Monitoring the differentiation process of human iPS cells to cerebral cortical neurons by LC-MS/MS media analysis

Human induced pluripotent stem cells (hiPSCs) can differentiate into any cell type in the body. They consequently hold great promise as a source of cells for applications in regenerative medicine and drug discovery. To contribute to development of hiPSCs technologies, we have aimed to monitor the hiPSCs differentiation through multi components analysis using triple quadrupole LCMS/ MS. Alzheimer’s disease (AD) is a neurological disorder in which the death of brain cells causes memory loss and cognitive decline. Due to increasing of the number of patients with AD, drug development for AD has been requiring. To facilitate drug development for AD, human induced pluripotent stem cells (hiPSCs) has drawn attentions because disease-specific cells from patients can be used for in vitro model of AD by differentiation into cerebral cortical neurons. However, it needs long term culture process to differentiate hiPSCs into cerebral cortical neurons, there is no verification method during differentiation without cell disruption. Therefore, we have aimed to monitor the differentiation through multi components analysis using triple quadrupole LCMS/ MS.

Content Type:
Poster
Document Number:
ASMS 2020 - ThP 003
Product Type:
Liquid Chromatography Mass Spectrometry
Keywords:
Clinical Research, LCMS-8050 Triple Quadrupole Liquid Chromatograph Mass Spectrometer (LC-MS/MS)
Language:
English
File Name:
ThP003-ASMS-2020-Poster.pdf
File Size:
894kb

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