High-Resolution and High-Speed Simultaneous Analysis of Regulated UV-Adsorbents in Cosmetics using SPP Column

Many cosmetics contain ultraviolet light absorbents (UV- absorbents) to protect the skin from ultraviolet light. In Japan, the standards for cosmetics based on the Pharmaceutical Affairs Law (Ministry of Health and Welfare Notice No. 331, 2000) regulate the types and allowable quantities of UV-absorbents that may be used in cosmetic products. Since regulations can vary significantly between different countries and regions, quantification with HPLC is used to determine compliance with import/export regulations. This report presents an example of high-throughput analysis of 23 kinds of UV-absorbents using the Nexera™ LC system and Shim-pack Velox™ C18 SPP (superficially porous particles, core-shell) column. This is a larger number of compounds than covered in a previous report as it also includes some residues regulated by EU agencies.