HiPLEX-IHC Imaging on an Entry-Level Benchtop MALDI-TOF Mass Spectrometer

Overview

Title: HiPLEX-IHC Imaging on an Entry-Level Benchtop MALDI-TOF Mass Spectrometer

Duration: 41 minutes

Available On Demand

Summary

In recent years, Matrix assisted laser desorption ionization immunohistochemistry (MALDI-IHC) has become a popular technique in imaging. MALDI-IHC uses a photocleavable peptide mass tag conjugated with an antibody to target antigens within a sample. Subsequent cleavage of the mass tag allows for this to be detected by MALDI-TOF. Using mass spectrometry imaging (MSI), spatial information of the cleaved peptide can be determined and, from this, the locations of the targets in the tissue, can be inferred. In this webinar, we will cover some of the basics on what MALDI is and how it can be used as a tool for imaging before exploring MALDI-IHC in more detail including some examples of its implementation on our entry level 8000 series linear MALDI-TOF benchtop mass spectrometer. Our aim is to show how easy it can be to incorporate MALDI imaging into your laboratory, especially if you currently use immunohistochemistry workflows. We are ready to support you wherever you may be on your MALDI imaging journey.

Video