808-14.