LC/MS/MS MRM Library for Phospholipid Profiling
For LabSolutions™ LCMS
This MRM library includes two methods: one for phospholipid classification by comprehensive analysis of the main phospholipids in biological samples, and one for fatty acid composition determination created using analytical results obtained with the classification method. The library targets phospholipids containing C14 to C22 fatty acids, and includes MRM transitions for up to 867 components. This library enables performing phospholipid profiling by conducting an initial analysis with a phospholipid classification method. This is followed by creating a method for fatty acid composition determination based on the phospholipid peak detected in the first analysis, and subsequently using this method to perform a second analysis to determine fatty acid composition.
The phospholipids registered in the MRM library have fatty acid compositions with the carbon number and double bond combinations shown in the table below. The phospholipid targets of the library are phosphatidylcholines (PC) with a lyso-group, phosphatidylethanolamines (PE), phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidylserines (PS), and sphingomyelins (SM).
MRM Transition Composition
Focusing on the characteristic phospholipid head group, the library includes a method for phospholipid classification and a method for fatty acid composition determination (for fatty acid compositions of the given combinations) that make use of these MRM transitions. The figure below shows each MRM transition required to identify PC (18:1/16:0). The phospholipid can be inferred by combining the analytical results obtained from these MRM transitions.
Step 1 Simultaneous Analysis Using the Phospholipid Classification Method
The phospholipid classification method is capable of comprehensive analysis of the main phospholipids in biological samples. It determines the phospholipid class based on the main characteristic head groups of phospholipids. Its analysis targets are phospholipids that include C14 to C22 fatty acids.
Step 2 Peak Identification
The phospholipid peak detected by the phospholipid classification method is identified. The structural information on the detected phospholipid at this point is the phospholipid class (PC, PE, PG, PI, PS, SM), and the total carbon number and number of double bonds in its constituent fatty acids. In the next step, the fatty acid composition determination method is created.
Step 3 MRM Event-Link Editor Editing the Fatty Acid Composition Determination Method
Software allows a fatty acid composition determination method to be edited from 867 MRM transitions for the phospholipid peak detected during first analysis.
Step 4 Simultaneous Analysis by Fatty Acid Composition Determination Method
Edited in MRM Event-Link Editor, the fatty acid composition determination method is used to perform a second analysis on the same sample. Phospholipid profiling can be performed based on the results of the first and second analysis.
Step 5 Data Analysis
Based on the analysis results, it was determined that the sample contains PC (16:0/20:1) and PC (18:0/18:1), as shown in the figures below.
Remarks and Precautions
LabSolutions LCMS Ver. 5.109 or later and LabSolutions Insight Ver. 3.8SP1 or later are required.
LabSolutions is a trademark of Shimadzu Corporation or its affiliated companies in Japan and/or other countries.
For Research Use Only. Not for use in diagnostic procedures.
News / Events
ASMS (American Society for Mass Spectrometry) 2024
Anaheim Convention Center
MSACL 2024 (Mass Spectrometry Applications to the Clinical Lab)
Monterey Conference Center
National American Chemical Society (Spring) 2024
Ernest N. Morial Convention Center
New Orleans, Louisiana
Booth # 2931
Innovative Technology Developed by Shimadzu and Washington University is Expected to Improve Healthy Life Expectancy
New Q-TOF LCMS-9050 Delivers Exceptional Mass Accuracy and Fast, Stable Polarity Switching
Achieve Effortless High-Speed High-Sensitivity Analysis With New Space-Saving LCMS-2050 Single Quadrupole Mass Spectrometer
Shimadzu Scientific Instruments introduces the LCMS-2050 Liquid Chromatograph Quadrupole Mass Spectrometer that features a significantly reduced size, while providing high speed and high sensitivity analysis. Designed for effortless operation, the LCMS-2050 mass spectrometer combines ease of use as an LC detector with excellent MS capabilities.