GPC System Configuration
This page shows a typical example of a Gel Permeation Chromatography (GPC) System configuration.
A differential refractive index detector, which offers comparatively uniform mass sensitivity, is used as the GPC detector.
PEEK tubing is often used as the connections for reverse-phase analysis. However, stainless-steel tubing is used for gel permeation chromatography of synthetic polymers. This is because stainless-steel tubes are more corrosion-resistant to THF eluent under high pressure than PEEK tubes.
**The model to the right for the SIL should read: SIL-20AHT
Description of Configuration
|Product Name or Model||Description|
|CBM-20Alite||Card-type system controller. Built into LC-20AD or SIL-20A HT . Controls up to 5 units, including the one it is installed in.|
|LC-20AD||High-precision solvent delivery unit.|
|DGU-20A3||3-way online Degassing Unit.|
|Reservoir tray||Tray for eluent bottles.|
|SIL-20A HT||Rack for 1.5 mL sample bottles supplied as standard.
Simultaneously purchase Vespel needle seal, 1.5 mL glass vials, etc.
|RID-10A||Highly stable differential refractive index detector with dual temperature control.|
|LC workstation||Computer set (LCsolution Single). Also requires color printer, printer cable, etc.|
|GPC software||This is LCsolution optional software.|
|Shim-pack GPC-80M + Shim-pack GPC-800P guard column is an example of columns for THF. Select the appropriate columns according to the measured mass range and solvent used.|
Description of Gel Permeation Chromatography
Size-exclusion chromatography (What is this?) is a separation mode that separates the molecules by size, and falls into two types: non-aqueous GPC and aqueous GFC. It provides molecular weight distribution information about how much of each molecular weight is included. Consequently, it is widely used for quality control applications to determine differences in polymer properties.