Small Molecule Analysis

This study has been carried out for structural elucidation of paracetamol and its related impurities followed by quantitation of representative impurities using LCMS-9030, Q-TOF. The LCMS-9030 is built using a remarkably stable TOF tube iRefTOFTM, to deliver both high resolution and accurate mass with stability; attributes essential for compound identification, confident formula assignment and quantitation.

This report introduces and example of the structural analysis in which impurities detected under nonvolatile mobile phase conditions were converted into volatile mobile phase conditions on-line without any complicated examination using a trap free 2-dimensional (2D) HPLC and then subjected to precision mass spectrometry using the LCMS-9030 Q-TOF.

The comprehensive two-dimensional Nexera-e, offering exhaustive analysis of diverse compounds, and the Co-sense series, permitting high resolution and enrichment of specific fractions, make it possible to compare the overall compound patterns in the target sample. With this method, the two workflows consisting of the differential analysis of a sample with a complex matrix, and a detailed analysis of the compounds of those results to reveal differences, can be conducted simultaneously, quickly and conveniently. 

A comprehensive and highly sensitive application for the analysis of lipid mediators and their metabolites using a triple quadrupole mass spectrometer LCMS-8060 is presented here.  Note that a single chromatographic analysis is capable of separating a wide range of species such as hydrophilic metabolites tetranor-PGs and hydrophobic arachidonic acid and both positive and negative ions are detected. Method sensitivity was compared between the LCMS-8060 and a conventional LCMS-8050 system.

The mobile phases used in the two modes are completely different, and it is very troublesome to switch modes in an analysis. Here, we introduce separations by supercritical fluid chromatography (SFC) using various columns with different stationary phases.

This article introduces a sublimation/recrystallization method we developed for 9AA, which recrystallization was previously said to be difficult. Examples of MS imaging using this method are also introduced for low molecular weight metabolites (LMWMs) in a mouse liver.

This article introduces examples of detecting antiseptic components from commercially available oral care products using a benchtop MALDI-TOF mass spectrometer.

This application provides a complete solution for the characterization of vitamin E-TPGS polymer sample, which combines the user of MALDI-8020 and Polymerix polymer data analysis software.

This article introduces a sublimation/recrystallization method we developed for 9AA, which recrystallization was previously said to be difficult. Examples of MS imaging using this method are also introduced for low molecular weight metabolites (LMWMs) in a mouse liver.

The use of DHS-GC-MS with the HS-20 trap enabled analysis of trace level blood components. Using a standard mixture of volatile components, results were compared with those obtained using the HS-SPME method. The use of DHS permits detection of a wide range of volatile components, including alcohols, with high sensitivity.   These results demonstrate that this system offers superior sensitivity to that obtained using SMPE in the measurement of volatile components.

This Application Data Sheet presents the results of the analysis of glycolysis metabolites extracted from human embryonic stem cells using an MRM method file included in the Smart Metabolites Database. 

This article describes a difference spectrum method and sample condensation technique using the compact IRSpirit, a stable and sensitive compact FTIR instrument.

The identification of hypromellose acetate succinate was performed on the IRSpirit, using IR Pilot to guide the user through the required workflow. In this instance, an Identification Test with Diamond ATR on a powder sample was used to collect data, pick peaks and generate a report. IR Pilot provides a step-by-step interface that enables a non-expert immediate success with sample analysis.

This article examined the equimolar sensitivity characteristic for multiple sulfur compounds, using a Shimadzu Nexis™ SCD-2030. In addition, a comparison experiment was conducted with another selective and highly sensitive detector for sulfur the Flame Photometric Detector (FPD(S)).

In this application we describe an example of analysis that uses ion exclusion chromatography to increase separation selectivity for formic acid, acetic acid, fumaric acid, and maleic acid, which are organic acids frequently used for drug counterions.

Polymorphism is the phenomenon of materials with the same chemical structure having different crystalline structures. Differential scanning calorimetry (DSC) is an effective technique for investigating polymorphism that requires no sample pretreatment and performs measurements quickly. DSC can be used to evaluate polymorphism based on different crystal shapes having different melting points and heat of fusions. In our example we use DSC to analyze polymorphs.

The reaction time and activation energy of various types of reactions were obtained by reaction rate analyses. The progress of a reaction at desired points in time when a sample is held at a certain temperature can be determined by conducting an isothermal analysis, and the external energy necessary to induce the reaction can also be determined by obtaining the activation energy of that reaction. It is possible to study stable storage methods for samples under external environmental conditions such as light and temperature, and make predictions of the reaction rate of chemical reactions that occur over long periods of time.