HPLC/UHPLC Evaporative Light Scattering Detector

ELSD-LT III

HPLC/UHPLC Evaporative Light Scattering Detector ELSD-LT III

Experience robust, sensitive detection of both chromophoric and non-chromophoric molecules. The low temperature ELSD-LT III is a universal detector that is a wonderful complement to any HPLC laboratory.  ELSD measures the light scattered by particle aggregates of analytes that persist after an evaporation of the mobile phase matrix.

 

Request a Quote  Get Support 

 
 

Why Evaporative Light Scattering  Detection?

  • Universal detection.
  • Total gradient compatibility.
  • High sensitivity (ng – μg range).
  • No equilibration time.
  • Compatibility with LC-MS mobile phases.
  • Essential detection compliment to UV and MS.
  • Dynamic Range Extension for the quantitation of high and low concentrations in the same run.
  • Controlled via Shimadzu LabSolutions software.
  • Stacks neatly onto the CTO-40C column oven.

Principles of Operation

Evaporative Light Scattering Detector Principles of Operation Diagram

Common ELSD-LT Applications

 

Excipients

Synthetic Mix of PEG MW 200, 300, 400, 600, 1000 and 2000​

 
Synthetic Mix of PEG MW 200, 300, 400, 600, 1000 and 2000​ Chromatogram

Column:C18, 1.7 μm, 100x 3.0 mm ID
Mobile Phase: A: Water B: Acetonitrile
Gradient: B. conc. 15% to 31% (0-4 min), 31% (4-4.5 min), 31% to 40% (4.5 – 9 min)
Flow Rate: 1.0 m L/ min 
Detector: ELSD-100 LT (Gain Wide; 45C filter 1 sec, 350 kPa )

 
 

Carbohydrates

Mono-, Di- and Oligosaccharide Standards

Mono-, Di- and Oligosaccharide Standards Chromatogram
  1. Glycerol
  2. Arabinose
  3. Fructose
  4. Glucose
  5. Sucrose
  6. Maltose
  7. Maltotriose
  8. Maltotetraose
  9. Maltopentaose
  10. Maltohexaose
  11. Maltoheptaose

Column: Premier Carbohydrate, 5 mm, 250x 4.6mm
Mobile Phase: A: Acetonitrile B: 0.0004N NH4OH
Gradient: (Time, %B)(0,15)(60,65)
Flow Rate: 1.0m L/ min
Col. Temp: 30ºC
Detector: Shimadzu ELSD -LT (Gain 5; T 40ºC; P 250kPa)

For Research Use Only. Not for use in diagnostic procedures.

Top of This Page