Detection of hormones (E1, E2, EE2) according to the requirements of the EU Water Framework Directive using an online-SPE-HPLC-MS/MS

The watch list was established by the European Union as part of the EU Water Framework Directive. It includes the estrogen active substances estrone (E1), 17β-estradiol (E2) and 17α-ethinylestradiol (EE2). Necessary detection limits (LODs) are 400 pg/L for E1 as well as E2 and 35 pg/L for EE2. A recent status report by the EU showed the progress, especially in the detection of EE2. So far, no laboratories have been able to achieve the requirements for EE2. The challenge for a robust method for quantification of estrogens in the ultra-trace range in water is due to the separation of interfering matrix compounds. In our study, we developed a highly selective sample preparation in combination with online-SPE-HPLC-MS/MS.

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Document Number:
ASMS 2020 - ThP 083
Product Type:
Liquid Chromatography Mass Spectrometry, Gas Chromatography Mass Spectrometry
Environmental, LCMS-8060 Triple Quadrupole Liquid Chromatograph Mass Spectrometer (LC-MS/MS), GCMS-TQ8040 Triple Quadrupole GC-MS
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