Ghost Trap DS - Applications
Mobile phase cleaner for HPLC
Consistently traps impurities, even in organic solvents
A major feature of the Ghost Trap DS is the ability to remove impurities even in organic solvents. When the Ghost Trap DS is installed between the gradient mixer and autosampler in reversed phase gradient analysis, it can trap impurities from the piping and gradient mixer in addition to those from the mobile phase.
The example below shows that it can effectively trap impurities in mobile phase, when the Ghost Trap DS is installed just downstream of the gradient mixer as displayed in the figure on the right.
|Mobile phase||A: 25 mmol/L Phosphate (Potassium) buffer solution pH 4.0 /Acetonitrile =9/1
B: Water/Acetonitrile = 1/9
|Flow rate||0.65 mL/min|
|Detection||UV 210 nm|
Application to Quantitative Analysis of Impurities Using the Nexera UHPLC
Quantitation of a small peak covered by ghost peaks can be difficult. Effective for the quantitative analysis of impurities, the Ghost Trap DS/DS-HP effectively eliminates such ghost peaks to ensure highly accurate quantitation, even with very small target component peaks.
|Mobile phase A||5 mmol/L phosphoric acid (sodium) buffer solution (pH 2.6)|
|Mobile phase B||5 mmol/L phosphoric acid (sodium) buffer solution (pH 2.6) / Acetonitrile = 1/3|
|Gradient||0.1 % (0 min) → 20 % (10 min) → 60 % (15 min) → 95 % (20 min)|
|Flow rate||0.5 mL/min|
|Injection volume||2 μL|
|Column||Shim-pack XR-ODSⅢ 75 mmL. x 2.0 mmI.D., 1.6 μm|