LC/MS/MS Method Package for Lipid Mediators

Ready-to-Use Analytical Conditions

LC/MS/MS Method Package for Lipid Mediators

Lipid mediators (bioactive lipids) have important physiological functions and have been associated with a wide variety of disorders ranging from diabetes to allergic diseases. This method package provides a simultaneous analysis method that encompasses 196 compounds of lipid mediators, including compounds derived from the arachidonic cascade and more.

UFMS Technology Covers Wide Range of Compounds

ESI negative ionization is commonly used for the measurement of fatty acids but in the case of important lipid mediators such as anandamide, ESI positive ionization is preferred because higher sensitivity is achieved. Traditional LC/MS methods are limited to single-polarity analysis, but Shimadzu's UFMS technology allows the LCMS-8050 to perform 5 msec high-speed polarity switching, enabling multiple classes of analytes to be analyzed in a single run without loss of data quality.

The compound panel includes 100 compounds derived from arachidonic acid, 26 compounds derived from EPA, 23 DHA-derived compounds, 11 ethanolamides and 36 components that are metabolites of other fatty acids as well as platelet activating factors (PAF).

Correction for Quantitative Value and Retention Time Using 16 Internal Standards

LC/MS/MS Method Package for Lipid Mediators

The 196 compounds were divided into 18 groups based on their chemical properties and each internal standard was appropriately selected for each group. The use of internal standards allows for accurate peak identification and quantitation

Index of compounds

LC/MS/MS Method Package for Lipid Mediators - index of compounds

Category codes


linoleic acid


adrenic acid


docosahexaenoic acid


α-linolenic acid


dihomo-γ-linolenic acid




eicosadienoic acid


eicosapentaenoic acid


internal standard


arachidonic acid


Remarks and Precautions

  1. For Research Use Only. Not for Use in Diagnostic Procedures.
  2. Shimadzu makes no warranty regarding the accuracy of information included in the database or the usefulness of information obtained from using the database.

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