Exact Mass Database for Endogenous Metabolites
Ready-to-use methods for metabolomics
The physical properties of target compounds in metabolomics can vary greatly. An analysis method tailored to the target compounds is therefore needed for comprehensive measurements of metabolites.
This database contains multiple ready-to-use methods for comprehensive analysis of metabolites with different properties. Pretreatment examples are also included for various metabolites and samples. The user can begin a metabolomics analysis without time-consuming evaluation of LC or MS conditions.
Information for 470 compounds
This database is based on the successful LC/MS/MS Method Packages for Primary Metabolites, Cell Culture Profiling, Lipid Mediators, Short Chain Fatty Acids, and Bile Acids, and provides a comprehensive list of 470 metabolites (including internal standards) with retention times and exact mass information.
The database is compatible with the Multi-omics Analysis Package (sold separately). Processing the analysis results with the Multi-omics Analysis Package allows metabolic mapping, network analysis and projection on the metabolic map to be carried out easily.
|Method Name||No. of Registered Compounds*|
|Cell Culture Profiling||96|
|Short Chain Fatty Acids||23|
* Including internal standards
Total Solutions for Metabolomics
Shimadzu provides a set of workflows using a variety of mass spectrometers and database/method packages for metabolomics.
Example 1: Biomarker Discovery
Example 2: Comprehensive Cell Culture Profiling
- LabSolutions LCMS Ver. 5.97 or later is required.
- This method package is for research use only. Not for use in diagnostic procedures.
Total Solution for Metabolomic Analysis of Endogenous Metabolites Using Exact Mass Database
Metabolomics can target metabolites with a wide range of physical properties. Because of this, a comprehensive analysis of metabolites requires an analytical method and database suited to the target metabolites. This technical report describes the LC/Q-TOF exact mass database for endogenous metabolites and describes an example of applying this database as part of a total solution ranging from untargeted metabolomics on a Q-TOF LC-MS system to high-sensitivity widely targeted metabolomics on a triple quadrupole LC-MS system for analyzing metabolites in an iPS cell culture medium.