Separation of Lipids using the Nexera UC Supercritical Fluid Chromatograph

Lipids are often referred to as compounds derived from living organisms that dissolve in organic solvents, and there are many types including complex lipids that have polar groups such as phosphate groups and sugars in their structure, and simple lipids such as fatty acids and acylglycerol. They are classified into lipid classes with common functional groups in their structure, and the physical properties differ greatly depending on the class. There are also different molecular species of constituent fatty acids within the same lipid class. Therefore, a high separation capacity is required for lipid analysis, and with HPLC the normal phase mode and reversed phase mode are applied differently according to the purpose. Generally, the normal phase mode is used when separating into lipid classes and the reversed phase mode is used when separating molecular species. The mobile phases used in the two modes are completely different, and it is very troublesome to switch modes in an analysis. Here, we introduce separations by supercritical fluid chromatography (SFC) using various columns with different stationary phases.

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Application News
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High Performance Liquid Chromatography (HPLC & UHPLC)
sfc, supercritical, fluid, lipids, separation, Pharma & Biopharma, Small Molecule Analysis, Nexera UC
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