Glargine and its active metabolites (M1 and M2) were analyzed in human plasma using a solid phase
extraction method in MRM mode on LCMS-8060. The developed method is novel, simple and sensitive
for simultaneous estimation of glargine and its active metabolites in human plasma. Insulin Glargine is a recombinant human insulin analog with long-acting, blood glucose-lowering activity.
Insulin glargine differs from human insulin by replacing asparagine with glycine in position 21 of the Achain
and by carboxy-terminal extension of B-chain by 2 arginine residues. After subcutaneous injection,
glargine undergoes an enzymatic removal of the basic arginine pair at positions 30B and 31B to yield 21AGly-
human insulin (metabolite 1 [M1]), analogous to prohormone activation, with some further loss of
threonine to 21A-Gly-des-30B-Thr-human insulin (metabolite 2 [M2]).
- Content Type:
- Document Number:
- ASMS 2020 - TP 479
- Product Type:
- Liquid Chromatography Mass Spectrometry
- Life Science Solutions, LCMS-9030 Q-TOF Mass Spectrometer
- File Name:
- File Size:
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