Development of cell culture supernatant analysis using LC-MS/MS and their application for Chinese hamster ovary cell

Optimization of cell culture process is crucial to improve the productivity and quality of biopharmaceuticals. Infrared spectroscopy and biosensor are used for analyzing cell culture fluid during culture process. While these methods are simple and short in measurement time, the measurement items are limited to pH, dissolving gases, and major small compounds such as glucose, glutamine, and lactic acid. Therefore, a simultaneous analysis method including trace components such as vitamins in the culture medium and secreted metabolites has been required for examining culture process in detail. We have developed simultaneous analysis method up to 125 compounds in 17 minutes using LC-MS/MS. In this poster, we present features of our developed method and its applications for Chinese hamster ovary cell.

Content Type:
Poster
Document Number:
ASMS 2020 - MP 423
Product Type:
Liquid Chromatography Mass Spectrometry
Keywords:
ASMS 2020, lcms, cell culture, supernatant, lcms, chinese, hamster, ovary cell, LCMS-8060
File Name:
MP423-ASMS-2020-Poster.pdf
File Size:
1,131kb

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