Profiling Trace Volatile Compounds in Blood by Gas Chromatograph Mass Spectrometry with Dynamic Headspace Extraction

Various types of volatile substances are present in blood, and in addition to those that play roles in the formation of adducts such as proteins and nucleic acids, some are known to act as secondary messengers that regulate various biological functions. To learn more about these roles, the profiling of volatile components in blood has been attracting attention. Up to now, solid phase micro-extraction (SPME) and HS-SPME have been the primary methods used for analysis of volatile substances. The HS-SPME method is a static method in which a fiber coated with a solid (sorbent) extraction phase is exposed to the gas phase. Because extraction proceeds based on the equilibrium migration of volatile components, those volatile components that exhibit strong interaction with the extraction fiber present a strong profile. In this report, we focused on the dynamic headspace (DHS) method. The DHS method is a dynamic extraction method in which the gas phase is forcibly purged using an inert gas, and the volatile components are collected on the adsorbent. Since nearly all of the gas phase components can be collected, volatile components are efficiently collected, enabling a wider range of components to be analyzed by this method compared with conventional methods. Using a standard mixture of volatile substances, a comparison of the results of volatile substance profiling obtained using the HS-SPME and DHS methods showed that application of the DHS method permitted detection of all the volatile substances, including the alcohols, which were difficult to profile by HS-SPME. Furthermore, the results of validation of the DHS method confirmed the detection of all of the volatile components at ng levels. The results obtained applying this system with IL-10 knockout mice plasma confirmed the detection of 40 volatile compounds, in which a significant difference between samples was observed with respect to 15 compounds. High-sensitivity volatile substance profiling is possible using this system, and is expected to be applied in future clinical research.

Content Type:
Technical Report
Document Number:
C146-E291
Product Type:
Gas Chromatography Mass Spectrometry (GC-MS)
Keywords:
dynamic, headspace, trace volatile compounds, blood, extraction, Pharma & Biopharma, Small Molecule Analysis, GCMS-TQ8040
Language:
English
File Name:
C146-e291-Profiling-Trace-Volatile-Compounds-Blood-Gas-Chromatograph-Mass-Spectrometry-Dynamic-Headspace-Extraction.pdf
File Size:
326kb

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