PPSQ-31A/33A Protein Sequencers
Confident Protein/Peptide Sequencing

Improved user friendliness by combining newly developed
software with reliable technology of proven PPSQ series.

Protein Sequencer


Protein sequencing via automated Edman degradation chemistry remains a simple and robust solution for many labs, delivering unambiguous data that is much less complex than the data obtained through mass spectrometry-based techniques. Utilizing state-of-the-art HPLC analysis, high sensitivity detection and powerful new acquisition/analysis software, Shimadzu's PPSQ series is no exception, achieving greater simplicity and reliability in the determination of amino acid sequences.


Excellent Baseline Stability

Using the highly acclaimed Shimadzu HPLC, the PPSQ series protein sequencers separate PTH-amino acids isocratically, improving baseline stability and allowing high-sensitivity analysis of PTH-amino acids.

Superior Retention Time Reproducibility

Isocratic sequence analysis provides more stable retention times. That means peaks detected in previous cycles can be cancelled using substation chromatogram processing, making it easier for users to identify sequences.

Reduced Liquid Waste/Running Costs

Analysis in isocratic mode allows for eluents to be recycled, thereby reducing the amount of mobile phase required along with reduced waste disposal, resulting in significant cost savings. It also allows for quick instrument startup as the HPLC can be left in a standby re-cycle mode.

Newly developed software

The PPSQ series' specialized software incorporates control functions for the reaction unit and HPLC, enabling easier sequence analysis of proteins and peptides. Simple, easy-to-use data analysis functions simplify the following:
  • Reprocessing of chromatograms
  • Overlay of multiple chromatograms
  • Automatic estimation of amino acid sequences

PTH-amino acid analysis in isocratic mode

Retention time stability, simple HPLC maintenance, and reduced running costs have been achieved by adhering to isocratic PTH-amino acid analysis with a mobile phase circulation system. Waste liquid is minimized because the mobile phase can be reused.


Main Unit PPSQ-31A/33A
Reaction method Edman degradation
Reaction time PPSQ-31A: 47 min/cycle, PPSQ-33A: 48.5 min/cycle
Number of reactors PPSQ-31A: 1, PPSQ-33A: 3
Sample immobilization method Glass fiber disk (8 mm dia.) or PVDF membrane
Reactor temperature control {Room temperature + 10 °C} to 60 °C
Converter temperature control {Room temperature + 10 °C} to 70 °C
Column temperature control {Room temperature + 10 °C} to 60 °C
Number of reagents/solvents 8 (one is an option)
Reagent/solvent delivery method Nitrogen gas pressure
Dimensions 650 (W) x 520 (H) x 500 (D) mm
Weight PPSQ-31A: 55 kg

For Research Use Only. Not for use in diagnostic procedures.