AccuSpot LC-MALDI Plate Spotter
Automated LC-MALDI Spotting & Fraction CollectionBringing LC to MALDI technology
LC/MS/MS is often regarded as the technique of choice in identifying components from complex mixtures; however, it is not without its limitations. An alternative approach is to consider LC/MALDI. LC/MALDI has a number of advantages in that the LC is decoupled from the MALDI mass spectrometer. MALDI can support:
- A higher data acquisition at the top of the eluting peak allows for a precise selection of precursor ions for MS/MS analysis
- Sample archiving (the MALDI plate can be stored and used to further validate protein identification if required)
- Uncompromised sensitivity enables increased depth of coverage of the sample and enhanced sequence coverage for the detected proteins
The AccuSpot was designed for online collection of fractions eluting from Capillary/Nano LC. It automates LC micro-fractionation, spotting and preparation for MS analysis. With the AccuSpot, LC eluent can be accurately and continuously spotted onto target plates, in trace amounts (eg.50nl). This allows for automatic preparation of target plates for MALDI-TOF-MS measurements. When used in conjunction with Shimadzu's Two-Dimensional Micro LC System and Shimadzu’s AXIMA MALDI-TOF mass spectrometers, proteome analysis can be performed with a higher degree of precision and with greater sequence coverage.
Unique sample spotting mode
The "Half-Contact" spotting method (see above) uses plate sensors (patent pending) to ensure accurate spotting and virtually eliminates carryover from spot to spot.
High sample capacity
Multiple plates can be spotted continuously. It enables spotting on up to nine microtiter plates in 96 or 384 format of 18 2x2 (ABI).
Highly flexible sample loadings
Matrix solution is added automatically. In addition, The the matrix solution can be added at flow rates from 0.1µL/min to 50 µL/min. To accommodate different needs the spotting direction can be either straight row and column or zigzag row and column.
To ensure that the droplet formation and collection is consistent, a CCD camera is used to monitor micro-fractionation.
Spotting is carried out in one of two ways:
- Continuous Mode: to collect the entire chromatographic run
- Programmable Time Window Mode: to collect only the areas of interest in the chromatographic run
Both modes allow spotting intervals from 5 to 60 sec/spot.
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Easy to use software gives the user total control over the spotting operation from setting matrix flow rate to spotting pattern to report generation, while an optional barcode reader is available for sample tracking.