Authenticity & Adulteration

LCMS-8045 Application - Highly Sensitive and Rapid Analysis of Synthetic Dyes in Sea Food by LC/MS/MS
Here, LC/MS/MS method has been developed for quantitation of malachite green, leucomalachite green, crystal violet and leucocrystal violet from sea food sample using LCMS-8045, a triple quadrupole mass spectrometer from Shimadzu Corporation, Japan.
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Differentiating Olive Oils Using UV-VIS Spectrophotometer and Spectrofluorophotometer
There is growing interest in food safety and organic foods, with an increasing number of shops specializing in a variety of food products and increasing circulation of high price import goods. One of these foods is olive oil, which is supposed to have both health and aesthetic benefits. The most expensive form of olive oil is extra virgin olive oil, which is regulated by the International Olive Council. Only olive oil that is chemically unprocessed, produced by squeezing and filtering olive fruit, and with an acidity of no more than 0.8 % qualifies as extra virgin olive oil. Another olive oil called pure olive oil is created by purification and hightemperature treatment. Differentiating between extra virgin olive oil and pure olive oil based on appearance alone is difficult. This article describes an attempt to differentiate between these two olive oil types by spectrum measurement using Shimadzu UV-2700 UV-VIS spectrophotometer and RF - 6000 spectrofluorophotometer, then performing multivariate analysis.
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High Sensitivity Analysis of Peanut Allergen in Cumin and Spice Mix [LCMS-8060]
Food allergens are a major public health concern. Among them, peanut allergy is one of the common food allergies. To avoid unexpected contact with food allergens, food labels are strictly used to indicate the presence of specific allergens. With the increasing awareness of food allergies, the presence of undeclared peanut in cumin lead to huge recalls in recent years. Although ELISA is the most commonly used technique to detect allergens, its false-positive rate is a major concern due to its cross-reactivity. We developed a method with high specificity and sensitivity to overcome this issue by using a high sensitivity triple quadrupole mass spectrometer to detect peanut allergen Ara h1 in commercially available spices and seasonings.
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LCMS-8060 Application - High Sensitivity Analysis of Peanut Allergen in Cumin and Spice Mix (Japan)
Food allergens are a major public health concern. Among them, peanut allergy is one of the common food allergies. To avoid unexpected contact with food allergens, food labels are strictly used to indicate the presence of specific allergens. With the increasing awareness of food allergies, the presence of undeclared peanut in cumin lead to huge recalls in recent years. Although ELISA is the most commonly used technique to detect allergens, its false-positive rate is a major concern due to its cross-reactivity. We developed a method with high specificity and sensitivity to overcome this issue by using a high sensitivity triple quadrupole mass spectrometer to detect peanut allergen Ara h1 in commercially available spices and seasonings.
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AIM-9000 Applciation - Simultaneous Measurement and Visual Observationv - Transmittance Measurement of Multilayer Film
By using the AIM-9000 infrared microscope together with AIMsolution Analysis software, measurement points can be visually observed at the same time as a spectrum is measured for the corresponding point. In this example, a multilayer film sample is analyzed using simultaneous visual observation and spectral measurement.
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LCMS-8030 Application - Comprehensive Two-dimensional Liquid Chromatography/Triple Quadrupole Mass Spectrometry: The Perfect Marriage (Japan)
This novel system combines the separation capabilities of comprehensive two-dimensional liquid chromatography (LC√óLC), and the specificity and sensitivity of triple quadrupole mass detection (MSMS). The hyphenation of the techniques generates a powerful analytical system, capable of extremely high-resolution power, as well as targeted and untargeted analysis, simultaneously. The so-called selected reaction monitoring (SRM) mode in fact enhanced selectivity, reducing sample consumption and the need for tedious clean-up procedures, specifically for beta-carotene quantification in a red chili pepper extract.
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Analysis of Commerical Honeys for Corn Syrup Adulteration by FTIR Spectroscopy
Honey is a valuable food commodity that gains a lot of attention due to its potential health benefits and usage to sweeten foods and beverages. To save on manufacturing costs, honey is susceptible to deliberate adulteration with sugar syrups such as corn syrup. Honey adulteration does not pose significant health problems, but it negatively influences market growth and consumer confidence. Fourier transform infrared spectroscopy (FTIR) with attenuated total reflectance (ATR) was used to collect absorption spectra of various commercial honey samples. Partial-least squares (PLS) was used to process FTIR spectra to determine the potential adulteration of honey samples. The techniques presented in this work provide and alternative to determine the purity and authenticity of foods, such as honey, and other natural products.
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LCMS-8040 Application - Quantitative Analysis of Illegal Dyes in Eggs Using LC/MS/MS (India)
A method for pre-treatment and subsequent LC/MS/MS analysis has been developed for highly sensitive quantitation of these dyes from egg using LCMS-8040, a triple quadrupole mass spectrometer from Shimadzu Corporation, Japan. Ultra-fast scanning speed of LCMS-8040 enables simultaneous analysis of four sudan dyes and para red dye. All these dyes were quantitated at low ppb levels with high specificity and good repeatability even in presence of egg matrix. Sudan and para red dyes consists of a group of synthetic industrial dyes like sudan I, II, III and IV etc. The International Agency for Research on Cancer evaluated the safety of sudan I, II, III and IV in 1987 and considered that they were unclassifiable as to their carcinogenicity to humans. Although there is some evidence that sudan dyes may cause cancer in experimental animals and may cause damage to the genes, there is currently inadequate evidence that they cause cancer in humans. as shown in Figure 3.
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LCMS-8050 Application - Cooked Meat Product Analysis by LCMS/MS to Determine Between Animal Species Using Proteotypic Peptide Quantitative Proteomic Analysis (UK)
With the emergence of new food safety challenges innovative analytical technologies are being developed to enable industry stakeholders and regulators to make sound scientific decisions. These advances have been made in response to changing regulations and needs and also to help maintain the safety of the food chain. In the case of unwanted or undeclared ingredients food authenticity testing is important component in many quality and safety programs. In 2013, horse meat issues received intense scrutiny and Polymerase Chain Reaction (PCR) techniques were used to screen for horse DNA in processed food samples. However, despite its widespread use in Europe for food fraud, PCR is problematic. DNA extraction kits are expensive and often require up to 8 h for the extraction, followed by cleanup and the PCR process. DNA is also prone to degradation especially in processed food. In contrast, mass spectrometry enables a promising approach to the detection of marker peptides in tryptic digests of horse, pig, beef and chicken with high sensitivity. In this work previously published proteotypic peptides were selected to detect horse, pork, beef and chicken using Skyline software (MacCoss Lab Software - University of Washington). These were used to interrogate Uniprot database FASTA download for myosin (43263 proteins) and collagen (44531 proteins) to determine no other species cross reactivity to pig myosin and collagen.
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Authenticity & Adulteration, Food Beverages & Other Consumer Products, Health, Dietary, Nutraceutical & Supplements
Here, carotenoids extracted from red chili pepper were subjected to two-dimensional analysis, in which mico-scale separation was conducted in the first stage using normal phase conditions, and separation using reversed phase conditions was tried in the second dimension. For detection, a combination of a photodiode array (PDA) connected to the LCMS-8030 triple quadrupole mass spectrometer was used. Because the separation modes, normal and reversed phases, differ in the first and second dimensions, this might be considered a two-dimensional LC method by which the greatest orthogonality possible is obtained.
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Nexera-e Application - Improved Analytical Workflow for Phospholipids by Nexera-e and Co-Sense for Impurities
Smart Metabolites Database registers MRM information of 475 metabolites mainly contained in biological samples such as blood, urine and cells. It enables simultaneous measurement of 475 metabolites using MRM mode. This application data sheet presents an analysis of metabolites in standard human plasma using the scan and MRM methods included in the Smart Metabolites Database, as well as a comparison of the results.
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i-Series Application - Analysis of Sugars and Sugar Alcohols in Energy Drink by Prominence-i with Differential Refractive Index Detector
This application note describes a GCMS purge-and-trap (P & T) method validation study conducted to evaluate operating conditions for the existing US EPA Method 624 VOC list, using updated technology and advanced GCMS instrumentation. GCMS instrument operating conditions are provided which bring the method in line with capabilities provided by contemporary laboratory equipment. This application note provides calibration results across three different ranges, complete MDL and Precision and Accuracy studies at multiple concentrations, and analysis of an independent laboratory VOC reference standard and real-world samples.
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i-Series Application - Analysis of Polystyrene with Antioxidant Additive Using Prominence-i GPC System
In this Application News, we introduce an accurate identification method for typical steroidal and non-steroidal anti-inflammatory drugs using multiple reference ion ratios, in addition to an example of high-sensitivity measurement.
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Nexera-e Application - Comprehensive Two-Dimensional Analysis of Polyphenols in Red Wine Using Nexera-e Coupled with SPD-M30A
Phenolic compounds are created as secondary metabolites in plants. In many cases, their structures contain multiple aromatic rings and a hydroxyl group. These polyphenols are known to display antioxidant effects, and are said to be effective in preventing arteriosclerosis and cerebral infarction. Red wine is known to contain large amounts of polyphenols, including flavonoids and phenolic acids, and in recent years has attracted considerable attention due to the health benefits associated with these substances. Regarding the analysis of polyphenols in red wine, separation and quantitation of these polyphenols by simultaneous analysis using HPLC is difficult due to the presence of many coexisting substances. The Nexera-e comprehensive two-dimensional liquid chromatograph can be very effective for such an analysis.
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ICP Application - ICP Analysis of Trace and Major Elements in Rice
Here, using the Shimadzu ICPE-9800 series multi-type ICP atomic emission spectrometer, we conducted content analysis of soil. The ICPE-9800 series, with its mini-torch plasma and spectrometer capable of simultaneous analysis of all elements at all wavelengths, can be used to conduct high-throughput, low cost analysis with high sensitivity and high precision.
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For Research Use Only. Not for use in diagnostic procedures.

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