Absorbance measurement is used in various fields as a means to quantitate substances. However, when the sample for measurement has a low concentration, measurement requires higher sensitivity and therefore fluorescence
measurement may be employed instead. Particularly in the fields of chemistry and life science, there are cases where the amount of sample that can be obtained is insufficient for measurement, calling for techniques that enable measurement of even smaller trace amounts of sample. This article introduces trace measurement results of chlorophyll-a using TrayCellTM (manufactured by Hellma Analytics), which enables fluorescence measurement of samples of only a few μL, and a sub-micro cell together with the RF-6000 spectrofluorophotometer.