Method development of high-throughput eicosanoid profiling for micro-sampling plasma

Eicosanoids or omega-3 fatty acids metabolites are attractive targets for understanding pathophysiological functions and discovering disease biomarker. We reported that we developed multiple reaction monitoring (MRM) method to detect fatty acid metabolites quantitatively in disease model mice serum in ASMS2019. To make it the faster and the more sensitive, the number of MRMs was narrowed down for the detected targets and internal standards. Consequently, we have accomplished 10 minutes’ LC-MS/MS method consisted of 200 MRMs for monitoring target 114 eicosanoids and related fatty acid metabolites. The method was applied to 5.6 μL of healthy human serum and plasma corrected by microsampling devise (MSW2) to detect major arachidonic acid metabolites.

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ASMS 2020 - ThP 362
Product Type:
Liquid Chromatography Mass Spectrometry
Life Science Solutions, LCMS-8060 Triple Quadrupole Liquid Chromatograph Mass Spectrometer (LC-MS/MS)
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