Highly sensitive analysis of the related substance of ciguatoxins by the multiple reaction monitoring and electrospray ionization with LC/MS/MS

Ciguatoxins (CTXs) are known as the seafood poisoning from fish around the tropical region. They have approximately 100 times stronger toxicity than puffer fish poison (Tetrodotoxin). Although one fish contains a very little amount of them each, many related substances among CTXs have been discovered. Thus, highly sensitive and selective analytical methods are strongly required for the determination. In previous reports, the sodium adducted ions have been used to determine by LC/MS/MS (ESI). In this study, we attempted to analyze five ciguatoxins (CTX1B, 52-epi-54-deoxyCTX1B, 51-hydroxyCTX3C, CTX3C, CTX4A) using multiple reaction monitoring. We designed the transition for MRM of each CTXs considering the product ions by collision-induced dissociation (CID) from protonated or ammonium adduct ions. We have developed the conditions in order to detect CTXs with high sensitivity and selectivity.

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Document Number:
ASMS 2020 - MP 205
Product Type:
Liquid Chromatography Mass Spectrometry (LC-MS)
ASMS MP205, ciguatoxins, mrm, electrospray, lcms/ms, LCMS-8060, LCMS-9030, Nexera X2 UHPLC
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