Nexera Prep SFC Preparative Supercritical Fluid Chromatography System

 

Unique LotusStream separator technology achieves higher recovery rates

In fractionation purification using SFC, the target compounds are recovered in high concentrations in organic solvent, resulting in quick dry-down times when isolating collected samples. This is a great time saver. The Nexera Prep SFC with LotusStream also produces the highest recoveries the first time, every time.

 

High recovery rates

In preparative SFC, one factor that results in lower recovery rates is increased dispersion of the eluant when the CO2 returns suddenly from a supercritical to a gaseous state. The  patented LotusStream gas-liquid separator successfully reduces sample dispersion and carryover, while also achieving high recovery rates. High recoveries can be obtained regardless of flow rate or modifier concentration, even for volatile compounds such as the fragrance linalool.

 
LotusStream separator Decreases flow speeds without increasing the tubing diameter
Comparison for 1% linalool 
 
Equipment Recovery rate
Conventional separator 78.0%
LotusStream 96.7%
 

LotusStream separator (patented technology)

The LotusStream gas-liquid separator reduces flow speeds by splitting flow through multiple flow channels. The CO2 is discharged externally while the liquid travels along the column and drips directly below in a focused stream.

 
 
 

Prep Solution enables a seamless preparative workflow

The dedicated software, Prep Solutions, ensures that it is simple to scale up from an analytical to a preparative workflow, and makes it easy to configure parameter settings. It greatly increases the efficiency of preparative purification workflows.

 

Easy to understand even for first-time users

The parameter settings in Prep Solutions have been kept as simple and intuitive as possible, so that all users can operate the system without confusion. This also avoids the risk of wasting samples through human error.

 
1) Single analysis (peak check)

 

A single analysis prior to fractionation confirms the peak elution positions. Analysis can be started by simply inputting basic parameters in the three onscreen tabs.

2) Simulation

The chromatogram obtained from the single analysis can be displayed in the simulation window, so that fraction ranges can be selected by eye using the mouse. These settings can be applied to methods automatically.

 
 

Simulation window software screen

3) Fractionation

 

Samples are fractionated based on the user-selected parameters. The fraction range is displayed on the chromatogram, which can be checked in real time.

4) Adjust parameters during fractionation

Parameter settings for fractionation and injection can be adjusted during stacked injection (“on-the-fly” function).

 
 

Easily isolate the target peaks software screen

 

Compact, benchtop design

This space-saving benchtop model includes a carbon dioxide pump that does not require an external chiller (cooling system for the heat source). In addition, one unit can handle multiple flow volumes, lowering installation costs.

Benchtop system that can be installed anywhere

Usually a chiller is required to cool the solvent delivery pump when pumping CO2 at high flow rates. However, the Nexera Prep SFC features a compressor-type cooling unit, reducing the size of the system and allowing it to be installed anywhere. Its footprint is equivalent to an analysis-scale SFC system.

Conventional preparative SFC system versus Nexera UC Prep reduced footprint

 
 

Choose the optimal configuration for your application

Stacked Fraction System: supports injection volumes up to 20 mL for high flow rates

This system is optimized for high preparative flowrates that involve repeatedly injecting compounds which may contain several components. It can be optimized not only for chiral compounds but also achiral compounds. The FRS-40SF unit includes both injector and fraction collector functionality, so that the same unit can be used for repeated sample injections and gram-level preparative work. It supports injection volumes up to 20 mL* and ten recovery columns. Compatible with flow rates from 10 to 150 mL/min and 10 to 30 mm I.D. columns. *optional

Stacked Fraction Purification System supports injection volumes up to 20 mL for high flow rates

 

Multi-Fraction System: for multiple peak fractionation of impurities and natural substances

This system is suitable for preparative tasks involving samples with many peaks detected, such as impurities in pharmaceuticals. Volumes of up to 2 mL* can be injected using an autosampler that holds up to 162 samples (using 1.5 mL vials). Three rack types can be selected for the FRC-40 fraction collector, which can recover up to 540 fractions (using 10 mL vials). With support for flow rates from 10 to 150 mL/min, columns with an internal diameter from 10 to 30 mm can be used. *optional

Multi-Fraction System: for multiple peak fractionation of impurities and natural substances

Analytical Fraction System: analysis & low flow rate compatibility in one system

This system is intended for analysis-scale flowrates for applications that only require fraction volumes up to a few mg (used to check synthesis for example). By connecting an FRC-40 SF fraction collector to the Nexera UC system, the system can seamlessly perform everything from analysis to preparative work. With a flow rate range of up to 5 mL/min, analysis-scale columns can be used.

 
 
 
 Product lineup
 
  Stacked Fraction System Multi-Fraction System Analytical Fraction System
Flow rate range   10.0~150.0 mL/min 10.0~150.0 mL/min 0.0001~5.0 mL/min
Supported columns I.D:10mm~30mm
Length:up to 250mm
I.D.:10mm~30mm
Length:up to 250mm
I.D.:2.0~4.6mm
Length:up to 250mm
Injection unit
No. of samples processed: 1
Max. injection volume: 2 mL
(optional: 20 mL)
No. of samples processed:
252 (with 1 mL sample vial plate)
162 (1.5 mL sample vial plate)
84 (4 mL sample vial plate)
36 (10 mL sample vial plate)
72 (microtube plate)
288 (96-well microplate)
1,152 (384-well microplate)
Max. injection volume: 500 μL
(optional: 2 mL)
No. of samples processed:
175 (with 1-mL sample vial plate)
105 (1.5-mL sample vial plate)
50 (4-mL sample vial plate)
192 (96-well microplate)
768 (384-well microplate)
192 (96-well deep-well plate)
768 (384-well deep-well plate)
Max. injection volume: 5 μL
(optional: 20 μL)
Recovery
Supported containers: Bottles (screw-top: GL45)
Quantity: Five 100 mL to 2 L
Recovery method: Valve switching
(10 collection + 1 waste or 5 collection + 5 waste)
Supported containers: Test tubes
Quantity: 540 10-mL, 20 250-mL, or 12 500-mL test tubes
Test tube I.D.: From 10 mm
Recovery method: Arm action
Supported containers: Test tubes
Quantity: 540 10-mL, 20 250-mL,
or 12 500-mL test tubes
Test tube I.D.: From 10 mm
Recovery method: Arm action
Volume recovered per analysis
(approx.)
Up to 1 g (for 30 mm I.D. column)
Up to 1 g (for 30 mm I.D. column)
Up to 20 mg
(for 4.6 mm I.D. column)
Sample temperature
control range
No temperature control
4~45°C 4~40°C
Support functions Dedicated preparative software,
stacked injection,
parameter changing during analysis
Dedicated preparative software,
stacked injection,
parameter changing during analysis
Dedicated preparative software,
parameter changing during analysis
 

For Research Use Only. Not for use in diagnostic procedures.

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