RF-5301PC Spectrofluorophotometer

From Routine Analysis To Research, Employing
The Highest Level of Sensitivity In The World!

RF-5301PC - Fluoresence
Compared to absorbance methods, fluorescence sensitivity is tens to thousands times better - this means that you can analyze nanogram to picogram samples with great results.

Fluorescence can be used also to identify a specific molecule in a complex background. When the compound of interest does not exhibit natural fluorescence, functional group-specific probes may be used to label the compound and assist your research. The synchronous scanning mode allows mixtures of fluorochromes to be analyzed.

The personal computer directly controls the instrument for data acquisition and processing. The Windows friendly operating environment allows you to perform measurement, data processing, editing and recording in one continuous operation with a click of the mouse. Using the Copy Graph function, measurement data or spectra may be easily transferred to word processing or spreadsheet software for preparation of documents or additional calculations.

The essence of fluorescence analysis is sensitivity. The high throughput optical system in the RF-5301PC employs a blazed holographic grating, photomultiplier and digital signal processing to provide the highest level S/N ratio attainable.

  • High Speed Scanning

  • High Resolution and Extended Range

  • Built-In Performance Checks

See The Difference With WindowsTM Performance

Working in the WindowsTM environment makes operation intuitive. The software is driven by pull-down menus displayed at the top of your screen, that can be selected with just a click of the mouse. For the beginner, on-line HELP is available to guide you through each function. You can customize the software with SpeedBox, an innovative tool that eliminates keystrokes and enables you to assign icons for commonly used menu commands. With the RF-5301PC software, storage, retrieval and other file management is quick and simple.

Hardware Specifications

Light Source 150W Xenon lamp. Ozone resolving type lamp housing.
Excitation and emission monochromators Concave, blazed holographic grating, F/2.5, 1300 grooves/mm.
Wavelength scale 220-900nm.
Measuring wavelength range 220-750nm and 0 order as standard. 220-900nm with the optional R928 photomultiplier.
Spectral bandwith 6-step selection of 1.5, 3, 5, 10, 15 and 20nm. (6nm bandwith is available for half sample height on the excitation side only.)
Wavelength accuracy ±1.5nm.
Light source compensation Dynode feedback system with monochromatic light monitoring.
Sensitivity The S/N ratio is 150 or higher for the Raman line of distilled water (350nm excitation wavelength, 5nm spectral bandwidth, and 2 second response for 98% of the full scale).
Wavelength scanning 7-step selection of Survey (about 5500nm/min), Super (about 3000nm/min), Very Fast, Fast, Medium, Slow and Very Slow.
Wavelength slewing speed About 20,000nm/min.
Response 8-step selection of 0.02, 0.03, 0.1, 0.25, 0.5, 2, 4, and 8 seconds for 98% of the full scale.
Sensitivity selection 2-steps of HIGH and LOW. (The sensitivity at HIGH is about 50 times that of LOW.)
Interface RS-232C interface, interface for autosampler, and interface for sipper unit.
Dimensions and weight 667W x 530D x 270H mm; 43 kg.
Power requirements 100, 120, 220, 240V; 50/60Hz; 400VA.
Operational temperature range 15-35°C
Operational humidity range 40-80% (Below 70% with temperature higher than 30°C).

Software Specifications

Measurement Excitation, emission and synchronous spectrum measurement, time-course measurement, quantitation, automatic search of optimal excitation and emission wavelengths, PopUp Scan.TM
Data processing Arithmetic calculation between spectra and between a spectrum and a constant, smoothing, 1st through 4th derivatives, 1/Y, logarithmic conversion, data printout (with or without activity value computation), peak pick, point pick, area calculation, averaging (in quantitation), generation of calibration curves of 1st through 3rd order.
Filing Save, recall, and delete, of data. Conversion into ASCII and DIF formats.
Data output Automatic scale adjustment, readout of data at user-specified point, data printout (preview function provided), selection of colors and types of curves.
Maintenance Automatic monitoring of signal-to-noise ratio, monitoring of the run time of light source lamp.
User interface SpeedBoxTM (assign icons for commonly used menu commands).
Other functions Data exchange via clipboard, auto response control, automatic shutter.
PC requirements IBM-PC/AT or 100% compatible; i486 or higher CPU; 8 Mbyte or larger main memory. Operates on MS-Windows version 3.1 or higher.