The fastest gradient cycle time is achieved with the innovative hardware technology. The total system delay volume has been reduced to less than 42 µL with the ultra-low volume (20 µL) mixer, optional loop injection kit with 5 µL loop and micro-volume pre-heater.
Because complex samples require high-sensitivity analysis, the front-end component for LC/MS(/MS) must decrease its background noise and carryover to zero. The SIL-30AC autosampler enables excellent LC/MS(/MS) analysis with near-zero carryover and, when necessary, can utilize its multi-rinse functionality, incorporating up to 4 different rinse solutions, for an even cleaner analysis.
Nexera and LCMS-2020
Simultaneous Analysis with Shimadzu LCMS-2020
- Positive
-
- 1. Atenolol
- 2. Procaine
- 3. Lidocaine
- 4. Atropine
- 5. Yohimbine
- 6. Chlorpheniramine
- 7. Propranolol
- 8. Alprenolol
- 9. Tetracaine
- 10. Diphenhydramine
- 11. Doxepin
- 12. Dipyridamol
- 13. Desipramine
- 14. Imipramine
- 15. Nortriptyline
- 16. Amitriptyline
- 17. Dibucaine
- 18. Verapamil
- 19. Reserpine
- 20. Carbamazepine
- 21. Isopropylantipyrine
- 22. Alprazolam
- 23. Trizolam
- 24. Cilostazol
- 25. Nifedipine
- 26. Diazepam
- 27. Warfarin
- Negative
-
- 1. Cefuroxime
- 2. Chloramphenicol
- 3. Nitrendipine
- Column
- : ODS column (2.1mm i.d. x 50mm, 1.8µm)
- Mobile phase
- : A: 0.1% formic acid in water
B: 0.1% formic acid in acetonitrile - Gradient
- : B 3% → 95% (0.50 min)
- Flow rate
- : 1.8mL/min
- Column temp.
- : 50ºC
- Detection
- : ESI (+/-), LCMS-2020
- Scan speed
- : 15000 u/sec
Complete Reduction of Sample Carryover by Multi-Rinse Functionality
[Analytical Conditions]
- Column
- : ODS column (2.1 mm i.d. x 50 mm, 2.2 µm)
- Mobile phase
- : A: 0.1% formic acid
B: acetonitrile - Flow rate
- : 1.5 mL/min
- Gradient
- : B 25% → 50% (0.75 min) → 90% (0.76 min)
- Column temp.
- : 40ºC
- Detection
- : LCMS-2020, ESI (+)
- Rinse solution
- : R0: 0.1% formic acid in acetonitrile
: R1: 0.1% formic acid in methanol - Sample
- : desipramine, amytriptyline (each 5 µg/mL)
- Injection vol.
- : 5 µL
- ①: Needle Dip Rinse
- ③: Internal Needle Rinse
- ②: Needle Pump Rinse
- ④: Injection Port Rinse
| Desipramine | Amytriptyline | |
|---|---|---|
| ①+②+③+④ | Not Detected (<0.0042% = 1.0pg) | Not Detected (<0.0027% =0.68pg) |
| ①+②+③ | Not Detected (<0.0042% = 1.2pg) | Not Detected (<0.0027% =0.68pg) |
| ①+②* | Not Detected (<0.0042% = 1.2pg) | Not Detected (<0.0027% =0.68pg) |
| ①* | 0.01% | 0.01% |
| no rinse | 0.05% | 0.05% |
The SIL-30AC autosampler incorporates pre-treatment functionalities such as dilution, addition of an internal standard and pre-column derivatization schemes. These new features enable unattended operation for increased accuracy, precision and speed for high-throughput operation.
SIL-30AC Autosampler
Analysis of o-phthal aldehyde using SIL-30AC automatic pretreatment function.
- Column
- : ODS column (3.0mm i.d. x 50mm, 2.2µm)
- Mobile phase
- : A: 20 mmol/L Potassium Phosphate
B: Acetonitrile - Flow rate
- : 1.2 mL/min
- Column temp.
- : 40ºC
- Detection
- : Fluorescence at Ex 350 nm, Em 450 nm
Green chromatography requires stable and reliable temperature control at a high temperature. The CTO-30A column oven provides precise temperature control up to 150ºC with a newly designed solvent pre-heater, a proprietary Intelligent Heat Balancer (IHB) and post-column solvent cooling. The solvent pre-heater and IHB ensure uniform column temperature even at the high flow rates achievable with Nexera.
CTO-30A Column Oven
Comparison between Green and Conventional LC
[Green LC]
- Column
- : Polymer-based ODS (3 mm i.d. x 150 mm, 3 µm)
- Mobile phase
- : Water only
- Flow rate
- : 0.5 mL/min
- Column temp.
- : 150ºC
[Conventional LC]
- Column
- : ODS column (4.6 mm i.d. x 150 mm, 5 µm)
- Mobile phase
- : Methanol/Water (30/70)
- Flow rate
- : 1.0 mL/min
- Column temp.
- : 40ºC
