A Fast and Comprehensive Potency Determination of 21-Cannabinoids in Hemp Material and Finished Tinctures using the Cannabis Analyzer for Potency

Cannabis is a plant of the Cannabaceae family and contains more than one hundred biologically active chemical compounds. The most commonly known compounds are delta-9- tetrahydrocannabinol (THC) and cannabidiol (CBD). THC is the component that produces the “high” associated with marijuana use. Much interest has been seen around CBD and its potential related to health benefits. Since 2018, the FDA has approved one CBD-containing drug Epidiolex, to treat two rare and severe forms of epilepsy. The same year, the 2018 Farm Bill was signed into law. Hemp is defined as product with no more than 0.3 percent THC on a dry weight basis. Currently the potency for hemp oil is reported in concentration units of mg/mL. For effective results, individuals’ prescriptions are different. Doses can be as low as 0.5-10 mg CBD per day, in other cases 100-200 mg CBD daily, or as high as 300-800 mg CBD daily in clinical trials. Tinctures are concentrated liquids commonly in quantities of 0.5, 1, or 2 ounces, with a dropper for measuring a specific number of drops (25-30 drops = 1 mL). The label shows the potency or mg of CBD per mL of solution. The research shows that the “entourage” effect of hemp makes it an effective therapeutic. There are conditions that respond to pure CBD, but many cases in which CBD is more effective in a broad-spectrum oil. In this application note, an HPLC method is presented which builds on the well-established potency method using the Shimadzu Cannabis Analyzer for Potency™, a comprehensive and fast determination of 21-cannabinoids in only 15 minutes (including the wash-step). Cannabinoid profiles for commercially available dry hemp and finished tinctures are presented.

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High Performance Liquid Chromatography (HPLC & UHPLC)
Potency Determination, Cannabinoids, Hemp Material, Finished Tinctures, Cannabis & Hemp Testing Solutions, Cannabis Analyzer for Potency
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For Research Use Only. Not for use in diagnostic procedures.

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