Nexera Analytical UHPLC/SFC Method Development Solutions
Efficient Method Development using the UHPLC/SFC Switching System
When considering separation conditions, using both UHPLC and SFC can help to optimize conditions further. The Nexera UC/s UHPLC/SFC switching system provides the ability to use both UHPLC and SFC analysis modes in a single system. The figure below shows a flow diagram for this system. The system was configured by adding a supercritical carbon dioxide delivery unit and back pressure regulator unit to a standard UHPLC system. Both UHPLC and SFC analysis modes can be used by switching of delivery units (control mode ON or OFF) and switching the pressure of the back pressure regulator.
Sharing the solvent delivery unit (for pumping organic solvents), autosampler, column oven, and detector for both SFC and UHPLC analysis minimizes space requirements and equipment cost and reduces equipment downtime. In addition, an existing UHPLC system can be upgraded to this system.
By using the mobile phase solvent switching valve in combination with the column switching valve, mobile phase conditions can be changed automatically and continuously for up to twelve columns to enable a wide variety of conditions, improving method development efficiency.
The following describes an example of using the UHPLC/SFC switching system to increase the speed of method scouting. The Nexera UC/s UHPLC/SFC switching system was used to automatically optimize the separation conditions for two chiral compounds (omeprazole and warfarin).
A total of 36 combinations of six chiral columns (CHIRALPAK® series) and the three mobile phases shown in the tables below (18 combinations each for UHPLC and SFC) were evaluated.
Reproducibility for continuous switching
Using the switching system, three drug components were analyzed by continuous switching between UHPLC and SFC analysis three times. The resulting chromatograms are shown in Fig. 10. The chromatograms show that reliable results were obtained, with no effects from switching flow lines, even if mobile phases and separation characteristics are significantly different.