High Performance Liquid Chromatography (HPLC & UHPLC)


Shimadzu HPLCi-Series HPLC Nexera LC-40

Get the unmatched performance and data quality you need from your HPLC systems

Shimadzu has been at the forefront of HPLC/UHPLC design and manufacturing for more than 40 years, developing instruments that enable chromatographers to select the system best suited to their application. Whether used for routine liquid chromatography analysis or high-end research, our versatile portfolio of instruments, from micro LC and analytical HPLC/UHPLC to SFC and preparative HPLC, delivers unmatched performance and data quality. In addition, with the incorporation of Analytical Intelligence, these systems simplify operation and enable more efficient workflows.

Analytical HPLC/UHPLC Systems

Nexera Series - Modular UHPLC/HPLC Systems

Nexera Series - Modular UHPLC/HPLC Systems

Whether your need is routine LC analysis, ultrafast separations, or high resolution, the modular Nexera (U)HPLC series offers the versatility to meet your requirements. Combining outstanding performance features with advancements in Analytical Intelligence, the Nexera (U)HPLC sets new standards for throughput, uptime, and efficiency.
i-Series Integrated HPLC and UHPLC Systems

i-Series Integrated HPLC and UHPLC Systems

Redefining integrated LC technology by adding innovative, intelligent and intuitive features to existing performance excellence, the new integrated i-Series HPLC/UHPLC delivers outstanding data quality, improved workflow efficiency, and maximum uptime.
Nexera Prep Preparative HPLC

Nexera Prep HPLC

Nexera Prep LC systems and components enhance all aspects of the separation and purification process, delivering excellent purity results, processing speed, and cost-effectiveness. Versatile hardware configurations, easy optimization of preparative parameters, and a user-friendly workflow allow users to select the system that best matches the target fraction volume and sample type.

IC – Ion Chromatography

Prominence Ion Chromatograph Anion Suppressor Ion Chromatograph

Ion chromatography systems (IC)

Shimadzu's HIC-ESP ion chromatograph features the same low carry-over, excellent injection precision and low pump pulsation characteristics of the standard HPLC systems, to ensure highly reliable results in quantitative ion analysis. The HIC-ESP system is ideal for ionic samples from environmental testing, pharmaceutical or chemical analysis, medical devices or food science.
ICDS-40A anion suppressor


The ICDS-40A anion suppressor is a highly efficient, electrodialytic membrane suppressor for ion chromatography. It offers stable reduction of electrical conductivity of the eluent at the time of detection, enabling highly sensitive anion analysis. It features a low internal volume resulting in minimized peak dispersion for improved peak shape and good separation of early eluting compounds.

SFC – Supercritical Fluid Chromatography

Analytical SFC Systems

Analytical SFC Systems

Combining on-line SFE and SFC in a single flow path, Nexera UC eliminates the need for complicated sample preparation and enables highly reliable and stable analysis of delicate samples. Using a mobile phase of supercritical carbon dioxide, the Nexera UC system offers high separation speed, excellent chromatographic resolution, and a high target analyte recovery rate while reducing the quantity of organic solvents used. Multiple configurations are available.
Preparative SFC Systems

Preparative SFC Systems

A next-generation solution to the demand for efficient and robust semi-prep SFC purification in the pharmaceutical, chemical and food industries, the Nexera UC Prep SFC system delivers outstanding data quality through the patented “LotusStream” gas-liquid separator technology. Its flexible system configuration in a compact design, combined with dedicated software, allows users to overhaul their workflow, reduce inefficiencies and meet a wide range of purification requirements.

GPC - Gel Permeation Chromatography

 Modular GPC System

Modular GPC System

The modular set-up of the Nexera GPC is the ideal tool for the separation of polymers by molecular weight using gel permeation chromatography (GPC). Based on the Nexera series HPLC systems, it is characterized by excellent retention time stability and flexible detection options, while the built-in Analytical Intelligence features ensure maximum reliability and ease-of-use.

Compact GPC system

Compact GPC system

Addition of a refractive index detector (RID) to a compact HPLC builds an i-series GPC, a conventional scale GPC system using multiple detectors with a minimized footprint. A recycle valve kit can be attached to the RID to return column eluate to the mobile phase bottle during intervals when no component peaks are eluted. This reduces mobile phase consumption, cost of analysis per sample and the environmental impact.

Analytical HPLC/UHPLC Components

HPLC Solution System Packages

Method Transfer System

Nexera-i MT Method Transfer System

Providing easy, seamless method migration in one instrument
Method Scouting System

HPLC/UHPLC Method Scouting System

Automatic column, eluent scouting in analytical methods development

HPLC Application Specific Systems

Application specific systems for the analysis of cannabis, hemp, bioethanol, and more

Application Specific Analyzers based on the i-Series Plus

Application specific systems for the analysis of cannabis, hemp, bioethanol, and more

HPLC Knowledge Base

Nexera UHPLC - learn more

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