Shimadzu Nexera Series UHPLC - An Overview

A Trusted Legacy

How did we get here?

The new Nexera series builds on Shimadzu’s legacy of ruggedness, reliability and proven performance in HPLC/UHPLC since 1978. Shimadzu has earned the reputation as the most trusted name in LC.

A trusted legacy of HPLC products

Most Trusted Name in LC

Shimadzu liquid chromatographs are the most requested front ends for LCMS systems around the world. Shimadzu front ends allow you to get the most from your mass spectrometer, all day, every day.

HPLC with LCMS-8060NX

- Dependable

- Repeatable Results

- Accurate Flows

- Accurate Gradients

- Low Pulsation

- Low Carryover


Nexera HPLC/UHPLC Pump - LC-40D X3

Shimadzu pumps are known for their:

  • Ultra-low baseline pulsation
  • High flow rate accuracy and gradient repeatability
  • Consistent peak retention times all day, every day
 Nexera HPLC/UHPLC Autosampler - SIL-40CX3

Nexera HPLC/UHPLC Autosampler - SIL-40CX3

Shimadzu Autosamplers are known for their:

  • Ultra-low carryover
  • High-speed sampling
  • Large sample capacity with open-access ACMP
 Nexera HPLC/UHPLC Photodiode Array Detector - SPD-M40

Nexera HPLC/UHPLC Detector - SPD-M40

Shimadzu Detectors are known for their:

  • Wide Linear Dynamic Range
  • Stability (Repeatable Results)
  • Low Noise (High Signal to Noise for high sensitivity analyses)

For difficult to separate analytes, LabSolutions software boasts the i-PDeA –peak deconvolution based on spectral differences.


Analytical Intelligence

New Benchmarks in HPLC

Mobile Phase Monitor – Intelligence Maximizing Reliability

Let's talk HPLC. We have all dne it - start a batch of runs but forget to check the mobilep phase! We have to...
  1. Stop the batch
  2. Replenish the mobile phase
  3. Purge-prime mobile phase lines
  4. Test the chromatography
  5. Re-run wasted samples

The new Nexera series monitors solvents gravimetrically and warns you if you don’t have enough mobile phase to complete the batch.


Auto-Diagnostic and Recovery

The name of the game in HPLC is repeatability. Flow anomalies can interfere with the chromatography and stand in the way of repeatable results. Air bubbles can create such flow anomalies.


  1. Evaluate the results
  2. Stop the batch
  3. Apply a corrective pump purge
  4. Re-equilibrate the system
  5. Restart the batch

Nexera looks for evidence of flow anomalies. If indicated, it pauses the batch, applies a corrective purge, then restarts the batch –all automatically.

nexera can look for evidence of flow anomalies

Auto-diagnostic/Auto-recovery = Maximum Up-Time


Let's do a deep dive

intelligent self-diagnostics and recovery

So what is going on here?

This is an example of Analytical Intelligence. Nexera is constantly in a process of self-diagnosis. If it detects the presence of air bubbles, it pauses the current batch in LabSolutions. Next, it automatically starts a line purge on all solvent lines as a corrective action. It attempts to re-equilibrate and then restart the batch. Again, if air is indicated, the batch pauses, and so on…

Self diagnosis followed by corrective action

One-Click Standby Mode


The new Nexera systems offer:

  • One click Standby mode - Energy consumption reduced by 81%
  • Intelligent system start-up to optimize routine analysis
one click standby mode - system sustainability test

With one click, you can get your UHPLC ready to run samples while you go about other business.

Smart-Flow Control


HPLC columns can be damaged by sudden pump starts and stops as well as extreme gradient changes, especially with polymeric packings.

smart flow control

The Nexera series automatically uses Smart Flow Control, ramping the flow rate gradually to the set point according to the status of the column oven.

A New Benchmark of Efficiency

Efficency Equals Productivity

minimizing temperature change

Waiting for the autosampler to cool to sub-ambient temps wastes time. Nexera systems cool samples quickly, going from ambient to 5 ˚C in 20 min (10 min faster than other instruments).


nexera time savings

We all have to add more samples for analysis during the day. Each time, the system has to recover its set temp, a big waste of time. Nexera systems do this 10 min faster than other instruments.

hplc racks

True Benefit

The true benefit of fast UHPLC separations cannot be realized if you have to wait for the autosampler to prepare the next sample injection.

9 seconds of savings

9 seconds of savings

Shimadzu continues to lead the industry in efficiency and performance with the new Nexera. Injection to injection cycle times are 9 sec, six seconds faster than other systems for time savings all day long.



Nexera’s exclusive Co-Injection automation can be used to perform derivatization

Traditionally, analysts had to carry out tedious manual derivatization steps themselves.

Example:   Pre-column derivatization of amino acids.

Nexera’s exclusive Co-Injection automation can be used to perform derivatization within the sample loop before injection.

Nexera Co-Injection Automation

nexera co-injection

Mismatch between the sample matrix and mobile phase conditions can lead to poor peak shapes and critical loss of resolution.

Nexera offers the innovative, Co-Injection. This easy-to-program automation yields excellent peak shapes by injecting a weaker solvent along with the sample.

Co-Injection mismatch

Dual Injection System


Imagine doing twice the amount of work in the same amount of time, effortlessly. Nexera can do two sample injections at the same time into independent flow channels. This is multiplexing made easy. Exclusive to Shimadzu, results can be summarized in a single report.

dual injection system animation

Efficency Saves Time

Auto IQ-OQ Maximizes Up-Time

Instrument and Operational Qualification can be tedious and time consuming.

The new Nexera Systems offer automatic IQOQ operation including double digital signature security level and electronic delivery of IQOQ reports

A New Benchmark of Design

Design Impacts Space Allocation

Bench space is priceless. A thoughtful design pays attention to lab footprint.  The new Nexera Systems are the smallest in the industry.

nexera - design - small space allocation

Design Impacts Productivity

Reduced footprint is only one side of the coin. What about sample capacity?

Nexera Rack Changer

A plate changer expands the autosampler capacity.

  • 7 racks of vials or 14 MTPs per plate changer.
  • Up to 3 plate changers can be connected.
  • Total sample capacity = 44 MTPs or 16,896 samples.

A Truly Useful Column Management Device

Design Impacts Data Integrity

design impacts data integrity

In the spirit of true Data Integrity, the new column management devices (CMD) catalog a history of column performance.

Along with number of injections, critical performance parameters can be monitored to chart column health.

Make informed decisions and generate reports of column performance history.

An Intuitive and Interactive Touch Screen Controller


Design Impacts User Experience

Elegant design means thoughtful attention to the user’s experience. Nexera’s System Controller is based on the popular i-Series LCD touch screen for intuitive touch-control over system parameters.

Nexera SCL-40 Screen


E-Book - Nexera UHPLC: Bringing the Power of AI to Analytical Laboratories

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