HPLC Vials

Vial selection for an HPLC system is an important but often overlooked step. Several characteristics of the vials and septa are critical for optimum performance in an HPLC run. Prominence vials have been thoroughly tested for compatibility with all Shimadzu autosamplers, so superior performance is assured.


Glass Thickness

Uniformity of the vial bottom thickness ensures consistent needle depth from run to run, and minimizes user intervention for changing the needle depth setting in the hardware. Consistency of the vial thickness from batch to batch is one of 14 quality points strictly controlled during manufacturing.

Septa Quality

The 1.5 and 4 mL sample vials use a PTFE/silicone septum, and its cleanliness is suitable for both everyday applications and the most discriminating LCMS analyses. Bonding agents often contribute to sample contamination inside vials; because the septum is press-fit inside the cap, this potential issue is eliminated. In addition, it prevents the needle from pushing the septum into the vial.

Superior Quality Glass

Clear glass vials are made from Type I Class A borosilicate (33 expansion) which is the most chemically inert. Amber vials are made from Type I Class B (51 expansion). Major and trace constituents as determined by ASTM E438-92 are indicated below. For additional surface deactivation, we offer a silanized version of the 1.5 mL vials.
Major Constituents Type I Class A (33), weight % Type I Class B (51), weight %
SiO2 81% 73%
B2O3 13% 10%
Al2O3 2% 7%
BaO 0 – 2%
CaO 1%
Na2O 4% 6%
K2O 1%

Low Adsorption Glass

Prominence glass vials are manufactured with unique Low Adsorption and Extra Low Adsorption properties. Low Adsorption vials exhibit up to 5 times lower adsorption of polar compounds versus comparable products on the market.
A comparison of polar compound loss due to adsorption to the surface of the conventional vials (yellow curve) vs. Prominence Low Adsorption vials (blue and red curves for clear and amber vials) is shown in the graph. Sample concentration is in the micromolar range.
adsorption graph.bmp
With Extra Low Adsorption vials you can achieve up to 10 times lower adsorption than that of Low Adsorption vials or up to 50 times lower than that of some comparable products on the market. These unique characteristics are achieved without the use of silanization or any other surface treatment that may contaminate the vial and interfere with analysis.

Vial Mouth Opening

The wide 6mm vial opening provides a better target for autosampler needles and makes pipette filling easier and cleaner.

One-Twist Cap

For high throughput labs, our single-twist cap allows fast and easy sample preparation. For extra convenience, a 1.5 mL snap-cap vial is also available.

For Research Use Only. Not for use in diagnostic procedures.