Shimadzu's Nexera is a genuine all-around HPLC that enables various types of analysis including conventional LC, ultra fast LC and ultra high-pressure LC up to 130 MPa without compromising performance and data quality. The maximized pressure, minimized delay volume and fast injection enable ultra-high speed cycle times in addition to maximizing peak capacity. With a newly-designed column oven, Nexera is useful for a wide variety of applications such as high-temperature analysis and green HPLC. Additionally, the pretreatment autosampler extends the application range for full automation and maximum performance.
The micro plunger-driven precision solvent delivery control can keep up with the demanding flow rate changes required during the rapid concentration shifts in fast gradient programming. Nexera also offers an ultra-low volume mixer based on micro reactor technology and a micro-volume pre-heater to minimize band broadening in high-temperature conditions. The precise solvent delivery control, high-efficiency mixer and micro volume pre-heater combine to ensure accurate and precise chromatography with excellent retention time reproducibility, resulting in secure detection of target compounds in complex matrices.
The SIL-30AC autosampler achieves excellent injection volume reproducibility for even 0.1 µL injections through use of a high-resolution metering system (6nL/step) and an atmospherically-sealed valve system. The autosampler meets reliability requirements in all analyses – from micro to conventional to UHPLC separations.
| Injection Vol. [µL] | Reproducibility (%RSD, n=6) |
|---|---|
| 0.2 | 0.281 |
| 0.4 | 0.237 |
| 1 | 0.111 |
| 2 | 0.039 |
| 5 | 0.031 |
| 10 | 0.043 |
| 20 | 0.044 |
Shimadzu continues to improve its low-carryover reputation with the Nexera autosampler. With reduction of the needle contact area, special coatings, surface treatments and a new needle seal, the SIL-30AC autosampler reaches a new level of low carryover performance.
In addition to minimizing the fundamental causes for compound adsorption, Nexera offers multiple rinse modes with multiple rinse solvents to eliminate carryover of even the most stubborn of compounds. Active rinsing of all contact surfaces can be selected.
Trace-level measurements, such as impurities analysis, require a wider dynamic range and lower baseline disturbances. With a temperature-controlled flow cell and a stray-light correction function, both the SPD-20A UV and SPD-M20A Photo Diode Array detectors offer the high level of stability, sensitivity and linearity required for confident analysis.
The 100 Hz sampling of the SPD-20A/M20A ensures peak information can be captured without loss of resolution and exploits the full potential of ultra-fast LC columns.
- Column
- : ODS (2.1mm i.d. ×100 mm, 1.8µm)
- Mobile phase
- : A: water
B: acetonitrile - Gradient
- : B 25% → 40% (0.1 min) → 70% (3.0 min)
- Flow rate
- : 0.8 mL/min
- Column temp.
- : 30ºC
- Detection
- : 240 nm
Sensitivity is the most important factor for fluorescence detection. With a newly designed optical system, RF-20A/20Axs offer world-leading levels of sensitivity. A water Raman S/N ratio of at least 2000 for RF-20Axs or 1200 for RF-20A makes these detectors powerful tools for analyses that demand the detection of trace-level components.
RF-20Axs features a temperature-controlled cell with a cooling function. It maintains a constant detector cell temperature, even if the room temperature fluctuates significantly, to ensure superb reproducibility with no drop in sensitivity.
The 100 Hz sampling of the RF-20A/20Axs permits ultra-fast LC analysis with no loss of resolution.
Quinolones chromatogram
- Column
- : ODS (3 mm i.d.×50 mm, 1.8 µm)
- Mobile phase
- : A: 0.05% formic acid
B: 0.1% formic acid in acetonitrile - Gradient
- : B 8% → 16%(0.75 min) → 36%(1-1.5 min) → 95%(2 min)
- Flow rate
- : 2.0 mL/min
- Column temp.
- : 55ºC
- Detection
- : Ex. 295 nm, Em. 455 nm
(0-1.05 min)
Ex. 325 nm, Em. 365 nm
(1.05-3 min)
