Micro Volume Photometric Measurement of Nucleic Acid using TrayCell with UV-1280

Nucleic acid concentration can be determined easily using an ultraviolet-visible (UV-VIS) spectrophotometer. Such samples are typically available in small micro volume (less than 5 μl), and a system that is able to measure micro volume with high reproducibility will be preferred. A conventional 10 mm path length super micro cuvette requires at least 50 μl of sample and needs extra care in cleaning to prevent carryover. Hellma TrayCell is a fiber-optic ultra-micro cell designed for the UV-VIS micro volume analysis of nucleic acids and proteins. The sample volume requirement ranges from 0.7 μl to 10 μl depending on the path length used. Shimadzu UV-1280 offers a bio method mode as a standard function for DNA and protein concentrations determination.

Content Type:
Application News
Document Number:
AD-0088
Product Type:
Keywords:
micro volume, photometric, nucleic acid, tray cell, Life Science Solutions, uv-1280
File Name:
Ad0088spectro.pdf
File Size:
266kb

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For Research Use Only. Not for use in diagnostic procedures.

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