Principle of MALDI/TOFMS
What is MALDI?
MALDI is the abbreviation for "Matrix Assisted Laser Desorption/Ionization."
The sample for MALDI is uniformly mixed in a large quantity of matrix.
The matrix absorbs the ultraviolet light (nitrogen laser light, wavelength 337 nm) and converts it to heat energy. A small part of the matrix (down to 100 nm from the top outer surface of the Analyte in the diagram) heats rapidly (in several nano seconds) and is vaporized, together with the sample.
What is TOF MS?
TOF MS is the abbreviation for Time of Flight Mass Spectrometry.
Charged ions of various sizes are generated on the sample slide, as shown in the diagram. A potential difference V0 between the sample slide and ground attracts the ions in the direction shown in the diagram. The velocity of the attracted ions v is determined by the law of conservation of energy. As the potential difference V0 is constant with respect to all ions, ions with smaller m/z value (lighter ions) and more highly charged ions move faster through the drift space until they reach the detector. Consequently, the time of ion flight differs according to the mass-to-charge ratio (m/z) value of the ion. The method of mass spectrometry that exploits this phenomenon is called Time of Flight Mass Spectrometry.
Digital Ion Trap Mass Spectrometer
Despite its light and compact shape, the MALDImini-1 allows very high-sensitivity MSn measurements, making it suitable for a large number of applications.
Benchtop Linear MALDI-TOF Mass Spectrometer
The MALDI-8020 is the latest in a long line of MALDI-TOF products from Shimadzu. This benchtop, linear MALDI-TOF mass spectrometer delivers outstanding performance in a compact footprint, making it an ideal choice for today’s increasingly demanding laboratories.
News / Events
Shimadzu at ASMS 2022
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Benchtop MALDI-TOF Mass Spectrometer Featuring Integrated MegaDalton Ion Detection
Shimadzu Scientific Instruments, in collaboration with CovalX Instruments Inc., introduces the OmegaTOF benchtop high mass matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometer. The OmegaTOF system couples Shimadzu’s benchtop MALDI-TOF instrument platform (MALDI-8020) with established technology for high mass detection from CovalX. The result is a user-friendly, compact platform that extends benchtop MALDI-TOF analysis to a mass range of 1.5 MegaDalton.
New Benchtop MALDI-TOF Mass Spectrometer Features Dual-Polarity Ion Source
Shimadzu Scientific Instruments introduces the MALDI-8030 benchtop linear matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometer. Like its predecessor, innovative instrument design enables the MALDI-8030 to achieve performance specifications similar to those of larger, more expensive MALDI-TOF models in a significantly smaller footprint. This user-friendly compact instrument expands on the successful benchtop format of the MALDI-8020 by offering both positive-ion and negative-ion analysis.