Qualification and Quantitation of Phosphorothioate Oligonucleotides Using a Quadrupole-Time-of-Flight Mass Spectrometer

The purpose of this study is qualitative analysis and quantitation of a phosphorothioate antisense oligonucleotide (Figure 1) using a LC/QTOF- MS. The molecular weight confirmation and the sensitive quantitation are successfully achieved by charge state deconvolution and high-resolution MRM , respectively. With the current diversification of variety of oligonucleotide therapeutics, there is an urgent need to improve the efficiency of analytical method development. MS detection is easier way to analyze oligonucleotide in both sample preparation and method development comparing with immune-based detection such as ELISA, and also the only methodology that can distinguish slight difference of modifications and quite resembled impurities based on accurate M.W. information. Here, we report quantitative and qualitative analysis of modified antisense oligonucleotides in a single system of Q-TOF that combines selectivity with sensitivity.

Content Type:
Document Number:
ASMS 2020 - WP 459
Product Type:
Liquid Chromatography Mass Spectrometry
LCMS-9030 Q-TOF Mass Spectrometer
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