LC/MS/MS Method Package for Bile Acids

For LabSolutions LCMS - LC/MS/MS Method Package for Bile Acids

Around 12 million people worldwide suffer from chronic or acute liver failure, according to an estimate by the World Health Organization. Tests for enzymes in the blood, such as alanine aminotransferase and aspartate aminotransferase, are widely used for early diagnosis of liver disease and evaluating overall liver function. These tests are often used in conjunction with an assessment of all bile acids. As a result, there is a demand to carry out simultaneous assays for an individual bile acid as well as total bile acids.

Ready to Use Analytical Conditions

LC/MS/MS Method Packages available from Shimadzu deliver conditions for efficient and simultaneous multi-component analysis. This method package contains MRM transitions and retention times for 28 targeted bile acids along with 10 internal standards. Isobaric compounds are baseline separated as well. A simple plasma preparation protocol is included as well.

MRM Chromatogram for 28 bile acids and 10 internal standards

MRM Chromatogram for 28 bile acids and 10 internal standards

Compound List

Compound Name Abbreviation Formula Monoisotopic Mass
Tauro-a-muricholic acid TαMCA C26H45NO7S 515.29
Tauro-b-muricholic acid TβMCA C26H45NO7S 515.29
Tauroursodeoxycholic acid TUDCA C26H45NO6S 499.30
Taurohyodeoxycholic acid THDCA C26H45NO6S 499.30
Taurocholic acid※ TCA C26H45NO7S 515.29
Taurocholic acid-2,2,3,4,4-d5 D5-TCA C26H40NO7S d5 520.32
Taurochenodeoxycholic acid※ TCDCA C26H45NO6S 499.30
Taurochenodeoxycholic acid- 2,2,4,4 -d4 D4-TCDCA C26H41NO6S d4 503.32
Taurodeoxycholic acid TDCA C26H45NO6S 499.30
Taurolithocholic acid※ TLCA C26H45NO5S 483.30
Taurolithocholic acid-2,2,3,4,4-d5 D5-TLCA C26H40NO5S d5 488.33
Glycocholic acid※ GCA C26H43NO6 465.31
Glycocholic acid-2,2,4,4 -d4 D4-GCA C26 H39NO6d4 469.33
Glycoursodeoxycholic acid GUDCA C26H43NO5 449.31
Glycohyodeoxycholic acid GHDCA C26H43NO5 449.31
w-Muricholic acid ωMCA C24H40O5 408.29
a-Muricholic acid αMCA C24H40O5 408.29
7-keto-Deoxycholic acid 7-keto-DCA C24H38O5 406.27
b-Muricholic acid βMCA C24H40O5 408.29
Glycochenodeoxycholic acid GCDCA C26H43NO5 449.31
Cholic acid※ CA C24H40O5 408.29
Cholic acid-2,2,4,4-d4 D4-CA C24 H36O5d4 412.31
Glycodeoxycholic acid※ GDCA C26H43NO5 449.31
Glycodeoxycholic acid-2,2,4,4 -d4 D4-GDCA C26H39NO5d4 453.34
Ursodeoxycholic acid UDCA C24H40O4 392.29
Hyodeoxycholic acid HDCA C24H40O4 392.29
7-keto-Lithocholic acid 7-keto-LCA C24H38O4 390.28
12-keto-Deoxycholic acid 12-keto-LCA C24H38O4 390.28
Chenodeoxycholic acid※ CDCA C24H40O4 392.29
Chenodeoxycholic acid-2,2,4,4-d4 D4-CDCA C24H36O4d4 396.32
allo-Chenodeoxycholic acid allo-CDCA C24H40O4 392.29
Deoxycholic acid※ DCA C24H40O4 392.29
Deoxycholic acid-2,2,4,4-d4 D4-DCA C24H36O4d4 396.32
Glycolithocholic acid※ GLCA C26H43NO4 433.32
Glycolithocholic acid-2,2,4,4-d4 D4-GLCA C26H39NO4d4 437.34
Lithocholic acid※ LCA C24H40O3 376.30
Lithocholic acid-2,2,4,4-d4 D4-LCA C24H36O3d4 380.32
allo-Lithocholic acid allo-LCA C24H40O3 376.30

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