Shimadzu Nexera Series UHPLC - An Overview
A Trusted Legacy
How did we get here?
The new Nexera series builds on Shimadzu’s legacy of ruggedness, reliability and proven performance in HPLC/UHPLC since 1978. Shimadzu has earned the reputation as the most trusted name in LC.
Most Trusted Name in LC
Shimadzu liquid chromatographs are the most requested front ends for LCMS systems around the world. Shimadzu front ends allow you to get the most from your mass spectrometer, all day, every day.
- Repeatable Results
- Accurate Flows
- Accurate Gradients
- Low Pulsation
- Low Carryover
Shimadzu pumps are known for their:
- Ultra-low baseline pulsation
- High flow rate accuracy and gradient repeatability
- Consistent peak retention times all day, every day
Shimadzu Autosamplers are known for their:
- Ultra-low carryover
- High-speed sampling
- Large sample capacity with open-access ACMP
Shimadzu Detectors are known for their:
- Wide Linear Dynamic Range
- Stability (Repeatable Results)
- Low Noise (High Signal to Noise for high sensitivity analyses)
For difficult to separate analytes, LabSolutions software boasts the i-PDeA –peak deconvolution based on spectral differences.
New Benchmarks in HPLC
Mobile Phase Monitor – Intelligence Maximizing Reliability
- Stop the batch
- Replenish the mobile phase
- Purge-prime mobile phase lines
- Test the chromatography
- Re-run wasted samples
The new Nexera series monitors solvents gravimetrically and warns you if you don’t have enough mobile phase to complete the batch.
Auto-Diagnostic and Recovery
The name of the game in HPLC is repeatability. Flow anomalies can interfere with the chromatography and stand in the way of repeatable results. Air bubbles can create such flow anomalies.
- Evaluate the results
- Stop the batch
- Apply a corrective pump purge
- Re-equilibrate the system
- Restart the batch
Nexera looks for evidence of flow anomalies. If indicated, it pauses the batch, applies a corrective purge, then restarts the batch –all automatically.
Auto-diagnostic/Auto-recovery = Maximum Up-Time
Let's do a deep dive
So what is going on here?
This is an example of Analytical Intelligence. Nexera is constantly in a process of self-diagnosis. If it detects the presence of air bubbles, it pauses the current batch in LabSolutions. Next, it automatically starts a line purge on all solvent lines as a corrective action. It attempts to re-equilibrate and then restart the batch. Again, if air is indicated, the batch pauses, and so on…
Self diagnosis followed by corrective action
One-Click Standby Mode
The new Nexera systems offer:
- One click Standby mode - Energy consumption reduced by 81%
- Intelligent system start-up to optimize routine analysis
HPLC columns can be damaged by sudden pump starts and stops as well as extreme gradient changes, especially with polymeric packings.
The Nexera series automatically uses Smart Flow Control, ramping the flow rate gradually to the set point according to the status of the column oven.
A New Benchmark of Efficiency
Efficency Equals Productivity
Waiting for the autosampler to cool to sub-ambient temps wastes time. Nexera systems cool samples quickly, going from ambient to 5 ˚C in 20 min (10 min faster than other instruments).
We all have to add more samples for analysis during the day. Each time, the system has to recover its set temp, a big waste of time. Nexera systems do this 10 min faster than other instruments.
The true benefit of fast UHPLC separations cannot be realized if you have to wait for the autosampler to prepare the next sample injection.
9 seconds of savings
Shimadzu continues to lead the industry in efficiency and performance with the new Nexera. Injection to injection cycle times are 9 sec, six seconds faster than other systems for time savings all day long.
Traditionally, analysts had to carry out tedious manual derivatization steps themselves.
Example: Pre-column derivatization of amino acids.
Nexera’s exclusive Co-Injection automation can be used to perform derivatization within the sample loop before injection.
Mismatch between the sample matrix and mobile phase conditions can lead to poor peak shapes and critical loss of resolution.
Nexera offers the innovative, Co-Injection. This easy-to-program automation yields excellent peak shapes by injecting a weaker solvent along with the sample.
Dual Injection System
Imagine doing twice the amount of work in the same amount of time, effortlessly. Nexera can do two sample injections at the same time into independent flow channels. This is multiplexing made easy. Exclusive to Shimadzu, results can be summarized in a single report.
Efficency Saves Time
Instrument and Operational Qualification can be tedious and time consuming.
The new Nexera Systems offer automatic IQOQ operation including double digital signature security level and electronic delivery of IQOQ reports
A New Benchmark of Design
Design Impacts Space Allocation
Bench space is priceless. A thoughtful design pays attention to lab footprint. The new Nexera Systems are the smallest in the industry.
Design Impacts Productivity
Reduced footprint is only one side of the coin. What about sample capacity?
A plate changer expands the autosampler capacity.
- 7 racks of vials or 14 MTPs per plate changer.
- Up to 3 plate changers can be connected.
- Total sample capacity = 44 MTPs or 16,896 samples.
A Truly Useful Column Management Device
Design Impacts Data Integrity
In the spirit of true Data Integrity, the new column management devices (CMD) catalog a history of column performance.
Along with number of injections, critical performance parameters can be monitored to chart column health.
Make informed decisions and generate reports of column performance history.
An Intuitive and Interactive Touch Screen Controller
Design Impacts User Experience
Elegant design means thoughtful attention to the user’s experience. Nexera’s System Controller is based on the popular i-Series LCD touch screen for intuitive touch-control over system parameters.