HPLC/UHPLC Evaporative Light Scattering Detector
ELSD-LT III
Experience robust, sensitive detection of both chromophoric and non-chromophoric molecules. The low temperature ELSD-LT III is a universal detector that is a wonderful complement to any HPLC laboratory. ELSD measures the light scattered by particle aggregates of analytes that persist after an evaporation of the mobile phase matrix.
Why Evaporative Light Scattering Detection?
- Universal detection.
- Total gradient compatibility.
- High sensitivity (ng – μg range).
- No equilibration time.
- Compatibility with LC-MS mobile phases.
- Essential detection compliment to UV and MS.
- Dynamic Range Extension for the quantitation of high and low concentrations in the same run.
- Controlled via Shimadzu LabSolutions software.
- Stacks neatly onto the CTO-40C column oven.
Principles of Operation
Common ELSD-LT Applications
Excipients
Synthetic Mix of PEG MW 200, 300, 400, 600, 1000 and 2000
Column:C18, 1.7 μm, 100x 3.0 mm ID
Mobile Phase: A: Water B: Acetonitrile
Gradient: B. conc. 15% to 31% (0-4 min), 31% (4-4.5 min), 31% to 40% (4.5 – 9 min)
Flow Rate: 1.0 m L/ min
Detector: ELSD-100 LT (Gain Wide; 45C filter 1 sec, 350 kPa )
Carbohydrates
Mono-, Di- and Oligosaccharide Standards
- Glycerol
- Arabinose
- Fructose
- Glucose
- Sucrose
- Maltose
- Maltotriose
- Maltotetraose
- Maltopentaose
- Maltohexaose
- Maltoheptaose
Column: Premier Carbohydrate, 5 mm, 250x 4.6mm
Mobile Phase: A: Acetonitrile B: 0.0004N NH4OH
Gradient: (Time, %B)(0,15)(60,65)
Flow Rate: 1.0m L/ min
Col. Temp: 30ºC
Detector: Shimadzu ELSD -LT (Gain 5; T 40ºC; P 250kPa)