Human embryonic stem cells (hESCs) are promising tools for diseasemodeling,
cell therapy, bio-industry or drug development. However, longterm
cultured hESCs finally develop hidden phenotypic changes,
cumulatively acquire various alterations on both the genetic and non-genetic
levels and despite advanced culture techniques, the culture-adapted clones
with unwanted properties clones are inevitably selected. However, these
changes could remain unnoticed until they alter the genome, karyotype or
cell phenotype, even in case of the high expression of stemness-associated
transcription factors, or their differentiation capacity, or a typical morphology.
Furthermore, molecular, genetic, and/or light-microscopy analyses can fail
in the case of the genetically or karyotypically silent changes that are
evoked in cultured cells. Thus, recent quality control approaches often suffer
of low sensitivity or may produce biased output. Therefore, there is an
ongoing need for sensitive, robust, feasible and affordable methods
revealing abnormalities in cell phenotype.
- Content Type:
- Document Number:
- ASMS 2020 - TP 122
- Product Type:
- Life Science, MALDI-TOF Mass Spectrometry
- Life Science Solutions, MALDI-8020 Benchtop Linear MALDI-TOF Mass Spectrometer
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