Mass Spectrometry Imaging Data Analysis Software - IMAGEREVEAL MS
Advances in Mass Spectrometry Imaging (MSI) hardware have enabled users to push the limits of pixel resolution while maintaining short acquisition times. One direct result of these advances is an exponential increase in datafile size and the number of experiments performed, driving the need for powerful image analysis software. Shimadzu's IMAGEREVEAL is a powerful software designed for processing of MSI files, enabling seamless analysis of large datasets. The software offers all the tools needed to interpret MSI datafiles qualitatively and quantitatively.
Collective Analysis Mode
Automatically obtain MS images with distinctive characteristics using the "Collective Analysis" mode with pre-set parameters.
This highly convenient feature allows you to apply similar processing parameters to a group of datafiles. Create data matrix tables from differential analysis and/or image analysis, carry out statistical data analysis, and obtain MS images all in one step.
Example Using a Real Data Set
Each feature of the software will be showcased using a real dataset. The selected dataset is part of a study related to Non-Alcoholic Steatohepatitis (NASH).
What is NASH?
NASH (Non-Alcoholic Steatohepatitis) refers to a fatty liver disease that is not related to alcohol consumption.
Using the "Collective Analysis" mode, MS images where the NASH tissue shows peculiarities with respect to normal tissue are automatically selected.
Choose analysis settings using up to five pre-set methods. A single datafile can be processed using multiple methods. Select the best method for a given dataset after optimization.
- Finding molecules unique to tissue (Differential Analysis)
- Finding molecules with the same distribution as the stained image (Image Analysis)
- Creating an MS image showing the concentration distribution of the target molecule (Quantitative Analysis)
Example Using a Real Data Set
Finding molecules unique to NASH tissue (Differential Analysis)
Distinctive features between each region of interest of each type of tissue (NASH vs. Normal) can be found using statistical analysis.
Finding molecules with a similar distribution as the stained image (Image Analysis)
IMAGEREVEAL includes powerful statistical tools that enable users to identify molecules with distinctive distributions.
Further data analysis can be carried out using "Image Classification", "Segmentation", etc.
Creating an MS image showing the concentration distribution of a target molecule (Quantitative Analysis)
Using calibration curves, convert the intensity of a target molecule in the Region of Interest (ROI) into a concentration value.
Convert data files to the imdx format readable by IMAGEREVEAL using the included "IMDX Converter" tool.
The iMScope "kbd" format and general "Analyze 7.5" and "imzML" can be converted with this tool.
Target analysis / Non-target analysis
- Targeted Analysis: Review heat map of specific, targeted, molecules, m/z.
- Non-target Analysis: Review m/z over a specified mass range.
Access to a summary table listing all detected ions characteristics: mass-to-charge ratio, compound name and adducts.
Simultaneous processing of multiple MS Image data files
IMAGEREVEAL users can process multiple imaging and non-imaging files at the same time, using the same processing and analysis method. Data analysis can be carried out on up to a few hundred GB without splitting up the data.