Fully automated LC-MS/MS method to assess DPD deficiency in Cancer treatment with 5-FU

Fluoropyrimidines (5-fluorouracil or capecitabine) are anticancer drugs used in nearly 60% of chemotherapy treatment. It is known that they can lead to severe or lethal toxicities in case of dihydropyrimidine dehydrogenase (DPD) deficiency, therefore it is highly recommended to check for that DPD efficiency status. In France, health authorities recommend the determination of uracil concentration to guide dosing of fluoropyrimidines. Numerous LC-MS/MS methods have been proposed but they include complex liquid-liquid or solid-phase extraction procedures. To answer to the need of high throughput and robust analysis, our objective was to develop a method where the extraction was carried out by a programable liquid handler robot directly coupled to a LCMS/ MS system.

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Document Number:
ASMS 2020 - TP 120
Product Type:
Liquid Chromatography Mass Spectrometry, Life Science
Clinical Research, LCMS-8060 Triple Quadrupole Liquid Chromatograph Mass Spectrometer (LC-MS/MS), CLAM-2030 Fully Automated Sample Preparation Module for LCMS
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