LC/MS/MS Method Package for Cell Culture Profiling
For LabSolutions Version 5
Provides Simultaneous Analysis Conditions for 95 Components
These methods target culture medium components and metabolites secreted by cells. This culture medium analysis platform enables the simultaneous analysis of up to 95 components, the highest number of target analysis components* that can be analyzed by such a platform. Such features make it possible to acquire detailed data concerning cell culture profiles.
* Per survey results as of January 2015
Enables Simultaneous Analysis in 17 minutes
Compounds such as amino acids and vitamins are commonly analyzed by each compound group, which makes analysis of a culture medium time-consuming. By providing conditions for efficient and simultaneous multi-component analysis, this method package enables simultaneous analysis in 17 minutes.
Optimized Methods for the Analysis of a Culture Medium
Pre-set analysis conditions fully utilize the capabilities of LC-MS/MS for analyzing trace components such as vitamins. In addition, since there is no saturation of the signal with high-concentration components, such as glucose or amino acids, it is possible to measure a variety of culture medium components using the same vial.
Note: In order to gain even more accurate quantitative results, a dilution series needs to be created.
Ready-to-Use Methods Provided
Shimadzu Method Packages deliver conditions for efficient and simultaneous multi-component analysis. They enable the user to quickly and easily implement complex methods without costly and laborious method development by providing sample preparation protocols, LC separation conditions, and MS acquisition parameters.
List of Registered Compounds
|Internal Standard||Amino Acid and Derivatives||Vitamins|
|2-Isopropylmalic acid||2-Aminoadipic acid
Ascorbic acid 2-phosphate
|Nucleic acid associated compounds|
Remarks and Precautions
- For Research Use Only. Not for Use in Diagnostic Procedures.
- Shimadzu makes no warranty regarding the accuracy of information included in the database or the usefulness of information obtained from using the database.
- It is the user's responsibility to adopt appropriate quality control tests using standard samples to confirm qualitative and quantitative information obtained with this method package.