Monitoring of embryonic stem cell differentiation trajectories by intact cell mass spectrometry

Human embryonic stem cells (hESCs) are promising tools for diseasemodeling, cell therapy, bio-industry or drug development. However, longterm cultured hESCs finally develop hidden phenotypic changes, cumulatively acquire various alterations on both the genetic and non-genetic levels and despite advanced culture techniques, the culture-adapted clones with unwanted properties clones are inevitably selected. However, these changes could remain unnoticed until they alter the genome, karyotype or cell phenotype, even in case of the high expression of stemness-associated transcription factors, or their differentiation capacity, or a typical morphology. Furthermore, molecular, genetic, and/or light-microscopy analyses can fail in the case of the genetically or karyotypically silent changes that are evoked in cultured cells. Thus, recent quality control approaches often suffer of low sensitivity or may produce biased output. Therefore, there is an ongoing need for sensitive, robust, feasible and affordable methods revealing abnormalities in cell phenotype.

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Document Number:
ASMS 2020 - TP 122
Product Type:
Life Science, MALDI-TOF Mass Spectrometry
Life Science Solutions, MALDI-8020 Benchtop Linear MALDI-TOF Mass Spectrometer
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