Peptide Mapping of Antibody Drugs by Nexera-i

Peptide mapping by HPLC is one of the important quality assurance tests used for verifying the primary structure of antibody drugs. Typically, following enzymatic digestion of the antibodies, separation is conducted using a traditional reversed phase column. Due to the large number of peaks that require separation, the use of small-particle columns and core shell columns for peptide analysis has spread in recent years. In order to compare elution profiles for identity and mutation confirmation, a highly repeatable system is required. The Nexera-i integrated UHPLC is the ideal system for such an analysis. Here, the Nexera-i is used in the analysis of IgG (human immunoglobulin G) tryptic digest.

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Application News
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High Performance Liquid Chromatography (HPLC & UHPLC)
peptide, mapping, antibody, drugs, Pharma & Biopharma, Monoclonal Antibodies and Biosimilars, Nexera-i, i-series
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For Research Use Only. Not for use in diagnostic procedures.

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