Untargeted LC-MS/MS-based metabolic phenotyping applied to the CD248 knock out mouse model

Untargeted LC-MS/MS is a powerful tool by which to identify metabolic phenotypes. Here we applied metabolic phenotyping analysis to the CD248 knockout mouse model. CD248 is a transmembrane glycoprotein, expression of which is markedly upregulated in a considerable number of disease models including tumor growth, inflammation and injury-induced fibrosis. In human clinical studies, CD248 expression is upregulated in the fat cells of patients with diabetes, conversely, CD248 expression reverted to a normal range when obesityassociated diabetes was reversed through weight loss. In this work, an untargeted LC-MS/MS metabolic phenotyping analysis, using a reverse-phase LC separation and high resolution accurate mass (HRAM), was applied to a CD248-/-mouse model following high fat diet (HFD) feeding to study the effects of diet on serum metabolite profiles.

Document Number:
ASMS 2020 - ThP 368
Product Type:
Liquid Chromatography Mass Spectrometry
Life Science Solutions, LCMS-9030 Q-TOF Mass Spectrometer
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