Selective and sensitive quantification of glucagon in human plasma using microflow LC/Q-TOF MS

In this study, we developed both a simplified protocol of sample pretreatment and a sensitive quantitation method for plasma glucagon using a microflow LC and a high resolution accurate mass spectrometer with microflow ESI. Impaired secretion of endogenous bioactive peptides such as peptide hormones and cytokines is associated with the development and pathophysiology of various diseases. Glucagon is a peptide hormone associated with diabetes and known to increase blood glucose levels. There are several peptide hormones generated from the same precursor protein as glucagon. These glucagon-related peptides hamper specific detection of glucagon by a conventional immune assay due to its resembled structure. To selectively quantify glucagon in human plasma, we developed a sensitive method using a microflow LC/Q-TOF.

Content Type:
Poster
Document Number:
ASMS 2020 - TP 473
Product Type:
Liquid Chromatography Mass Spectrometry
Keywords:
Life Science Solutions, Nexera Mikros Microflow Liquid Chromatograph, LCMS-9030 Q-TOF Mass Spectrometer
Language:
English
File Name:
TP473-ASMS-2020-Poster.pdf
File Size:
1,035kb

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