LC/MS/MS Method Package for Primary Metabolites Ver.3

LC/MS/MS Method Package for Primary Metabolites Ver.3

Ready-to-use methods for 200 compounds

This Method Package enables efficient, simultaneous analysis of a large number of compounds. Optimized LC separation conditions and MS parameters reduce the time and effort expended on method development.

 

Choice of optimized analysis conditions

Choose between two method types to suit your analysis aims and equipment. The ion-pair reagent method (112 compounds) is particularly useful for the analysis of sugar phosphates, while the non-ion-pair reagent method (141 compounds), effective in the research of high-value-added substances, does not require ion-pair reagents and in this version has been expanded to include compounds involved in the mevalonate and shikimic acid pathways.

 
Overlaid MRM chromatograms for simultaneous analysis of a mixture of 141 standards with the non-ion-pair reagent method

Overlaid MRM chromatograms for simultaneous analysis of a mixture of 141 standards with the non-ion-pair reagent method

 
 

Visualization of metabolic changes

With the included Multi-omics Data Analysis Package, quantitative data can be visualized easily on a metabolic map.

Visualization of simultaneous analysis results from the PFPP column method, created with the Multi-omics Data Analysis Package

Visualization of simultaneous analysis results from the PFPP column method, created with the Multi-omics Data Analysis Package

 
 

Compatible with the Nexera™ series and the LCMS-8060NX

All methods are compatible with both the Nexera series and the LCMS-8045/8050/8060 (NX), covering a wide range of analysis needs.

 

A total solution from pretreatment to analysis

This Method Package includes protocols for preparing biological tissue extracts, enabling stable analysis with proven pretreatment techniques and reducing the labor and expense involved in method development.

Index of Compounds

List of compounds for ion-pair reagent method
Glycolytic system Co-enzyme Nucleotides
2,3-Bisphosphoglyceric acid
3-Phosphoglyceric acid
(2-Phosphoglyceric acid)
Dihydroxyacetone phosphate
Fructose 1,6-bisphosphate
Glucose 1-phosphate
Glucose 6-phosphate
Glycerol 3-phosphate
Phosphoenolpyruvic acid
Pyruvic acid
3-Hydroxybutyryl coenzyme A
Butyryl coenzyme A
Coenzyme A
Crotonyl coenzyme A
FAD
FMN
Malonyl coenzyme A
Methylmalonyl coenzyme A
NAD
NADH
NADP
NADPH
Nicotinic acid
Pyrroloquinoline quinone
Adenine
Adenosine
Adenosine 3',5'-cyclic monophosphate
Adenosine diphosphate
Adenosine monophosphate
Adenosine triphosphate
AICAR
Cytidine
Cytidine diphosphate
Cytidine monophosphate
Cytidine triphosphate
Guanine
Guanosine
Guanosine 3',5'-cyclic monophosphate
Guanosine diphosphate
Guanosine monophosphate
Guanosine triphosphate
Inosine
Inosine monophosphate
Orotic acid
Thymidine
Thymidine diphosphate
Thymidine monophosphate
Thymidine triphosphate
Thymine
Uridine
Uridine diphosphate
Uridine monophosphate
Uridine triphosphate
Xanthosine monophosphate
Pentose-phosphate pathway
Fructose 6-phosphate
Glyceraldehyde 3-phosphate
6-Phosphogluconic acid
Erythrose 4-phosphate
Ribose 1-phosphate
Ribose 5-phosphate
Ribulose 5-phosphate
Sedoheptulose 7-phosphate
Sugar phosphate Nonmevalonic acid pathway
Fructose 1-phosphate
Mannose 6-phosphate
Phosphoribosyl pyrophosphate
Ribulose 1,5-bisphosphate
HMBPP
IPP_DMAPP
MEP
TCA cycle Shikimic acid pathway
Acetyl coenzyme A
2-Ketoglutaric acid
Succinyl coenzyme A
Shikimic acid
Shikimic acid 3-phosphate
Amino acids Organic acids Nucleotide sugar
2-Aminobutyric acid
4-Aminobutyric acid
Alanine
Arginine
Asparagine
Aspartic acid
Cysteine
Glutamic acid
Glutamine
Glycine
Histidine
4-Hydroxyproline
Isoleucine
Leucine
Lysine
Methionine
Phenylalanine
Proline
Serine
Threonine
Tryptophan
Tyrosine
Valine
2-Isopropylmalic acid
3-Hydroxyphenylacetic acid
4-Hydroxyphenyl pyruvic acid
Citramalic acid
Glyceric acid
Glycerol 2-phosphate
Glycerol 3-phosphate
Glycolic acid
Indole 3-acetic acid
Pantothenic acid
ADP-glucose
UDP-glucose
Purine derivative
Hypoxanthine
Uric acid
Xanthine
Internal STDs
2-Morpholinoethanesulfonic acid
Methionine sulfone
 
List of compounds for PFPP column method
Glycolytic system Organic acids Co-enzyme
Lactic acid
Pyruvic acid
2-Aminobutyric acid
4-Aminobenzoic acid
4-Aminobutyric acid
Caffeic acid
Cholic acid
Creatine
Ferulic acid
Glycolic acid
Glyoxylic acid
Ophthalmic acid
Orotic acid
p-Coumaric acid
Phenyllactic acid
Phenylpyruvic acid
Taurocholic acid
Urocanic acid
Vanillic acid
FAD
FMN
NAD
Niacinamide
Nicotinic acid
TCA cycle Nonmevalonic acid pathway
2-Ketoglutaric acid
Aconitic acid
Citric acid
Fumaric acid
Isocitric acid
Malic acid
Succinic acid
DOXP
MEP
Urea cycle Catecholamine Mevalonic acid pathway
Argininosuccinic acid
Ornithine
Citrulline
Dopamine
Epinephrine
Norepinephrine
Serotonin
Mevalonic acid
MVA-P
Amino acids Vitamin B Alkaloid
4-Hydroxyproline
Alanine
Anthranilic acid
Arginine
Asparagine
Aspartic acid
Asymmetric dimethylarginine
Cystine
Dimethylglycine
Glutamic acid
Glutamine
Glycine
Histidine
Homocystine
Isoleucine
Leucine
Lysine
Methionine sulfoxide
Phenylalanine
Proline
Serine
Symmetric dimethylarginine
Threonine
Tryptophan
Valine
Folic acid
Pantothenic acid
PLP
Higenamine
Reticuline
THP
Nucleosides and Nucleotides Others
Adenine
Adenosine
Adenosine 3',5'-cyclic monophosphate
Adenosine monophosphate
Adenylsuccinic acid
AICAR
Cytidine
Cytidine 3',5'-cyclic monophosphate
Cytidine monophosphate
Cytosine
Guanine
Guanosine
Guanosine 3',5'-cyclic monophosphate
Guanosine monophosphate
Inosine
Thymidine
Thymidine monophosphate
Thymine
Tyrosine
Uracil
Uridine
4-Aminophenylalanine
4-Aminophenylpyruvic acid
4-Hydroxybenzoic acid
Acetylcarnitine
Acetylcholine
Carnitine
Carnosine
Catechol
Choline
Citicoline
Creatinine
Cysteamine
Dihydroxyphenylacetaldehyde
Dihydroxyphenylacetic acid
Dopa
Ergothioneine
Histamine
Histidinol
Hydroxytyrosol
Indole
Kynurenine
Methyl-DOPA
Protocatechuic acid
Protocatechuic aldehyde
Resveratrol
Salicylic acid
Sinapic acid
Tyramine
Vanillin
Methylation and Transsulfuration cycle Purinederivative Internal STDs
Cystathionine
Cysteine
Homocysteine
Methionine
5-Glutamylcysteine
Glutathione
Oxidized glutathione
S-Adenosylhomocysteine
S-Adenosylmethionine
Allantoin
Hypoxanthine
Uric acid
Xanthine
2-Morpholinoethanesulfonic acid
Methionine sulfone
Shikimic acid pathway
3-Dehydroquinic acid
3-Dehydroshikimic acid
Chorismic acid
Shikimic acid
Shikimic acid 3-phosphate
  •  With this method package, choose between the ion-pair reagent method (112 compounds) or the PFPP column method (141 compounds) depending on your equipment and analysis aims.

Precautions

  1. LabSolutions LCMS Ver.5.99 SP2 or later is required.
  2. This method package is for research use only.

LabSolutions and Nexera are trademarks of Shimadzu Corporation.
GARUDA is a trademark of The Systems Biology Institute.

Applications

 

Food Metabolomics Analysis of Deterioration Characteristics of Alcoholic Drinks Using LC/MS/MS

Food Metabolomics Analysis of Deterioration Characteristics of Alcoholic Drinks Using LC/MS/MS PDF

This report describes an analysis method used to determine the deterioration characteristics of foods based on food metabolomics. The samples, commercially available Japanese rice wine (sake) and white wine, were stored under adverse conditions and then separated by high performance liquid chromatography mass spectrometry (LC/MS/MS), followed by multivariate analysis, to comprehensively investigate the changes in hydrophilic metabolites, including amino acids, organic acids, nucleosides, and nucleotides.

Read More 

 

Metabolome Analysis of Japanese Rock Ptarmigan Feces by LC/MS/MS: Application to the Establishment of Breeding Technology for Endangered Species

Metabolome Analysis of Japanese Rock Ptarmigan Feces by LC/MS/MS PDF

In this article, feces containing metabolites produced by the intestinal flora of wild and artificially bred Japanese rock ptarmigans were analyzed using LC/MS/MS. It was suggested that metabolome analysis could be applied in establishing breeding technology focused on return to the wild, so we are introducing an example of that here.

Read More 

 

Food Metabolomics Evaluation of Japanese Rice Wine Types Using LC/MS/MS

Food Metabolomics Evaluation of Japanese Rice Wine Types Using LC/MS/MS PDF

As an example of food metabolomics, this article introduces an analysis of five types of specially designated Japanese rice wine (sake). In this study, we comprehensively analyzed the hydrophilic components of sake samples with a high performance liquid chromatograph mass spectrometer (LC/MS/MS) and verified the characteristics of each sake type.

Read More 

 

Food Metabolomics Analysis of Curry Using LC/MS/MS

Food Metabolomics Analysis of Curry Using LC/MS/MS PDF

This article covers an example of food metabolomics where we examined the changes observed when different ingredients are used in curries made in several households, and when a curry is left overnight, by comprehensively analyzing the hydrophilic components of the sauce using a high performance liquid chromatograph mass spectrometer (LC/MS/MS) and conducting verification through multivariate analysis.

Read More 

 

A Multiomics Approach Using Metabolomics and Lipidomics

A Multiomics Approach Using Metabolomics and Lipidomics PDF

By applying the approaches of metabolomics and lipidomics, this article introduces an example of evaluating how related sulfur-containing metabolites change depending on cultivation progression.

Read More 

Remarks and Precautions

  1. For Research Use Only. Not for Use in Diagnostic Procedures.
  2. Shimadzu makes no warranty regarding the accuracy of information included in the database or the usefulness of information obtained from using the database.
  3. It is the user’s responsibility to adopt appropriate quality control tests using standard samples to confirm qualitative and quantitative information obtained with this method package.

For Research Use Only. Not for use in diagnostic procedures.

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