A Data-independent Acquisition (DIA) Approach on Q-TOF Mass Spectrometry for In-depth Peptide Mapping of Monoclonal Antibodies

Peptide mapping plays a critical role in quality assurance of mAbs and is widely applied for primary structure characterization. However, we have to understand that peptide mapping is usually performed in a comparative manner, in which the peptide map of a proposed mAb product is compared to that of a reference product in a side-by-side experiment, which is extremely time consuming and highly dependent on the reproducible sample preparation. An in-depth peptide mapping method is thus required to characterize peptide sequences of mAbs. An emerging technique, and the focus of this report, is DIA mode on Q-TOF mass spectrometry. DIA enables a genetic, unbiased approach for the MS/MS data acquisition, in which all the ions in each individual MS/MS event are fragmented without precursor ion isolation and all the fragment ions are measured in a mixed spectrum, ideal for peptide sequencing [1]. In this study, we demonstrated an integrated DIA approach on LCMS-9030 QTOF mass spectrometer for de novo peptide sequencing of mAbs.

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Document Number:
ASMS 2020 - MP 117
Product Type:
Liquid Chromatography Mass Spectrometry (LC-MS)
Pharma & Biopharma, LCMS-9030 Q-TOF Mass Spectrometer
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