RF-6000 Spectrofluorophotometer

The ultimate performance for a diverse range of customers' measurement needs

High-Sensitivity Measurements

■ High-sensitivity measurements can be performed with S/N ratios of 1,000:1 (RMS) or 350:1 (P-P).
■ Improved lower limit of quantitation. Measures concentrations up to 1 x 10-13 mol/L (fluorescein).

A redesigned optical system and signal processing system achieve the highest S/N ratio levels in its class.
Even extremely dilute samples can be measured accurately.

The RF-6000 can measure fluorescence spectra from fluorescein concentrations as low as 1 x 10-13 mol/L. Furthermore, due to an auto-gain control function that ensures measurements are performed using optimal measurement parameters, the system can perform accurate and highly quantitative measurements over a wide six-order dynamic range, from 10-13 to 10-7 mol/L.

Fluorescence spectra of Fluorescein and calibration curve

High-Speed Measurement

Enables ultrafast scanning at speeds up to 60,000 nm/min. All wavelength regions can be measured in only one second. 3D fluorescence spectra can also be measured quickly.

Stable Measurements
xenon lamp

The Xenon lamp offers long service life and high stability. The 2000-hour service life significantly reduces running costs. In addition, auto alignment technology allows customers to easily replace the lamp without tedious adjustment routines.

Long Wavelength Measurements

A low-noise photomultiplier is included standard. It offers high measurement sensitivity for long wavelengths up to 900 nm. Consequently, it can measure substances that exhibit fluorescence at longer wavelengths, such as chlorophyll and indocyanin green (ICG). Below are Excitation and Emission spectra of indocyanin green, which is used for testing hepatic function and hepatic spare ability. The fluorescence peak was at 810 nm.

Fluorescence Spectrum of Thylakoid membrene solution Cooled by Liquid Nitrogen

Fluorescence Spectrum of Thylakoid membrene solution Cooled by Liquid Nitrogen

  1. Measured with the help of professor Jian-Ren Shen of Okayama University.
  2. Measured using a low-temperature measurement unit. Contact Shimadzu for further details about the low-temperature measurement unit.

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