Mass Spectrometry Imaging Data Analysis Software - IMAGEREVEAL MS

Mass Spectrometry Imaging Data Analysis Software - IMAGEREVEAL MS

Advances in Mass Spectrometry Imaging (MSI) hardware have enabled users to push the limits of pixel resolution while maintaining short acquisition times. One direct result of these advances is an exponential increase in datafile size and the number of experiments performed, driving the need for powerful image analysis software. Shimadzu's IMAGEREVEAL is a powerful software designed for processing of MSI files, enabling seamless analysis of large datasets. The software offers all the tools needed to interpret MSI datafiles qualitatively and quantitatively. 

 

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Workflow

Analysis parameters easily changed for optimal data processing output

Features

1. Processing in as few as 3 steps

 

Collective Analysis Mode

Automatically obtain MS images with distinctive characteristics using the "Collective Analysis" mode with pre-set parameters.
This highly convenient feature allows you to apply similar processing parameters to a group of datafiles. Create data matrix tables from differential analysis and/or image analysis, carry out statistical data analysis, and obtain MS images all in one step. 

Conventional Analysis Workflow vs Workflow with collective analysis 

 
 

Example Using a Real Data Set

Each feature of the software will be showcased using a real dataset. The selected dataset is part of a study related to Non-Alcoholic Steatohepatitis (NASH).

What is NASH?

NASH (Non-Alcoholic Steatohepatitis) refers to a fatty liver disease that is not related to alcohol consumption.

The livers of mice with nash are cut into serial sections, a stained image is created from the serial sections, ms imaging data is obtained from the serial sections

 

Using the "Collective Analysis" mode, MS images where the NASH tissue shows peculiarities with respect to normal tissue are automatically selected.

Sections to be analyzed and automatically selected MS images 

 

2. Multiple analysis modes

Choose  analysis settings using up to five pre-set methods. A single datafile can be processed using multiple methods. Select the best method for a given dataset after optimization.

 
  1. Finding molecules unique to tissue (Differential Analysis)
  2. Finding molecules with the same distribution as the stained image (Image Analysis)
  3. Creating an MS image showing the concentration distribution of the target molecule (Quantitative Analysis)
 
 

Example Using a Real Data Set

Finding molecules unique to NASH tissue (Differential Analysis)

Distinctive features between each region of interest of each type of tissue (NASH vs. Normal) can be found using statistical analysis.

 

MS images are obtained where NASH tissue and normal tissue show a different distribution

 
 
 

Finding molecules with a similar distribution as the stained image (Image Analysis)

IMAGEREVEAL includes powerful statistical tools that enable users to identify molecules with distinctive distributions.

 

Similar image extraction is a method to search through MS image data and find MS images showing similar distributions to the stained image

Further data analysis can be carried out using "Image Classification", "Segmentation", etc.

 
 

Creating an MS image showing the concentration distribution of a target molecule (Quantitative Analysis)

Using calibration curves, convert the intensity of a target molecule in the Region of Interest (ROI) into a concentration value.

An MS image is obtained showing the amount of the drug that has reached the liver

3. All MSI file formats supported using IMDX converter

Convert data files to the imdx format readable by IMAGEREVEAL using the included "IMDX Converter" tool.
The iMScope "kbd" format and general "Analyze 7.5" and "imzML" can be converted with this tool.

 

1) Obtain Data, 2) Convert to a general format, 3) Convert data to imdx format

4. Other features

 

Target analysis / Non-target analysis

  • Targeted Analysis: Review heat map of specific, targeted, molecules, m/z. 
  • Non-target Analysis: Review m/z over a specified mass range. 
 

Compound list

Access to a summary table listing all detected ions characteristics: mass-to-charge ratio, compound name and adducts.

Results based on the compound list

 

Simultaneous processing of multiple MS Image data files

IMAGEREVEAL users can process multiple imaging and non-imaging files at the same time, using the same processing and analysis method. Data analysis can be carried out on up to a few hundred GB without splitting up the data.

For Research Use Only. Not for use in diagnostic procedures.

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