Nexera X2 UHPLC System

Nexera X2 UHPLC System
The Shimadzu Nexera X2 ultra high-performance liquid chromatograph (UHPLC) is the most advanced chromatographic system available today. This UHPLC system offers unsurpassed chromatographic efficiency, sensitivity, resolution, stability, and reliability. The Nexera X2 is a truly flexible design that expands to tackle a broad range of UHPLC applications.

Nexera X2 covers a broad range of UHPLC application demands.

  • Precision engineered UHPLC pumps for pulse-free solvent delivery at up to 130 Mpa

  • Automated solvent preparation – solvent selection, solvent blending, and gradient elution in one system

  • Enhanced autosampler rinsing functions – up to 4 different rinsing solutions

  • Large sample capacity – can hold up to 324 - 1.5 mL vials at a time and expandable with the rack changer

  • Column oven with Intelligent Heat distribution

Click the name of a component to view more information. Nexera X2 Components Degasser Pump Autosampler System Controller Detector Column Oven
Precision Engineered UHPLC Pump

The Nexera X2 solvent delivery unit (LC-30AD) is a world class pump for providing the utmost efficiency and performance using micro-flow rates up to 10 mL/min. Capable of delivering pulse-free solvent with pressures up to 130 MPa for ultra-fast and ultra-high resolution separation.

Solvent Delivery Diagram
Automated Solvent Preparation

Up to 16 mobile phase combinations can be selected for a variety of uses. Automated solvent selection, solvent blending, and/or low pressure gradient elution.

UHPLC/HPLC Switching to Handle Both Analyses

Ability to alternate between UHPLC and HPLC systems using a flow control switching valve

HPLC Switching Diagram

Multi-Rinse Autosampler Functions

The Nexera X2 series autosamplers have improved its hardware structure to prevent adsorption of those sticky compounds and minimize carryover between injections. The SIL-30AC and SIL-30ACMP offer a multi-rinse mechanism for additional rinsing of the needle and injection port with up to 4 different rinsing solutions.

Multi-Rinse Autosampler Diagram

SIL-30ACMP Autosampler
Maximized Autosampler Throughput:

The SIL-30ACMP autosampler maximizes sample throughput allowing six microplates or 1.5 mL vial racks. This autosampler also uses an open access mechanism enhancing work efficiency by allowing users to place new samples into racks that are not involved in sample injection during analysis. And different types of rack plates can be used across the three sample racks.

Ultimate Sensitivity and Resolution

The Nexera X2 photodiode array detector (SPD-M30A) introduces a capillary type cell which gives higher sensitivity and lower dispersion for superior data.

Sensitivity Comparison

LabSolutions Method Scouting for Method Development System

This system streamlines the process for more efficient and accurate method development by automatically switching between up to 96 combinations of unique separation conditions (with up to 6 columns, 8 mobile phases, and 10 gradient settings).

method scouting diagram

CBM web browsing software screen
CBM Web Browsing

Ability to monitor and control you LC system over a network.

Intelligent Heat Balancing Column Temperature Control with Solvent Pre-heater

The intelligent heat balancing mechanism and solvent preheater allows for uniform temperature control throughout the column.

Hardware Specifications (Limited)

Here are limited specifications for the typical components used in the Nexera XR system.

Detailed specifications are located within the component's pages.

System Controllers
Column Ovens
Degassing Unit 3 or 5 Lines (Volume 400 μL)
Flow rate range 0.0001 to 10 mL/min
Flow rate precision No more than 0.06% RSD or 0.02 min SD, whichever is greater
Gradient type High-pressure / low-pressure mixing gradient
Gradient / concentration precision ±0.5% (specified conditions)
Maximum pressure 130 MPa (0.0001 to 3 mL/min)
80 MPa (3.0001 to 5 mL/min)
22Mpa (5.0001 to 10 mL/min)
Injection method Needle-in-flowpath Needle-in-flowpath
Injection volume accuracy ±1% (at 50 μL injectiong, n = 10) ±1% (at 50 μL injectiong, n = 10)
Injection volume range 0.1 to 50 μL (Option: 0.1 to 100 μL) 0.1 to 50 μL (Option: 0.1 to 100 μL)
Injection volume reproducibility RSD: 0.3% maximum (at 10 μL) RSD: 0.2% maximum (at 10 μL)
Samples capacity Sample plate: 175 (1 mL), 105 (1.5 mL), 50 (4 mL), 2 (MTP/DWP plates)
Control plate: 10 (1.5 mL)
Sample plate: 324 (1.5 mL), 576 (96 well MTP/DWP Plates), 2304 (384 well MTP/DWP plates)
Control plate: 10 (1.5 mL)
Sample cooler Block cooling/heating, used together with defumidifying function
4 to 40°C
Block cooling/heating, used together with defumidifying function
4 to 40°C
Oven Capacity Up to 2 - 150 mm columns
Temperature control range Room temperature ± 5 to 150°C, Setting range 4 to 150°C
PDA Detector
Wavelength Range 190 to 800 nm
Noise level ±3 × 10−6 AU, (250 nm, Specified condition)
Flow cell 10 μL (10 mm), 8 MPa
Option cell High-Sensitivity: 8 μL (10 mm)

Easy to use and intuitive data acquisition and analysis software to control your LC or GC systems

Several software packages to find the one that fits your needs; LabSolutions, LabSolutions CS, LabSolutions DB, etc.

Fully compliant with a variety of regulations and guidelines including:
  • CSV, Ministry of Health
  • Labor and Welfare ER/ES guidelines
  • FDA 21 CFR Part 11

LabSolutions Software Screens
Learn more >>
Shimadzu offers the following training classes:

HPLC-101: Shimadzu Modular HPLC Training with PM
HPLC-102: Shimadzu i-Series HPLC Training with PM

Click here to learn more!

Shimadzu has launched its interactive "Virtual Advisor" websites to further enhance customer service.

>> Learn about the Nexera Virtual Advisor.

For Research Use Only. Not for use in diagnostic procedures.