The purpose of this study is qualitative analysis and quantitation of a
phosphorothioate antisense oligonucleotide (Figure 1) using a LC/QTOF-
MS. The molecular weight confirmation and the sensitive
quantitation are successfully achieved by charge state deconvolution
and high-resolution MRM , respectively. With the current diversification of variety of oligonucleotide
therapeutics, there is an urgent need to improve the efficiency of
analytical method development. MS detection is easier way to analyze
oligonucleotide in both sample preparation and method development
comparing with immune-based detection such as ELISA, and also the
only methodology that can distinguish slight difference of modifications
and quite resembled impurities based on accurate M.W. information.
Here, we report quantitative and qualitative analysis of modified
antisense oligonucleotides in a single system of Q-TOF that combines
selectivity with sensitivity.
- Content Type:
- Document Number:
- ASMS 2020 - WP 459
- Product Type:
- Liquid Chromatography Mass Spectrometry
- LCMS-9030 Q-TOF Mass Spectrometer
- File Name:
- File Size:
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