Nexera Prep SFC Preparative Supercritical Fluid Chromatography System

Nexera Prep

The Nexera Prep SFC is a new preparative supercritical fluid chromatography system that builds on the successful analytical-scale Nexera UC with innovative, state-of-the-art preparative purification SFC technologies.

 
  • Designed in collaboration with the Enabling Technologies Consortium (ETC)
  • Addresses historical issues in SFC prep purification
  • Creates new benchmarks in fraction recovery and low carryover
  • Minimizes bench footprint in a truly compact design

Two Workflow Goals – Two Configurations

New Prep Purification SFC w/FRS-40 – Stacked Fraction System

  • For the repetitive purification of large volumes into a single bottle set (up to 10)
  • Perfect for both chiral and achiral separations
  • LotusStream™ Gas-Liquid Separator on each collection bottle
  • Sample injection volumes up to 20 mL; flow rates from 10 to 150 mL/min.
  • Supports stacked injections

Stacked Fraction Purification System supports injection volumes up to 20 mL for high flow rates

 

New Prep Purification SFC w/FRC-40 SF – Multi-Fraction System

  • For multiple peak fractionation of impurities and natural substances
  • Perfect for both chiral and achiral separations
  • LotusStream™ Gas-Liquid Separator integral to the collection head
  • Sample injection volumes up to 2 mL; flow rates from 10 to 150 mL/min.

Multi-Fraction System: for multiple peak fractionation of impurities and natural substances

In fractionation purification using SFC, the target compounds are recovered in high concentrations in organic solvent, resulting in quick dry-down times when isolating collected samples. This is a great time saver. The Nexera Prep SFC with LotusStream also produces the highest recoveries the first time, every time.

 

High recovery rates

In preparative SFC, one factor that results in lower recovery rates is increased dispersion of the eluant when the CO2 returns suddenly from a supercritical to a gaseous state. The  patented LotusStream gas-liquid separator successfully reduces sample dispersion and carryover, while also achieving high recovery rates. High recoveries can be obtained regardless of flow rate or modifier concentration, even for volatile compounds such as the fragrance linalool.

 
LotusStream separator Decreases flow speeds without increasing the tubing diameter
Comparison for 1% linalool 
 
Equipment Recovery rate
Conventional separator 78.0%
LotusStream 96.7%
 

LotusStream separator (patented technology)

The LotusStream gas-liquid separator reduces flow speeds by splitting flow through multiple flow channels. The CO2 is discharged externally while the liquid travels along the column and drips directly below in a focused stream.

Direct fractionation into vials

Direct fractionation into 96-well plate

 
 
 

Prep Solution enables a seamless preparative workflow

The dedicated software, Prep Solutions, ensures that it is simple to scale up from an analytical to a preparative workflow, and makes it easy to configure parameter settings. It greatly increases the efficiency of preparative purification workflows.

 

Easy to understand even for first-time users

The parameter settings in Prep Solutions have been kept as simple and intuitive as possible, so that all users can operate the system without confusion. This also avoids the risk of wasting samples through human error.

 
1) Single analysis (peak check)

 

A single analysis prior to fractionation confirms the peak elution positions. Analysis can be started by simply inputting basic parameters in the three onscreen tabs.

2) Simulation

The chromatogram obtained from the single analysis can be displayed in the simulation window, so that fraction ranges can be selected by eye using the mouse. These settings can be applied to methods automatically.

 
 

Simulation window software screen

3) Fractionation

 

Samples are fractionated based on the user-selected parameters. The fraction range is displayed on the chromatogram, which can be checked in real time.

4) Adjust parameters during fractionation

Parameter settings for fractionation and injection can be adjusted during stacked injection (“on-the-fly” function).

 
 

Easily isolate the target peaks software screen

 

Compact, benchtop design

This space-saving benchtop model includes a carbon dioxide pump that does not require an external chiller (cooling system for the heat source). In addition, one unit can handle multiple flow volumes, lowering installation costs.

Benchtop system that can be installed anywhere

Usually a chiller is required to cool the solvent delivery pump when pumping CO2 at high flow rates. However, the Nexera Prep SFC features a compressor-type cooling unit, reducing the size of the system and allowing it to be installed anywhere. Its footprint is equivalent to an analysis-scale SFC system.

Conventional preparative SFC system versus Nexera UC Prep reduced footprint

 

Analytical Fraction System: analysis & low flow rate compatibility in one system

This system is intended for analysis-scale flowrates for applications that only require fraction volumes up to a few mg (used to check synthesis for example). By connecting an FRC-40 SF fraction collector to the Nexera UC system, the system can seamlessly perform everything from analysis to preparative work. With a flow rate range of up to 5 mL/min, analysis-scale columns can be used.

 
 
 
 Product lineup
 
  Stacked Fraction System Multi-Fraction System Analytical Fraction System
Flow rate range   10.0~150.0 mL/min 10.0~150.0 mL/min 0.0001~5.0 mL/min
Supported columns I.D:10mm~30mm
Length:up to 250mm
I.D.:10mm~30mm
Length:up to 250mm
I.D.:2.0~4.6mm
Length:up to 250mm
Injection unit
No. of samples processed: 1
Max. injection volume: 2 mL
(optional: 20 mL)
No. of samples processed:
252 (with 1 mL sample vial plate)
162 (1.5 mL sample vial plate)
84 (4 mL sample vial plate)
36 (10 mL sample vial plate)
72 (microtube plate)
288 (96-well microplate)
1,152 (384-well microplate)
Max. injection volume: 500 μL
(optional: 2 mL)
No. of samples processed:
175 (with 1-mL sample vial plate)
105 (1.5-mL sample vial plate)
50 (4-mL sample vial plate)
192 (96-well microplate)
768 (384-well microplate)
192 (96-well deep-well plate)
768 (384-well deep-well plate)
Max. injection volume: 5 μL
(optional: 20 μL)
Recovery
Supported containers: Bottles (screw-top: GL45)
Quantity: Five 100 mL to 2 L
Recovery method: Valve switching
(10 collection + 1 waste or 5 collection + 5 waste)
Supported containers: Test tubes
Quantity: 540 10-mL, 20 250-mL, or 12 500-mL test tubes
Test tube I.D.: From 10 mm
Recovery method: Arm action
Supported containers: Test tubes
Quantity: 540 10-mL, 20 250-mL,
or 12 500-mL test tubes
Test tube I.D.: From 10 mm
Recovery method: Arm action
Volume recovered per analysis
(approx.)
Up to 1 g (for 30 mm I.D. column)
Up to 1 g (for 30 mm I.D. column)
Up to 20 mg
(for 4.6 mm I.D. column)
Sample temperature
control range
No temperature control
4~45°C 4~40°C
Support functions Dedicated preparative software,
stacked injection,
parameter changing during analysis
Dedicated preparative software,
stacked injection,
parameter changing during analysis
Dedicated preparative software,
parameter changing during analysis
 

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