Preparative Chromatography Systems
Liquid Chromatography


Column Scales and lineup

Column Scales and lineup

Shimadzu has installed more than 2000 preparative HPLC systems throughout the world, and remains committed to improving the purity and speed of separation and making this technique more cost effective. Using our broad experience and technologies at various scales and in diverse fields, we have significantly enhanced the performance that can be obtained from preparative HPLC.

Wide Range of Use from Analysis to Large-Scale Fractionation: LC-20AP

LC-20AP Preparative System

LC-20AP Preparative System

High-speed fractionation can be performed using a column with a high separation capacity.
The new LC-20AP preparative pump has improved pressure performance under high flow rates (42 MPa for up to 100 mL/min, 30 MPa for up to 150 mL/min), enabling the use of a higher separation column, so fractionation with high purity can be achieved even for minute quantities of target compounds, such as impurities in pharmaceuticals. At the same time, the solvent delivery accuracy at low flow rates has been increased, so the system has a high basic performance over a broad range of flow rates from analytical to large-scale fractionation. Furthermore, its power can be used over a wide range of applications from fractionation of natural products to analysis of impurities.

In addition to high basic performance, the system is energy efficient, consuming less power and achieving an energy savings of about 41%*. Finally, the width has been reduced from 350 mm (previous model: LC-8A) to 260 mm, for a space savings of about 25 %.

* Comparison of measurement results for the LC-20AP and LC-8A for solvent delivery at 5 MPa pressure and 40 mL/min flow rate.

Large Scale Preparative System (Automated Scale-up System)

Large Scale Preparative System (20AP type)

Large Scale Preparative System (20AP type)

This fractionation system uses the LC-20AP solvent delivery unit. The maximum flow rate is a powerful 150 mL/min, and with a 20-50 mm internal diameter column, it is suitable for automatic continuous fractionation. With an analytical column (1 mL/min), studies of separation conditions or load quantities, or verification of the purity of the fractionated liquid, can be performed. The system can be configured from a variety of optional accessories, such as 5 sample injectors, 3 recycle valves, 2 fraction collectors, etc.

Low-Pressure Gradient Preparative System

Low-Pressure Gradient Preparative System
This preparative system uses the FCV-200AL, low pressure quaternary valve, coupled with the LC-20AP preparative pump covering the semi-preparative scale using a 2 cm to 3 cm I.D. column at flow rates of up to 50 mL/min. The system is suitable for gradient analysis in the semi-preparative scale that allows selection of up to four mobile phases per solvent module. The LC-20AP offers excellent basic performance for high-flow rate pumping and permits use of a high-resolution column or high-performance column. It provides a powerful tool for a wide range of applications from synthesis checks and purification of compounds to the fractionation of natural substances and impurity analysis.

High-Performance Preparative Recycle System

A preparative recycle system uses a solvent delivery unit that has the ability to create a closed loop recycle separation. When the system is combined with the appropriate column, it is possible to obtain a theoretical plate number greater than 1 million, making it possible to separate benzene and mono-deuteriated benzene substitute, which have only extremely small difference in properties (see figure below). Also, if the elution volume, until the appearance of the peak is greater than 35 mL (for semi-prep) or 100 mL (for prep), recycle separation can also be performed with an analytical size column. Recycle separation is perceived as a time-consuming technique, but in separation of neighboring components, the preparative processing speed per unit time can be increased using recycle separation in which a large amount of sample is injected.

Preparative Recycle System

The large scale preparative recycle system uses the LC-20AP solvent delivery unit with a maximum flow rate of up to 150 mL/min. The LC-20AP solvent delivery unit has a recycle flow line inlet built into the pump inlet to reduce system volume.
Preparative Recycle System
Semi-preparative Recycle System

The semi-preparative recycle system uses the LC-6AD solvent delivery unit with a recycle kit that minimizes the internal volume. It is mounted to the pump to enable high-efficiency recycle separation (closed loop recycle separation).
High-Performance Preparative Recycle System

Recycle Assist Software

Low-Pressure Gradient Preparative System
Special Prominence Preparative Recycling Software

Recycle Assist SW allows preparative recycling for Large Scale Preparative System or Semi-Preparative System with a user friendly interface. Recycle Assist SW provides a closed looped recycling environment in a preparative recycling HPLC system to improve separation capacity for chiral separation, anhydrous GPC analysis and other separation modes that require expensive solvents or very long preparative columns. Recycle Assist software interface shows the entire process, from recycling to fractionation, on one screen for ease of use and simplicity.

Bio-Chemical Analysis/ Preparative System (Inert LC System)

This system uses a PEEK polymer for liquid-contacting parts; PEEK is resistant to mobile phases containing halogenated ions and acids. The auto injector, column oven and fraction collector have a cooling function, providing efficient separation at low temperatures. High-sensitivity detectors with a PEEK polymer cell include the UV-VIS, photodiode array, and fluorescence detectors.