Multi-target screening of toxicological compounds in blood on a fully-automated platform consisting of sample preparation module CLAM and LC-MS/MS

Multi-target screening by LC/MS/MS has been widely adopted in detection and quantitation of drugs of abuse (DoA) in forensic investigation and toxicological research [1]. Usually, a wide range of targets are screened in such analysis, including illicit drugs, narcotics, psychotropics, antipsychotics, pharmaceuticals and other toxic compounds in urine, serum/plasma and whole blood samples. Sample preparation is often a bottleneck due to the tedious steps. It is also a factor responsible for inaccurate or false negative results. We describe a solution by using an automated sample preparation module CLAM-2000 TM connected with LC/MS/MS system (LCMS-8060) for multi-target screening of 61 drugs in whole blood. A ready-to-use method package Rapid Toxicology Screening (Shimadzu) [2] was used to set up the screening method with human whole blood (frozen) spiked sample without efforts in LC and MRM method development.